1DigestiveSystemDepartment,People'sHospitalofXinyu,Xinyu338025,激angxiProvince,China;2InstituteofDigestiveDisease,theFirstAffiliatedHospitalofNanchangUniversity,Nanchang330006,激angxiProvince,ChinaLiuWei★,Master,Physician,DigestiveSystemDepartment,People'sHospitalofXinyu,Xinyu338025,激angxiProvince,China;InstituteofDigestiveDisease,theFirstAffiliatedHospitalofNanchangUniversity,Nanchang330006,激angxiProvince,Chinabaibianfuliuwei@163Correspondenceto:XieYong,Professor,Chiefphysician,InstituteofDigestiveDisease,theFirstAffiliatedHospitalofNanchangUniversity,Nanchang330006,激angxiProvince,Chinaxieyong_med@163Supportedby:theNationalNaturalScienceFoundationofChina,No.30460052*Received:2010-10-05Accepted:2011-03-12新余市人民医院消化内科，江西省新余市中国组织工程研究与临床康复第15卷第00期2011–00–00出版JournalofClinicalRehabilitativeTissueEngineeringResearchMay00,2011Vol.15,No.00幽门螺杆菌全菌蛋白抗原壳聚糖微球体外释放特性*★刘微1,2，谢勇2，刘志军1PreparationandinvitroreleasecharacteristicsofchitosanmicrospheresloadingHelicobacterpyloriwholecellproteinantigenLiuWei1,2,XieYong2,LiuZhi-jun1AbstractBACKGROUND:ChitosanmicrospheresloadingHelicobacterpyloriwholecellproteinantigenpreparedbydifferentmethodshavedifferentthepackageratesandcontrolled-releaseeffects.OBJECTIVE:TopreparechitosanmicrospheresloadingHelicobacterpyloriwholecellproteinantigenandobservethereleasecharacteristicsinvitro.METHODS:Bertholdprecipitationmethodwasusedtopreparechitosanmicrospheresandthepreparationwasoptimized;scanningelectronmicroscopeandpsizeanalyzerwereusedtoobservetheshapeandsizemeasurementofchitosanmicrospheres;theBCAProteinAssayKitwasusedtomeasuretheloadingefficiency,loadingcapacityandreleasingrateofmicrospheres.RESULTSANDCONCLUSION:Wechosethebestpreparationprogramofchitosanmicrospheresfrom32speciespreparationprograms:thebestpreparationprogramwasthematerialofseashellchitosan,1%concentrationofaceticacid,sodiumsulfateforprecipitatingagentand5.0pHvalue,non-useofultrasonictreatment.SEMresultsshowedthatthemicrospherespresentedwithsmoothroundanddense,diametersrangedfrom1.0μmto5.0μm;Antigenloadingefficiencywas79.92%,loadingcapacitywas16.47%;Releaseexperimentinvitroshowedthatthetotalantigenreleasingratewas20.39%,showingaslow-releasestatus.LiuW,XieY,LiuZJ.PreparationandinvitroreleasecharacteristicsofchitosanmicrospheresloadingHelicobacterpyloriwholecellproteinantigen.ZhongguoZuzhiGongchengYanjiuyuLinchuangKangfu.2011;15(00):0000-0000.[wwcrteren.zglckf]摘要背景：不同方法制备出的幽门螺杆菌全菌蛋白抗原壳聚糖微球，其包裹率和控释效果也不同。目的：探讨幽门螺杆菌全菌蛋白抗原壳聚糖微球的最佳制备方案，观察其体外释放特性。方法：使用Berthold沉淀法制备壳聚糖微球，筛选最佳制备方案；使用扫描电镜及粒径分析仪观察壳聚糖微球的形态及粒径分布；冻干后的壳聚糖微球包裹幽门螺杆菌全菌蛋白抗原，使用BCA蛋白定量试剂盒测量分析微球的抗原包裹率、包裹量及释放率。结果与结论：从32种壳聚糖微球制备方案中筛选出了以海得贝壳聚糖为原料、冰乙酸的浓度为1%、硫酸钠为沉淀剂、pH值为5.0、不进行超声处理方案为最佳制备方案，扫描电镜示微球光滑圆整、致密，粒径分布在1.0~5.0μm；抗原包裹率为79.92%，包裹量为16.47%；体外释放实验表明，总抗原释放率为20.39%，呈缓慢释放状态。关键词：壳聚糖；幽门螺杆菌；微球；包裹；释放doi:10.3969/j.issn.1673-8225.2011.29.003刘微，谢勇，刘志军.幽门螺杆菌全菌蛋白抗原壳聚糖微球体外释放特性[J].中国组织工程研究与临床康复，2011，15(00):0...