当归对宫内缺氧幼年大鼠海马CA3区神经元与学习能力的影响及机制作者：贺艳赵峰马晶余鸿【摘要】目的探讨宫内缺氧对幼年大鼠海马CA3区神经元与学习能力的影响，以及NMDAR1mRNA在其新生期的表达与当归的调控作用。方法健康SD孕鼠30只，随机分为对照组、缺氧组和当归组各10只，于孕14d开始将缺氧组与当归组孕鼠用三气培养箱制作胎鼠宫内缺氧模型，当归组用250g/L当归静脉注射干预。3组孕鼠分娩当日每窝随机选取新生鼠2只，取脑、常规石蜡切片;余新生鼠随机选取2只喂养至30d进行Morris水迷宫测试，测试结束当日，多聚甲醛灌注固定取脑、常规石蜡切片。幼鼠脑组织作NSEmRNA原位杂交、新生鼠脑组织作NMDAR1mRNA原位杂交。结果Morris水迷宫实验和NSEmRNA原位杂显示：与对照组(n=20)相比，缺氧组幼鼠(n=20)空间探查测试和对位探查测试时间较缩短、海马CA3区阳性细胞数减少(P《0.05)，当归组(n=20)探查测试时间较缺氧组延长(P《0.05)、海马CA3区阳性细胞数增多(P《0.05);NMDAR1mRNA原位杂交显示：与对照组相比，缺氧组海马CA3区阳性细胞积分光密度值增大，当归组较缺氧组减小(P《0.05)。结论宫内缺氧可致新生大鼠海马CA3区NMDAR1mRNA表达增高，从而使幼年大鼠该区神经元减少，学习记忆能力---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---降低，而当归注射液可改善宫内缺氧状态，增加幼年大鼠海马CA3区神经元数量，提高其学习记忆能力。【关键词】海马;学习能力;N-甲基-D-天门冬氨酸受体1;缺氧;当归Abstract：ObjectiveToexploretheeffectofintrauterinehypoxiaonhippocampalCA3areaneuronsandlearningcapacityofjuvenilerats,andtoexploretheexpressionofN-methy-D-aspartatereceptor-1(NMDAR1)inhippocampalCA3areaofneonatalratsfollowingintrauterinehypoxiaandtheregulatorymechanismofAngelicasinensis.MethodsThirtyhealthypregnantSDratsweredividedrandomlyintocontrolgroup,hypoxiagroupandAngelicagroup.HypoxiagroupandAngelicagrouppregnantratsweremadetheintrauterinehypoxiamodeloffetalratswiththethree-gasincubatorfromthebeginningofpregnant14thday,andtheratsofAngelicagroupwereintervenedwithAngelicasinensisinjection.Afterbirth,twoneonatalratswereselectedrandomlyfromeachlittertotakethebraintissue,makeparaffinsectionsconventionly.TwoneonatalratswereselectedagainrandomlyfromtheothersineachlittertoperformofMorriswatermaze---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---afterthe30thdayofbirth,andperfusedparaformaldehydeattheendofthelastday,tookthebraintissueofjuvenilerats,andparaffinsectionsconventionly.TheexpressionofNSEmRNAandNMDAR1mRNAweredetectedbyusinginsitehybridization.ResultsInhypoxiagroupjuvenilerats(n=20),thesearchingtimeofprobetrialandreveralprobetrialinthetargetquadrant,andthequantityofpositiveNSEmRNAcellsinhyppocampalCA3areawerelessthanthatofcontrolgroup(n=20)(P《0.05);andthatofAngelicagroup(n=20)wasmorethanthatofhypoxiagroup(P《0.05).Theintegralopticaldensity(IOD)valueofpositiveNMDAR1mRNAcellsinhippocampalCA3areainhypoxiagroupneontalrats(n=20)wassignificantlyhigherthanthatofcontrolgroup(n=20)andalsohigherthanthatofAngelicagroup(n=20)(P《0.05).ConclusionIntrauterinehypoxiacanincreasetheexpressionofNMDAR1mRNAinhippocampaldentategyrusofneontalrats,thusreducedthequantityofhippocampaldentategyrusneuronsandlearning-memorycapacityofjuvenilerats,whileAngelicasinensisinjectioncanimprovetheintrauterinehypoxiccondition,thusincreasethequantityofhippocampaldentategyrusneuronsandlearning-memorycapacityofjuvenilerats.---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---Keywords：Hippocampus;Learningcapacity;NMDAR1;Hypoxia;Angelica宫内缺氧是胚胎发育中...