2009届毕业生毕业论文题目:植酸预处液对月季切花中SOD活性和MDA含量的影响院系名称:国际学院专业班级:生物制药0501学生姓名:张晏宁学号:200549701241指导教师:景红娟教师职称:讲师2009年6月21日2摘要在切花工业中,月季是当今世界上最重要的切花之一,它易种植,产量高,用途广泛,已成为全球花卉贸易中重要的商品。但是由于月季切花的自然寿命很低,如何延长其瓶插寿命及延缓衰老一直是人们研究的热点。现在虽然对月季采后保鲜的研究很多,却鲜有植酸应用于月季切花保鲜的报道。植酸作为一种天然存在的有机化合物,广泛存在于植物组织中,它含量丰富又容易提取,如果能够作为切花的保鲜剂将会有效降低切花工业的成本。本实验主要研究了两种浓度分别为0.7%与1.3%的植酸预处液对月季切花的保鲜效果,同时以蒸馏水为对照,每个处理重复三次。研究对象是一种名为“Shasha90号”的红色月季,通过测定切花的瓶插寿命,MDA含量、SOD活性等生理指标来判断植酸保鲜液对月季切花的影响。结果表明,0.7%与1.3%的植酸预处液均能够延有效地延缓MDA含量的增加,但1.3%的植酸保鲜液由于酸性过强对切花造成伤害而导致切花死亡。所以0.7%的植酸保鲜液的保鲜效果最好。关键词:植酸月季切花超氧化物歧化酶丙二醛ITitleEffectsofPretreatmentsContainedPhyticAcidonMDAContentandSODActivityofCutRoseFlowersAbstractInthecut-flowerindustry,roseiscurrentlytheworld'smostimportantcutflowersinoneeasycultivation,highyield,widerangeofuses,theglobalflowertradehasbecomeanimportantcommodity.However,duetothenaturallifeofcutroseflowersisverylow,howtoprolongtheirvaselifeandanti-agingresearchhasbeenahotspot.Althoughroseisnowonpost-harvestpreservationofmanyofthestudies,butrarelyusedinphyticreportsfreshcutrose.Phyticacidasanaturallyoccurringorganiccompounds,widelypresentinplanttissue,ithasrichcontentandeasytoextract,ifthefreshcutflowerscanbeusedasagentswillbeeffectivetoreducethecostofcutflowersindustry.Experimentalstudiesofthetwoconcentrationswere0.7%and1.3%ofthephyticacidpretreatmentonthepreservationoftheeffectofcutroseflowers,whiledistilledwaterascontrol,eachrepeatedthreetodealwith.Studyiscalled"Shasha90No."RedRose,bydeterminingthevaselifeofcutflowers,MDAcontent,SODactivityandotherphysiologicalindicatorstodeterminethefreshnessofphyticacidontheimpactofcutroseflowers.Theresultsshowedthat0.7%and1.3%solutionofphyticacidpretreatmentwereabletodelaytheextensionoftheeffectiveincreaseinMDAcontent,but1.3%solutionofphyticacidasaresultoftoomuchharmtocuttheleadtodeaths.Therefore,0.7%ofthephyticacidpreservationofthebestpreservationsolution.Keywords:PhyticAcidCutFlowerSODMDA目次1.绪论..………………………………………………………………………………….……..1II1.1影响月季切花保鲜的因子………………………………………………………….…..11.2月季切花的化学保鲜技术………………………………………………………….….21.3植酸的抗氧化功能与切花保鲜…………………………………………………….…31.4前景展望…………………………………………………………………………….…..41.5本次课题研究内容………………………………………………………………….….52.实验仪器、药品、材料及实验方法…………………………………………….……..52.1实验仪器…………………………………………………………………………….….52.2实验药品……………………………………………………………………….………….62.3实验材料………………………………………………………………………...………62.4实验处理………………………………………………………………………………….72.5实验溶液制备…………………………………………………………………………….72.6实验方法………………………………………………………...