201411057论著DOI：10.16016/j.l000-5404.201411057慢性牙周炎患者唾液中miR-146a的表达及临床意义赵家珍，闫文娟，吴补领(510515广州，南方医科大学南方医院口腔科)［摘要］目的通过检测慢性牙周炎患者唾液中miR-146a的表达，以探讨miR-146a在慢性才周炎发病中的作用及意义。方法收集2012年1月至2013年8月期间南方医院口腔科收治的48例慢性牙周炎患者(其中男性28例，女性20例，平均年龄42岁)。同时选择20名性别、年龄匹配的健康志愿者作为对照纟R。采用实时荧光定MPCR技术检测上述人员睡液上清中miR-146a的表达水平。对治疗组患者(32例)进行牙周基础治疗，分别记录治疗前和治疗6周后患者的探诊深度(probingdepth,PD)临床附着丧失(attachmentloss,AL)及菌斑指数(plaqueindex,PLI)，并检测治疗后唾液上清液标*1PmiR-146a的表达。结果慢性牙周炎患者唾液上清液'PmiR-146a的表达水平(2.52±0.87)显著高于健康对照纽.(0.81±0.58)(PV0.01)。治疗组患者经过治疗后的牙周临床指数、唾液中miR-146a表达水平均显著降低(PV0.05)。唾液中miR-146a水平与PD(尸0.574,PV0.05)、AL(r=0.629,PVO.01)及PLI(r=0.758,PV0.01)均呈正相关。结论慢性牙周炎患者唾液上清中miR-146a表达水平明显高于正常人，并在芳周基础治疗后显著下降，提示唾液miR-146可作为牙周病防治及疗效评估的潜在靶分子。［关键词］慢性牙周炎；唾液；微小RNA；miR-146aExpressionandclinicalsignificanceofsalivamiR-146ainpatientswithchronicperiodontitisZhaoJiazhen,YanWenjuan,WuBuling(DepartmentofStomatology,NanfangHospital,SouthernMedicalUniversity,Guagnzhou,GuangdongProvince,510515,China)[Abstract]ObjectiveTodeterminetheexpressionofmiR-146asalivainpatientswithchronicperiodontitisandinvestigateitsclinicalvalue.MethodsForty-eightchronicperiodontitispatientsadmittedinourdepartmentfromJanuary2012toAugust2013and20age-andscx-matchcdhealthyvolunteerswereenrolledashealthycontrolswereinvestigated(including28malesand20femalesatameanageof42years).Real-timePCRwasperformedtodetecttheexpressionofsalivamiR-146ainthesesubjects.Thirty-twopatientswithchronicperiodontitisweretreatedwithinitialtherapyfor6weeks.Theirprobingdepth(PD),attachmentloss(AL)andplaqueindex(PLI)wererecordedbeforeandin6weeksaftertreatment.ResultsTheexpressionofsalivamiR-146awerehigherinchronicperiodontitispatientsthanthehealthycontrols(2.52±0.87vs0.81±0.58,P<0.01).Afterinitialtherapy,theMiR-146aexpressionandallperiodontalindexeswerereducedafterinitialtherapy(P<0.05).PositivecorrelationwasfoundbetweensalivamiR-146aexpressionwithperiodontalPD(r=0.574,P<0.05),AL(r=0.629,P<0.01)andPLI(r=0・75&P<0.01).ConclusionMiR-146aexpressionisobviouslystrongerinsalivaofpatientswithchronicperiodontitisthaninhealthypeople,andsignificantlydecreasedafterinitialtherapy,indicatingthatmiR-146amaybeconsideredasapotentialtargetforpreventionandtreatmentofchronicperiodontitis.[Keywords]chronicperiodontitis;saliva;microRNA;mir-146aCorrespondingauthor：WuBuling,Tel:86-20-61642021,E-mail：bulingwu@smu.cdu.cn[通信作者]吴补领，电话：(020)60142021,E-mail：bulingwu@smu.edu.cn[优先出版]httD：//www・cnki・net/kcms/detail/51・1095.R.20150121・1030.005.html(2015・0「21)---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除------本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---牙周炎是发生于牙周支持组织的炎症破坏性疾病。牙周炎的发病机制涉及多种因素，H前认为，宿主对菌斑细菌及其产物的过度免疫丿M答在牙周纽•织的破坏及疾病的慢性迁延中起重要作用⑴。近年来，微小RNA(microRNA,miRNA)被发现在许多疾病中异常表达,其在慢性炎症发病过程中的作用成为该领域的研究热点⑵。miRNA是一类高...