BDNF、TrkB在岛叶慢性电点燃大鼠海马中的表达及与学习记忆的关系徐文中王峰张培松任双来齐江华孙涛*（宁夏医科大学，宁夏回族自治区颅脑疾病重点实验室，银川750004）摘要：目的研究岛叶慢性电点燃癫痫模型大鼠的学习记忆功能，检测脑源性神经营养因子(brain-derivedneurotrophicfactor，BDNF)、酪氨酸蛋白激酶受体B(TyrosinereceptorkinaseB，TrkB)在海马组织中表达，探讨BDNF、TRKB与岛叶点燃大鼠学习记忆的关系。方法成年SD大鼠随机分为模型组、假手术组、空白对照组，建立大鼠岛叶慢性电点燃模型。用避暗试验方法检测岛叶慢性电点燃模型大鼠学习记忆功能，采用免疫组化和westernblot方法分别检测BDNF、TrkB蛋白在海马组织中表达水平，用RT-PCR检测两因子基因表达水平。结果岛叶慢性电刺激点燃成功后第1天（即0周），学习记忆能力点燃组与空白对照组、假手术组差异无统计学意义(P＞0.05）。点燃2周点燃组避暗试验结果的LT为(48.87±3.42s)、EN为（2.75±0.99次）、点燃3周点燃组避暗试验结果的LT为(48.71±2.77s)、EN为（2.75±1.15次），与对照组（即点燃0周）相比学习记忆功能增强（P＜0.05），点燃4周后与对照组比学习记忆功能明显下降（P＜0.05）。BDNF、TrkB表达均在24h达到高峰（P＜0.05），空白对照组、假手术组无明显变化。组内比较，峰值及增幅均出现在2~3周内（P＜0.01)，4周后较前几周组明显下降（P＜0.05)。结论岛叶电点燃大鼠学习记忆功能与BDNF、TrkB在海马中表达总变化趋势一致。BDNF、TrkB与岛叶点燃大鼠学习记忆功能密切相关。关键词：岛叶；癫痫；BDNF；TRKB；海马；避暗试验；学习记忆：文献标识码：ATheexpressionofBDNFandTrkBinrathippocampusandtherelationshipwithlearning-rememberingabilityinchronicelectronicignitionofinsularlobe.XUWen-zhong,WANGFeng,ZHANGPei-song,RENShuang-lai,QI激ang-hua,,SUNTao.NingxiaMedicalUniversity,KeyLabofCraniocerbralDisease,NingXiaHuiAutonomousRegion,Yinchuan750004,ChinaCorrespondingauthor：SUNTao，Email：suntao@nxmu.edu.【Abstract】ObjectiveTostudytheexpressionofactiveSkeletonAdjustmentProtein(BDNF、TrkB)inrat’sHippocampusinthemodelofinsularchronicelectricity-ignitingepilepsyandinvestigatetherelationbetweenBDNF、TrkBandlearning-rememberingabilityintheepilepticrat.Methods:TodivideadultSDratsrandomlyintomodel,shamandcontrolgroup,*收稿日期：2010-09-17基金项目：国家自然科学基金资助（30750014）；宁夏自然科学基金资助(NZ09114)通信作者：孙涛，E-mail：suntao@nxmu.edu---本文于网络，仅供参考，勿照抄，如有侵权请联系删除---respectively.ThePassiveavoidancetestwasemployedtotestlearning-rememberingabilityofratsandImmunohistochemistryandwesternblotwasusedtodeterminetheexpressionofBDNF、TrkBinthehippocampusandRT-PCRwasexploitedtocheckthegeneexpressionofthetwofactors.Result:Thereisnobigdifferenceoflearning-rememberingabilityofratsamongtheignitinggrouporcontrolgroupandshamgroups(P>0.05)inthefirstweekafterinsularchronicignitionwhenpassiveavoidancetestwasexamined.Theincreasedlearning-rememberingabilityappearedinthe2ndweek(LT:48.87±3.42s;EN:2.75±0.99），3rdweek(LT:48.71±2.77s;EN:2.75±1.15)aftertheignition(P<0.05).Itwasdeclinedinthe4thweek.AsfortheexpressionofBDNFandTrkBthepeakvalueoccurredinthe24hours(P<0.05)withintheexperimentalgroups,itkeptincreasinginthe2ndand3rdweekandthendroppedinthe4thweek,buttherewasnostatisticaldifferenceamongthecontrolandshamgroups(P>0.05).Conclusion:ThegeneraltendencyoftheexpressionofBDNF、TrkBinrathippocampusafterthechronicinsularelectricityignitionwasconsistentwiththelearning-rememberingabilityofepilepsyrats.BDNFandTrkBarecloselyassociatedwiththelearning-rememberabilityforepilepsyrats.Keywords:Insula,epile...