清脑镇痛液镇痛作用的实验研究[摘要]目的：观察清脑镇痛液连续5d经口灌胃给予小鼠后，所产生的镇痛作用。方法：试验选用SPF级昆明种小鼠120只，随机分为供试品高、中、低剂量组，阳性对照组和阴性对照组，每组12只，雌雄各半，采用压痛法（n=60）和醋酸扭体法（n=60），以痛阈提高率、扭体时间及扭体次数为指标，清脑镇痛液按25g生药/kg或12.5g生药/kg体重连续5d经口灌胃给予小鼠，研究清脑镇痛液对机械刺激和化学刺激引起疼痛的镇痛作用。结果：供试品高剂量组及阳性对照组小鼠的痛阈提高率明显提高，分别与阴性对照组比较差异均有高度统计学意义（P＜0.01），供试品中、低剂量组小鼠的痛阈提高率分别与阴性对照组比较差异均无统计学意义。供试品高、中、低剂量组及阳性对照组小鼠的初次扭体时间分别与阴性对照组比较差异均无统计学意义。供试品高、中剂量组及阳性对照组小鼠的扭体次数明显减少，分别与阴性对照组比较差异均有统计学意义（P＜0.01），供试品低剂量组小鼠的扭体次数与阴性对照组比较差异无统计学意义。结论：清脑镇痛液能显著提高小鼠痛阈提高率，减少扭体次数，有一定的镇痛作用。[关键词]清脑镇痛液；痛阈；扭体；镇痛[]R277.772[文献标识码]A[]1673-7210（2011）07（b）-033-02EmpiricalstudyonanalgesiceffectofQingnaoZhentongLiquidWUGangyi,LIUZhi,LIXiao激,LIDe′ai*QingdaoMunicipalHospital,ShandongProvince,Qingdao266011,China[Abstract]Objective:ToobserveQingnaoZhentongLiquid(QZL)throughthemouthfor5straightdays,afterfillinginmicestomachgivetheanalgesicactiongenerated.Methods:120SPFKunmingmicewererandomlydividedintotheproducthigh,mediumandlowdosegroup,positiveandnegativecontrolgroup,eachgroup12only,maleandfemalehalfandhalf,themethodoftenderness(n=60),aceticacidbodytorsion(n=60)accordingtoimproverateofpainthreshold,timeofbodytorsionandbodytorsionastheindex.QZLwasgivenaccordingto25gliquidcrude/kgor12.5gcrude/kgweightthroughthemouthfor5straightdaystomice,theanalgesiceffectofQingnaoZhentongLiquidwasresearchedonmechanicalstimuliandchemicalstimulationtheanalgesicactionwhichcausedpain.Results:Theproducthighdosegroupandpositivecontrolgroupcouldobviouslyimprovetheimproverateofpainthresholdandcomparedwithnegativecontrolgrouprespectively,thereweresignificantdifferenceinthem(P<0.01),theimproverateofpainthresholdintheproductoflow,mediumdosegroupwhichcomparedwithnegativecontrolgrouprespectively,therewasnosignificantdifference.Therewerenosignificantdifferencesonthefirsttimeofbodytorsionoftheproductofhigh,mediumandlowdosegroupandpositivecontrolgroupwhichcomparedrespectivelywithnegativecontrolgroup.Thebodytorsionintheproductofhigh,mediumdosegroupwerediminished,whichwerecomparedwithnegativecontrolgroupandthereweresignificantdifferencesinthem(P<0.01).Therewerenosignificantdifferencesonbodytorsionoftheproductoflowdosegroupcomparedwithnegativecontrolgroup.Conclusion:QZLcanobservablyincreasetheimproveratofpainthreshold,andreducetheofbodytorsion,soithasanalgesiceffect.[Keywords]QingnaoZhentongLiquid;Painthreshold;Bodytorsion;Analgesia清脑镇痛液是由桃仁、红花、当归、川芎等组成的中药复方制剂，具有活血通络、滋肾平肝之功。临床主要用于治疗血管性头痛（又称偏头痛）[1]。本文通过观察清脑镇痛液连续5d经口灌胃给予小鼠后，所产生的镇痛作用，以探讨清脑镇痛液治疗血管性头痛的有效性。1材料与方法1.1材料1.1.1药品清脑镇痛液，青岛市市立医院中药制剂室提供，每支10ml，每毫升含生药2.5g，批号：20090828；天舒胶囊，江苏康缘药业股份有限公司提供，每粒0.34g，批号：081207。1.1.2动物健康SPF级昆明种小鼠120只，雌、雄各半，体重18~22g，由青岛市实验动物和动物实验中心提供。动物检测报告编号为D20090003。本文为...