骨保护素对破骨细胞的影响程度分析[摘要]目的通过分析比较不同浓度的骨保护素（osteoprotegerin，OPG）对破骨细胞（osteoclasts，OC）活性的影响程度来研究2者在生理活性及生理功能之间的相关性。方法本次实验应用6周龄的雌性小鼠模型，添加不同浓度的骨保护素后通过抗酒石酸酸性磷酸酶（tartrateresistantacidphosphatase，TRAP）染色、细胞骨架F-actin染色及骨吸收陷窝的检测，观察OPG与OC之间的相关性。结果OC培养1h内即贴壁生长，并为展开且无大的多核OC存在。培养至第5天，较小的单核细胞逐渐开始相互融合，形成多核细胞且特征明显。OPG处理3d后，对照组细胞形态整体性良好，利用观察比较。随着OPG浓度的增大，实验组的多核细胞数逐渐减少，多核细胞数随着骨保护素（OPG）浓度的逐渐增加而减少，2者间呈现负相关的关系（r=0.516，P＜0.05）。且当骨保护素（OPG）浓度为50μg/L时，视野内仅存在少量多核细胞，模糊可见。培养结束后，OPG组多核细胞数随着OPG浓度的增大而逐渐减少，且OPG浓度为50ng/mL时仅有少量多核细胞可见；OPG的浓度为20、50μg/L时，OPG组的OC数较对照组显著减少（P<0.05），而当OPG的浓度为100μg/L时，OPG组的OC数较对照组呈极显著减少（P<0.01）；OPG实验组的骨吸收陷窝的面积、密度和减弱程度均与OPG的浓度呈正相关（r=0.459，P<0.05），且面积比较具有显著性差异（P<0.05）。结论不同浓度的OPG对破骨细胞的影响程度不同，且OPG的浓度越大其抑制性越强。[关键词]骨保护素；破骨细胞；吸收陷窝[中图分类号]R3[文献标识码]AAnalysisoftheinfluencedegreeofosteoprotegerinonosteoclastsLIUJian1，SONGHui2(1.Departmentoforthopedics,ShandongHospitalofShenglioilfieldinDongyingCity,Dongying257000,China.2.Departmentoforalandmaxillofacialsurgery,ShandongUniversityJinan250000，China.)[Abstract]:ObjectiveEffectsofdifferentconcentrationsofOPG(osteoprotegerin,OPG)onosteoclasts(osteoclasts,OC)thedegreeofinfluenceactivity,provideatheoreticalbasisforfurtherresearchOPGandphysiologicalfunctionsofOC.Methods6-week-oldfemalemicewereculturedinvitroonthebasisofaddingdifferentlevelsofOPG,byTRAPstaining,F-actincytoskeletonstainingandresorptioncorrelationdetectionnestwasobservedbetweenOPGandOC.ResultsOCculturethatisadherentgrowthwithin1h,andforthelaunchofalargemulti-coreandnoCOpresent.Culturedfor5days,thesmallermonocytesgraduallybegantofusionwitheachothertoformmultinucleatedcellsanddistinctivecharacteristics.OPGtreatmentafter3d,cellmorphologycontrolgroupoverallgooduseofobservationandcomparison.WithOPGconcentrationincreases,thenumberofcellsintheexperimentalgroupmulticoregraduallyreducethenumberofcellswithincreasingmulticoreosteoprotegerin(OPG)concentrationdecreased,showinganegativecorrelationbetweenthetworelationships（r=0.516，P＜0.05）.Andwhenosteoprotegerin(OPG)concentrationof50ng·mL-1,thereisonlyasmallamountofmultinucleatedcellswithinthefieldofvision,blurredvisible.Afterobservingtheculture,OPGGrouppolynuclearcellswithOPGconcentrationincreasesgraduallyreduced,andOPGconcentrationof50ng·mL-1isonlyafewmultinucleatedcellsvisible;concentrationofOPGwas20,50μg·L-1when,OCnumberOPGgroupsignificantlydecreasedsignificantlyreduced[OCthanthecontrolgroup,istheresultofcomparingwhattimeandunderwhattimecome?](P<0.05),andwhentheconcentrationofOPGwas100μg·L-1时,OCnumberOPGgroupthaninthecontrolgroupshowedsignificantreduction[andwhocompare?](P<001.);ByX2testandSpearmanrankcorrelationanalysis,theexperimentalgroupOPGboneresorptionlacunaearea,densityanddecreasethedegreeofconcentrationofOPGwerepositivelycorrelated,andtheareawasasignificantdifference(X2=15.119,r=0.459,P<0.05).ConclusionEffectsofdifferentc...