铁皮石斛及其冻干粉对免疫抑制小鼠的免疫调节作用差异研究张静朱雅琴祝宇龙摘要[目的]探讨铁皮石斛及其冻干粉对提升免疫抑制小鼠的免疫调节作用差异。[方法]120只SPF级昆明雄性小鼠分为空白对照组、环磷酰胺模型组、铁皮石斛组和铁皮石斛冻干粉组。两组给药组连续灌胃30d后，24h内模型组、铁皮石斛及其冻干粉组一次性腹腔注射200mg/kg的环磷酰胺免疫抑制造模。试验结束后测定胸腺系数、脾脏系数、足趾肿胀度，以及血清中的细胞因子IFN-γ、IL-2和IL-6的含量。[结果]铁皮石斛及其冻干粉均可以升高免疫抑制小鼠的胸腺和脾脏系数;增加免疫抑制小鼠的足趾肿胀度;上调免疫抑制小鼠血清中TNF-α、IFN-γ、IL-2和IL-6的水平。[结论]铁皮石斛及其冻干粉对环磷酰胺引起的免疫抑制具有明显的改善作用，且后者药效更好。关键词铁皮石斛;冻干粉;环磷酰胺;细胞因子;免疫调节R285.5A0517-6611（2019）24-0180-02doi：10.3969/j.issn.0517-6611.2019.24.054StudyontheDifferenceofImmunomodulatoryEffectsofDendrobiumofficinaleandItsLyophilizedPoweronImmunosuppressedMiceZHANG激ng1，2，3，4，ZHUYa-qin1，2，4，ZHUYu-long1，2，4（1.SchoolofPharmacy，AnhuiUniversityofChineseMedicine，Hefei，Anhui230012;2.AnhuiProvinceKeyLaboratoryofChineseMedicineFormula，Hefei，Anhui230012;3.InstituteofPharmaceutics，AnhuiAcademyofChineseMedicine，Hefei，Anhui230012;4.SynergeticInnovationCenterofAnhuiAuthenticChineseMedicineQualityImprovement，Hefei，Anhui230012）Abstract[Objective]TheresearchaimedtostudythedifferenceinimmunomodulatoryeffectsofDendrobiumofficinaleanditslyophilizedpoweronimmunosuppressedmice.[Method]120SPFKunmingmalemiceweredividedintocontrolgroup，cyclophosphamidemodlegroup，Dendrobiumofficinaleanditslyophilizedpowergroup.Dendrobiumofficinaleanditslyophilizedpowergroupswerecontinuouslyadministratedfor30days.At24hoursbeforetheendoftheexperiment，themodelgroup，Dendrobiumofficinaleanditslyophilizedpowergroupwereintraperitoneallyinjectedwith200mg/kgcyclophosphamide.Attheendoftheexperiment，thethymusindex，spleenindex，DTH，andserumcytokinesIFN-γ，IL-2andIL-6weremeasured.[Result]Dendrobiumofficinaleanditslyophilizedpowerincreasedthethymusindex，spleenindexandtoeswelling，up-regulatedthelevelsofIFN-γ，IL-2andIL-6intheserumofimmunosuppressedmice.[Conclusion]Dendrobiumofficinaleanditslyophilizedpowerhasasignificantimprovementontheimmunosuppressioncausedbycyclophosphamide，andthelatterisbetter.KeywordsDendrobiumofficinale;Lyophilizedpower;Cyclophosphamide;Cytokine;Immunoregulation基金項目安徽省科技重大专项（17030801015）。作者简介张静（1996—），女，安徽宿州人，硕士研究生，研究方向：药剂学。-全文完-