大孔吸附树脂分离纯化牛蒡叶绿原酸的工艺研究袁晓庆李莉刘言娟王振摘要[目的]建立大孔吸附樹脂分离纯化牛蒡叶中绿原酸的工艺。[方法]通过单因素试验研究提取液种类、浓度、pH、提取温度、料液比以及提取时间等参数对绿原酸提取率的影响，确定最佳提取工艺;以大孔吸附树脂对牛蒡叶中绿原酸的分离效率为评价指标，通过静态和动态吸附/解吸附试验优化分离纯化工艺。[结果]pH=1的蒸馏水为提取溶剂，料液比1∶20（g∶mL）、提取温度80℃、回流1h时对牛蒡叶中绿原酸的提取效果最佳，平均提取率为1.82%;考察了6种大孔吸附树脂对牛蒡叶绿原酸的分离纯化性能，以吸附/解吸附性能为评价指标，确定了LX-218为最佳大孔吸附树脂。LX-218型MAR分离纯化牛蒡叶绿原酸的最佳工艺条件为：上样量为30BV（树脂床体积），上样浓度为0.7倍提取原液浓度（相当于原生药），上样液pH=3，以4BV/h流速吸附，5BVpH=5的60%乙醇以5BV/h的流速解吸附。在优化的工艺条件下，牛蒡叶绿原酸得率为84.41%，纯度为55.26%。[结论]LX-218型大孔吸附树脂对牛蒡叶绿原酸有较好的吸附容量和解吸附率，优化的生产工艺条件适用于牛蒡叶绿原酸的工业化生产。关键词牛蒡叶;大孔吸附树脂;绿原酸;分离纯化;工艺优化R284.2A0517-6611（2020）04-0168-07doi：10.3969/j.issn.0517-6611.2020.04.049开放科学（资源服务）标识码（OSID）：StudyontheTechnologyofSeparationandPurificationofChlorogenicAcidfromBurdockLeavesbyMacroporousAdsorptionResinYUANXiao-qing，LILi，LIUYan-juanetal（LinyiUniversity，Linyi，Shandong276000）Abstract[Objective]Toestablishtheoptimumtechnologicalconditionsfortheseparationandpurificationofchlorogenicacidfromburdockleavesbymacroporousadsorptionresin（MAR）.[Method]Theoptimumextractionprocesswasobtainedbystudyingtheeffectsofextractanttype，concentration，pH，extractiontemperature，ratioofsolid-liquidratioandextractiontimewithsinglefactorexperiment.Basedonseparationefficiencyofchlorogenicacidfromburdockleaves，theoptimalseparationandpurificationprocesseswereobtainedbystaticanddynamicadsorption/desorptionexperiments.[Result]Theoptimumextractionprocessasfollows：theextractantwasdistilledwaterwithpH=1，theratioofsolid-liquidratiowas1：20（g：mL），theextractiontemperaturewas80°C，therefluxtimewas1h.Theaverageextractionratewas1.82%withtheaboveextractionprocess.Theseparationandpurificationperformanceofsixkindsofmacroporousadsorptionresinsforburdockchlorogenicacidwereinvestigated.Takingadsorption/desorptionperformanceastheevaluationindex，LX-218wasdeterminedasthebestmacroporousadsorptionresin.TheoptimalprocessconditionsonLX-218MARfortheseparationandpurificationofburdockchlorophyllicacidasfollows：theloadingamountwas30BV（resinbedvolume），theloadingconcentrationwas0.7timestheconcentrationoftheextractedstocksolution（equivalenttorawmaterial），andtheloadingsolutionpH=3，adsorptionat4BV/hflowrate，respectively.Forthedesorptionprocess，chlorogenicacidloadedMARcolumnwasdesorbedby5BVethanol-aqueoussolution（60∶40，V∶V）withpHvalueof5atflowrateof5BV/h.Undertheoptimalconditions，theyieldofchlorogenicacidfromburdockleavesandpuritywere84.41%and55.26%，respectively.[Conclusion]LX-218macroporousadsorptionresinhasgoodadsorptionanddesorptionperformanceforchlorogenicacid.Theoptimizedtechniqueissuitablefortheindustrialproductionofchlorogenicacidfromburdockleaves.KeywordsBurdockleaves;Macroporousadsorptionresin;Chlorogenicacid;Separationandpurification;Processoptimization牛蒡又名大力子、恶实、牛蒡子，属于菊科类植物，近年来作为保健型蔬菜受到人们的青睐。它全身是宝，其子、根均可入药，富含糖、纤维素、蛋白质、钙、铁等人体所需...