November2013Vol.33No.11文章编号：1001-6325(2013)11-1435-05研究论文以a甘露聚糖肽为佐剂的EV71全病毒灭活抗原黏膜免疫小鼠的抗体应答荆晶I。孙强九冯素玲2,赵兴华2,摩怡然2,王冰2•，黎卫平卜(1.兰州大学基础医学院病理研究所，甘肃兰州730000;2.陕西省新型治疗性疫苗工程技术研究中心，陕西西安710061)摘要：目的肠道71型全病毒灭活抗原(EV71Ag)联合a-甘露聚糖肽(PA)佐剂滴鼻免疫，观察金身免疫应答及持续时间。方法5~6周龄BALB/c雌性小鼠随机分为阴性对照组，阳性对照组，高剂鼠疫苗组，低剂量疫苗佐剂组和高剂量:疫苗佐剂组，每组10只。阴性对照组以PBS滴鼻，阳性对照组以EV71Ag肌注给药，免疫税以EV71Ag单独和不同剂量分别配伍PA滴鼻给药。首次给药后第0、2、3、4、5和6周采集血清及鼻肺灌洗液,ELISA法检测血清特异性IgG抗体水平及鼻肺灌洗液slgA抗体水平,M1T法检测淋巴细胞增殖，中和抗体实验检测小鼠血清中和抗体水平。结果各滴鼻组小鼠血清中均产生一定水平的特异性IgG抗体，高剂量疫苗佐剂组第三次免疫后其血清IgG(2.477±0.500)可达到阳性对照组(3.040±0.120)的80%,佐剂组体外淋巴细胞增殖率较阳性对照组有显著增高(P<0.05),各滴鼻组血清中和抗体滴度均>8。结论EV71全病毒灭活联合a-甘露聚糖肽佐剂免疫小鼠可诱导全身免疫应答,且a-甘露聚糖肽佐剂可增强其作用。关键词：黏膜免疫;EV71；中和抗体;特异性IgG抗体;slgA抗体;淋巴细胞增殖率中图分类号：R392.7；R373.2*3文献标志玛：AImmunizationresponseofenterovirus71wholevirusinactivationantigencombinedwithPAadjuvantbynasalmucosainmiceJINGJingU,SUNQiang2,FENGSu-ling2,ZHAOXing-hua2,LIAOYi-ran2,WANGBing2-,UWei-ping,"(1.Dept,ofPathology.SchoolofBasicMedicalSciencestLanzhouUniversity,Lanzhou730000；2.TherapeuticVaccinesEngineeringCenterofShanxiProvince.Xi'an710061.China)Abstract:ObjectiveTostudythesystemicimmuneresponseofanimalsimmunizedbytype71virusantigen(EV71Ag)combinedwithalphamannanpeptide(PA)adjuvant.MethodsFortyBALB/cmiceoffivetosixweeksoldwererandomlydividedintofivegroups.ImmunegroupsacceptedEV71Agwithorwithoutadjuvants.Bloodserumandbronchoalveolarlavageswerecollectedat0,2,3,4,5and6weekssincethefirstimmunetreatment.ThelevelofIgG,IgAandneutralizingantibodyinserumwasdetectedbyenzyme-linkedimmunosorbentassay(ELISA)andNeutralizingantibodytestsrepectively.MTTassaywasinvolvedtoassesstheproliferationoflympho-cyte.ResultsThespecificIgGantibodyinserumwasdetectedinallgroups.Therewasnostatisticallysignificantdifferentbetweenthehigh-doseadjuvantedgroupandEV71Agintramuscularinjectinggroup(2.477±0.500vs3.040±0.120).EV71Agcombinedwithalphamannanpeptide(PA)adjuvantcausedlymphocyteproliferationin收稿日期：20B-01-15修回日期：20B-03-28基金项目：国家高技术研究发展计划(863计划)(2012AA02A406),通信作$(correspondingauthor)：liwp@Izu.edu.cn;wangbing@mail.xjtu.cdu.cn---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---vitro,neutralizingantibodytiterincreasedinallgroups.Therewerenosignificantdifferencebetweenhigh-doseadju-vantedgroupandtheEV71Agintramuscularinjuectinggroup.ConclusionsEV71Agcombinedwithalphaman-nanpeptide(PA)adjuvantintranasallyinducedaneffectivesystemicimmunereponse.Keywords：mucosalimmunity；enterovirus71antigen；neutralizingantibody;specificIgGantibody;slgAantibody;lymphocyteproliferation人类肠道病毒71型（humanenterovirus?1,EV71）属小RNA病毒科肠道病毒，是手足口病的主要病原体之-，可导致手足口病、疱疹性咽峡炎以及中枢神经系统病变，手足口病已成为中国威胁学龄前儿童生命健康的一种新型疾病⑴。甘露聚糖肽又名多抗甲素（poluactinA,PA）,是我国西安交通大学医学院自主研发的国内首创的生物反应修饰物。PA由不同...