ChinJCancerBiother,Aug.2016，Vol.23,No.4DOI：10.3872/j.issn.1007-385X.2016.04.012•临床研宄•0CT4与EMT相关因子在浸润性乳腺癌组织屮的表达及其临床意义abaaa任静文，梁迪，赵连梅，单保恩(河北医科大学第四医院a.科研中心；b.检验科，张超，河北石家庄050011)［摘mesenchymaltransition,EMT)相关因子E-cadherin)和波形蛋要］目的：观察上皮间质转化(epithelial-弼黏着蛋0(E-bindingtranscriptionfactor4,0CT4)在浸润性乳腺癌中的表达情白(vimcntin)以及干细胞因子八聚体结合转荣因子4(octamer-况及0CT4与E-弼黏着蛋白和波形蛋白的相关性。方法：收集河北医科大学第四医院2009年手术切除的浸润性乳腺癌组E-织标本40例，实时荧光定量PCR和免疫组织化学法分别检测OCT4、钙黏着蛋白和波形蛋白的mRNA和蛋白的表达，分析OCT4和EMT相关蛋白之间的相关性以及与浸润性乳腺癌患者生存率之间的关系。结果：在浸其与临床病理特征的关系、0CT4、E-55%(22/40)和65%(26/40)。0CT4表达与润性乳腺癌组织中,钙黏着蛋白和波形蛋白表达率分别为30%(12/40)、2的表达有关，E-浸润性乳腺癌患者的年龄、组织学分级、淋巴结转移以及Her-钙黏着蛋0表达与淋巴结---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---转移有关，波形蛋白表达与组织学分级和淋巴结转移有关。0CT4mRNA和蛋白的表达与E-钙黏着蛋白和mRNA蛋白的表达呈负相关，与波形蛋白mRNA和蛋白的表达呆正和关，而E-钙黏着蛋白mRNA和蛋白的表达与波形蛋白mRNA和蛋0的表达也呈负相关。0CT4表达阳性患者的生存率明显低于表达阴性患者，差异具有统计学意义(P<0.05)；而E-钙黏着蛋白和波形蛋白的表达与患者可能是影生存率之间未见明显相关性(P〉0.05)。结论：干细胞因子0CT4可能通过EMT促进浸润性乳腺癌细胞的转移，响浸润性乳腺癌预后的因素。［关键词］浸润性乳腺癌；八聚体结合转录因子4;上皮间质转化；淋巴结转移［中图分类号］R737.9；R730.2［文献标识码］A［385X(2016)04-0525-06文章编号］1007-ExprcssionsofOCT4andEMT-associatcdfactorininvasionbrcastcanccrandtheirclinicalsignificancesZHANGChaoa,RENJingwcnb,LIANGDia，ZIIAOLianmcia,SIIANBaocna(a.ScientificResearchCentre；b.Depart-mentofLaboratory,FourthHospitalAffiliatcdtoIIcbciMcdicalUnivcrsity,Shijiazhuang050011，Hebei,China)[Abstract]Objective：TodetectexpressionsofmRNAsandproteinsforEMT-assosiatcdfactors,E-cadhcrinandvimen-1in,andstcmccllfactorOCT4ininvasivebreastcancerandcorrelationofOCTdwithE-cadherinandvimentin.Method：Fourtysamplcsofinvasivcbrcastcanccrtissucswcrccollcctcd.ExpressionsofmRNAsandproteinsforEMT-assosiatedfactors,E—cadherinandvimentin,andstcmccllfactorOCT4wcrcrcspcctivclydctcctcdbyRcal----本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---timcquantitativcPCRandiminunehistochemistryassay.RelationshipbetweenexpressionsofthemRNAandthcprotcins,andsurvivalratcsandclinicalpathologicalfcaturcsofthepatientswithinvasivebreastcancer,andcorrelationofOCT4withE-cadherinandvim-cntin,wereanalyzed.Rcsults：Inthcinvasivcbrcastcanccrtissues，expressionratesofOCT4,E-cadherinandvimentinwere30%(12/40),55%(22/40)，and65%(26/40)respectively.ThccxprcssionofOCT4wascorrclatedwithage,histologicalgrade,lymphnodemetastasisandHer-2expressionofthcpaticntswithinvasivcbrcastcanccr.ExpressionofE-cadhcrinwasrclatedwithlymphnodemetastasisandexpressionofvimentinwasrelatedwithhistologicalgradeandlymphnodemetastasis.ExpressionsofmRNAandprotcinforOCT4andE-cadherinwerenegativelycorrelated.Expres-sionsofmRNAandproteinforOCT4andvimcntinwcrcpositivcl5rcorrclatcd.A1soexpressionsofmRNAandproteinforE-cadherinandvimentinwerenegativelycorrelated.SurvivalratesofthcpaticntswithpositivccxprcssionofOCT4wcrcsig...