基于网络药理学探讨血府逐瘀汤治疗冠心病的作用机制叶嘉豪胡志希钟森杰杨梦姚涛邱宏熊霞军摘要目的：分析血府逐瘀湯治疗冠心病的作用机制。方法：借助中药系统药理学数据库与分析平台（TCMSP）检索血府逐瘀汤的化学成分和靶基因，利用GeneCards获得与冠心病相关的靶基因，采用Cytoscape3.7.2绘制“中药成分-疾病靶基因”网络图，采用String数据库构建蛋白质-蛋白质相互作用（PPI）网络图，将PPI网络图导入Cytoscape3.7.2，借助CytoNCA插件进行网络拓扑分析，采用R643.6.1软件对靶基因进行GO功能和KEGG通路富集分析。结果：筛选出血府逐瘀汤相关化合物224个，作用于冠心病的相关靶点3564个，PPI网络显示Degree值排名前10位的靶基因依次为IL6、AKT1、ALB、VEGFA、CCL2、EGF、IL1B、MMP-9、CASP3、MAPK1，拓扑分析结果显示Degree值排名前10位的靶基因依次为PTGS2、MMP2、VEGFA、AKT1、ALB、CASP3、STAT3、IL6、MMP9、SERPINE1。GO富集分析预测出此方对冠心病的治疗机制可能与调控营养水平反应、对氧含量的反应、膜筏、膜微域、磷酸酶结合等通路相关。KEGG富集分析预测出此方治疗冠心病可能是通过糖尿病并发症AGE-RAGE信号通路、流体剪切应力和动脉粥样硬化、HIF-1信号通路、TNF信号通路等几个关键的通路达到治疗效果。结论：本研究探讨了血府逐瘀汤治疗冠心病的活性成分及其作用机制，血府逐瘀汤可通过多靶点、多通路治疗冠心病，为进一步研究该方作用机制提供了理论依据。关键词冠心病;血府逐瘀汤;网络药理学;靶基因;通路;作用机制AbstractObjective：ToanalyzethemechanismofXuefuZhuyuDecoctioninthetreatmentofcoronaryheartdisease.Methods：GeneCardswasusedtoobtaintargetgenesrelatedtocoronaryheartdisease，Cytoscape3.7.2wasusedtodrawanetworkdiagramof“Chinesemedicinecomponents-diseasetargetgenes”，StringdatabasewasusedtoconstructaPPInetworkdiagram，thePPInetworkdiagramwasimportedintoCytoscape3.7.2，andCytoNCAplug-inwasusedfornetworktopologyanalysis.R643.6.1wasusedsoftwareforGOfunctionandKEGGpathwayenrichmentanalysisoftargetgenes.Results：Atotalof224relatedcompoundsofXuefuZhuyuDecoctionwerescreened，and3564relatedtargetsforcoronaryheartdiseasewerescreened.ThePPInetworkshowedthatthetop10targetgeneswithdegreevaluewereIL6，AKT1，ALB，VEGFA，CCL2，EGF，IL1B，MMP-9，CASP3，MAPK1，topologicalanalysisresultsshowedthatthetop10targetgeneswithdegreevaluewerePTGS2，MMP2，VEGFA，AKT1，ALB，CASP3，STAT3，IL6，MMP9，SERPINE1.GOenrichmentanalysispredictedthatthetreatmentmechanismofthisformulaforcoronaryheartdiseasemayberelatedtotheregulationofnutritionallevelresponse，responsetooxygencontent，membranerafts，membranemicrodomains，phosphatasebindingandotherpathways.KEGGenrichmentanalysispredictedthatthetreatmentofcoronaryheartdiseasebythisprescriptionmayachievethecuratieeffectsthroughseveralkeypathwayssuchastheAGE-RAGEsignalingpathway，fluidshearstressandatherosclerosis，HIF-1signalingpathway，andTNFsignalingpathway.effect.Conclusion：ThisstudyexplorestheactivecomponentsofXuefuZhuyuDecoctiononcoronaryheartdiseaseanditsmechanismofaction.XuefuZhuyuDecoctiontreatscoronaryheartdiseasethroughmultipletargetsandmultiplepathways，whichprovidesatheoreticalbasisforfurtherresearchonthemechanismofactionoftheprescription.KeywordsCoronaryheartdisease;XuefuZhuyuDecotion;Networkpharmacology;Targetgenes;Pathways;Mechanism：R289.5;R541：Adoi：10.3969/j.issn.1673-7202.2021.09.009冠心病是由于冠状动脉粥样硬化病变[1]，导致血管狹窄或阻塞而引起心肌组织缺血、坏死。中医将其归类为“胸痹”“真心痛”等范畴，是以膻中或左胸部发作性憋闷、疼痛为主要临床表现的一种病证。根据《中国心血管病报告2018》统计[2]，我国目前估计心血管病现患病总人数为2....