PCR技术检测结核杆菌的进展第9卷天津医科大学Vo1.9第4期JTMU2003No.4giotensinsystemandfibrinolyticfunction[J】.Circulation,1993,87(6):1969[14】MarreM,BernadetP,GalloisY.eta1.RelationshiDsbetweenangiotensinIconvertingenzymegenepolymorphism.plasmalevels,anddiabeticretinalandrenalcomplications[J】.Dia—betes.1994.43(3):384【15】BarnasU,SchmidtA.IllievichA.eta1.Evaluationofriskfac-torsforthedevelopmentofnephropathyinpatientswithID-DM:insertion/deletionangiotensinconvertingenzymegenepolymorphism,hypertensionandmetaboliecontrol[JJDia—betologia,1997,40(3):327[16】OhnoJ,KawazuS,TomonoS.Associationanalysesofthepolymorphismsofangiotensinconvertingenzymeandan—giotensingeneswithdiabeticnephropathyinJapanesenon—insulindependentdiabetic[J】.Metabolism,1996,45(2):218[17】MarreM,BouhanickB.BerrutG.eta1.Renalchangesonhy-perglycemiaandangiotensin——convertingenzymeintype1di—abetes[J】.Hypertension.1999,33(3):775[18】邵咏红,黄英伟,陈劲松,等.血管紧张素转换酶基因多态型与糖尿病及其肾脏合并症发病的关系[j】.中华肾脏杂志,1999,15(1):36[19】LevinsonO.OrenS,YaginC.Angiotensinconvertingenzyme(ACE)genepolymorphisminfldiabeticcohortanddiabeticnephropathy[J】.Harefuah,1999,136(10):768[20】FujisawaT,IkegamiH,KawaguchiY,eta1.Meta～analysisofassociationofinsertion/deletionpolymorphismofan-giotensinI—convertingenzymegenewithdiabeticnephropa—thyandretinopathy[JJ.Diabetologia,1998,41(1):47[21】ParvingHH,JacobsenP.TarnowL.eta1.Effectofdeletionpolymorphismofangiotensinconvertingenzymegeneonpro-gressionofdiabeticnephropathyduringinhibitionofan—giotensinconvertingenzyme:observationalfollowupstudy[J】.B~U,1996,313(7057):591【22】FaraS,AzzopardiJ.EllardS.eta1.ACEgenepolymorphismasaprognosticindicatorinpatientswithtype2diabetesandas-tablishedrenaldisease[J】.DiabetesCare2001,24(12):2115[23JGohdaT,MakitaY,ShikeT.etaI.AssociationoftheDDgenotpyeanddevelopmentofJapanesetype2diabeticnephropathy[J】.ClinNephrol2001,56(6):475[24】ArazM,YilmazN,GungorK,eta1.Anogiotensin—convertingenzymegenepolymorphismandmicrovascularcomplicationsinTurkishtype2diabeticpatients[J】.DiabetesResClinPract,2001.54(2):95[25】YoshidaH,MitaraiT,KawamaraT.eta1.RoleofthedeletionofpolymorphismoftheangiotensinconvertingenzymegeneintheprogressionandtherapeuticresponsivenessofIgAnephropathy[J】.jClinInvest,1995,96(5):2162f26】KitamuraH,MoriyamaT,IzumiM,eta1.AngiotensinI—convertingenzymeinsertion/deletionpolymorphism:potentialsignificanceinnephrology[J】.KidneyIntSuppl,1996,55:S101【27】JacobsenP,RossingK,RossingP,eta1.Angiotensinconvert-ingenzymegenepolymorphismandACEinhibitionindiabeticnephropathy[J】.KidneyInt,1998,53(4):1002(2003—01—07收稿)PCR技术检测结核杆菌的进展杨晶综述,张乃鑫审校(天津医科大学病理学教研室,天津300070)[关键词】聚合酶链反应;结核杆菌;进展[中图分类号】R52[文献标识码】A[文章编号】1006—8147(2003)04—0566～04用聚合酶链反应(polymeraschainreaction,PCR)技术检测结核杆菌的报道国外始见于1989年nI;10多年来,应用分子生物学技术检测结核杆菌的研究工作取得了许多进展,部分已应用于临床,近年来,在一般PCR扩增技术的基础上,开发了若干更加敏感的新方法.1Roche结核分支杆菌PCR实验和基因探针扩增作者简介:杨晶(1974一),女,博士研究生在读,研究方向:淋巴结病理.分支杆菌直接实验1.1Roche结核分支杆菌PCR实验(RocheAm—plicorPCR)由瑞士Roche公司开发,以生物素标记位于结核杆菌(Mycobacteriumtuberculosis)l6SrRNA基因中高度保守区域的种特异性引物,用来扩增MBTCDNA中的一段584bp大小...