藿香正气制剂中和厚朴酚及总多糖快速测定分析朱俊平梁慧慧文雯毛妍范诗琪刘文龙贺福元张喜利摘要：目的建立HPLC雙波长指纹图谱快速测定藿香正气制剂中和厚朴酚含量的方法，建立糖量计法快速测定其总多糖含量的方法，并分析其差异性。方法HPLC采用XB-C18（HS）色谱柱（4.6mm×250mm，5μm），乙腈和0.1%磷酸水溶液等度洗脱，流速1.0mL/min，进样量20μL，检测波长294、270nm，柱温30℃;糖量计法以50%乙醇为阴性对照;聚类分析重复性样品，对比不同指标间的聚类结果。结果HPLC测得和厚朴酚在1.40～0.14mg/mL范围内与峰面积线性关系良好，线性方程为A=12182633C-217418，r?=0.9995;藿香正气水中和厚朴酚浓度为（0.4008±0.0050）mg/mL，藿香正气滴丸中其浓度为（0.4879±0.0089）mg/mL。总多糖在0.91～0.29g/mL范围内与其百分值线性关系良好，线性方程为Y=51.949X+0.868，r2=0.9999;藿香正气水中总多糖浓度为（0.31±0.003）g/mL，藿香正气滴丸中其浓度为（1.12±0.005）g/mL。聚类分析筛选出和厚朴酚峰面积和总多糖百分值作为表现藿香正气水和藿香正气滴丸间差异的指标。结论本研究建立的HPLC及糖量计法能有效用于藿香正气制剂多类指标的质量控制及不同剂型间的差异考察;聚类分析筛选出的峰面积和总多糖百分值作为评价指标具有代表性和准确性。关键词：藿香正气水;藿香正气滴丸;高效液相色谱法;和厚朴酚;总多糖;快速测定;聚类分析：R284.1：A：1005-5304（2019）05-0078-05DOI：10.3969/j.issn.1005-5304.2019.05.017开放科学（资源服务）标识码（OSID）：Abstract：ObjectiveToestablishamethodforrapiddeterminationofhonokiolinHuoxiangZhengqiPreparationsbyHPLCdual-wavelengthfingerprint，andtoestablishasugarmeterforrapiddeterminationoftotalpolysaccharideinHuoxiangZhengqiPreparations，andtoanalyzeitsdifferences.MethodsHPLCwasperformedonanXB-C18（HS）column（4.6mm×250mm，5μm），withacetonitrileand0.1%phosphoricacidaqueoussolutionforisocraticelution.Theflowratewas1.0mL/min;theinjectionvolumewas20μL;thedetectionwavelengthwas294and270nm;thecolumntemperaturewas30℃.Sugarmetermethodwasperformedwith50%ethanolasnegativecontrol.Clusteranalysiswasconductedtoanalyzerepeatedsamplesandcomparisonofclusteringresultsamongdifferentindexes.ResultsThelinearrelationshipbetweentheconcentrationofhonokiolandthepeakareaintherangeof1.40–0.14mg/mLwasgoodbyHPLC.ThelinearequationwasA=12182633C-217418，r2=0.9995.TheconcentrationofhonokiolinHuoxiangZhengqiLiquidwas（0.4008±0.0050）mg/mL;theconcentrationofhonokiolinHuoxiangZhengqiDroppingPillswas（0.4879±0.0089）mg/mL.Thelinearrelationshipbetweentheconcentrationoftotalpolysaccharideanditspercentagewasgoodintherangeof0.91–0.29g/mL;thelinearequationwasY=51.949X+0.868，r2=0.9999.TheconcentrationoftotalpolysaccharideinHuoxiangZhengqiLiquidwas（0.31±0.003）g/mL;thetotalpolysaccharideinHuoxiangZhengqiDroppingPillswas（1.12±0.005）g/mL.TheareaofhonokiolpeakandthepercentageoftotalpolysaccharideasindicatorsofHuoxiangZhengqiLiquidandHuoxiangZhengqiDroppingPillsshowedsignificantdifferencesbetweendifferentformulations.ConclusionEstablishedHPLCandsugarmetermethodcanbeeffectivelyusedforthequalitycontrolandinvestigationofdifferencesbetweendifferentdosageformsofHuoxiangZhengqiPreparations;theselectedpeakareaandtotalpolysaccharidepercentageastheevaluationindexarerepresentativeandaccurate.Keywords：HuoxiangZhengqiLiquid;HuoxiangZhengqiDroppingPills;HPLC;honokiol;totalpolysaccharide;rapiddetermination;clusteranalysis藿香正气处方出自北宋《太平惠民和剂局方》，由苍术、姜制厚朴、陈皮、白芷、茯苓、大腹皮、生半夏、甘草浸膏、广藿香油、紫苏叶油组成，具有解表化湿、理气和中功效，用于外感风寒、...