动物科学技术学院：吴军摘要本文运用组织学H.E染色法、改良甲苯胺蓝染色法、醛品红—阿利新蓝染色法和阿利新蓝—高碘酸雪夫氏反应染色法对罗非鱼消化道肥大细胞的形态分布等进行研究。结果发现罗非鱼消化道中存在大量的肥大细胞。肥大细胞大小不一，呈圆形、椭圆形、长梭形、鼓槌形、锥体形或不规则形状，有的可见长突起，细胞核蓝染。肥大细胞主要分布在胃黏膜固有层近黏膜肌层处，粘膜层胃底腺及黏膜肌等结构。体积较小，形态不规则，胞核清晰，胞质内颗粒少。H.E染色，肥大细胞圆或卵圆形，常成群聚集在小血管旁，胞质内充满粗大的嗜碱性颗粒。改良甲苯胺蓝染色，也可显示出一些肥大细胞，胞质颗粒着紫红色。醛品红—阿利新蓝染色，胃肠道的肥大细胞细胞质颗粒呈紫红色至深紫色，弹力纤维也呈深紫色，红细胞呈鲜橙黄色，其余组织为淡黄色。阿利新蓝—高碘酸雪夫氏反应染色，肥大细胞大多数红染，说明胃肠道中含有较多的含中性黏多糖的肥大细胞。关键词：消化道肥大细胞HistochemicalStudyonHeterogeneityofMastcellsinthetilapiadigestivetractAbstractThedigestivetractandtheformsanddistributionofthemastcellsinthetilapiawereobservedbytisssuing,HEstaining,improvedtoluidinebluestaining，aldehydefuchsin-alcianbluestainingandalcianblue-xuefushiperiodatereactionstaininginthispaper.Theresultshowedthepresenceofmassivemastcellsindigestivetractoftilapia,theywereindifferentsizesandcouldberound,oval,Longspindle-shaped,drumstick-shaped,cone-shapedorirregularshaped,somemayhadlongprocessandblue-stainednucleus.DigestiveTractmastcellsmainlydistributeinthegastriclaminaproprianearthemuscularismucosa,mucosallayerofthefundicglandmucosamusclestructure.H.Estaining,themastcellscircleorroundcircular,oftengathernearbytheminutevessel,andwithinthecytoplasmtherewerefilledwithcoarsebasophilicgranules.Theimprovedtoluidinebluestaining,alsodemonstratesomemastcells,andcytoplasmicgranulesintopurple.Thealdehydefuchsin-alcianbluestaining,cytoplasmicgranulesofmastcellsofthegastrointestinaltractintopurpletodeeppurple,theelasticfibersalsoassumedintodeeppurple,otherorganizationsforfaintintoyelloalcianblue-xuefushiperiodatereactionstaining,themastcellintored,explainedthatinthegastro-intestinaltractthereweremanypolysaccharideincludingtheneutralitymastcells.Keywords:digestivetractMastcell目录一、文献综述....................................................11消化道研究概况..............................................11.1上消化道的功能...........................................11.2下消化道的功能...........................................21.3消化形式.................................................31.4食物的消化...............................................31.5食物的吸收...............................................41.6消化系统活动的调节.......................................52动物肥大细胞的研究慨况......................................7二、实验部分....................................................91前言........................................................92材料与方法..................................................92.1取材与固定.............................................102.2切片的制作.............................................102.3H.E染色...............................................102.4甲苯胺蓝改良染色.......................................102.5醛品红—阿利新兰法染色.................................112.6阿利新蓝—高碘酸雪夫氏反应（AB—PAS）染色..............113结果.......................................................113.1消化道胃、肠的结构......................................
