沈阳农业大学学报袁圆园11原04袁42穴2雪184院-189允燥怎则燥灶枣葬杂造澡藻灶赠粤葬早灶则早蚤糟怎哉造灶贼蚤怎增则藻葬则造泽11蚤原04贼赠袁42袁穴圆2雪园184院-189几种外源物质对氧化葡萄糖酸杆菌生长及产2-酮基-L-古龙酸的影响吕淑霞1袁赵朔1袁马镝1袁林英1袁张良1袁陈宏权2袁张忠泽3渊1.沈阳农业大学生物科学技术学院,沈阳110161;2.东北制药总厂Vc公司,沈阳110028;3.中国科学院沈阳应用生态研究所,沈阳110016冤摘要院通过在发酵培养基中添加不同的外源营养物质及巨大芽孢杆菌的培养上清液袁研究其对氧化葡萄糖酸杆菌生长及产2-酮基-L-古龙酸(2-KGA)的影响遥结果表明袁添加适量的谷氨酸单钠(MSG)尧腺苷三磷酸(ATP)尧含氮碱基尧二氢叶酸和巨大芽孢杆菌的培养上清液都能够促进菌体增殖并提高2-KGA产量袁而且2-KGA产量与生物量呈正相关遥巨大芽孢杆菌的培养上清液促进菌体生长和产2-KGA的作用远大于以上各种外源营养物质遥添加培养36h的巨大芽孢杆菌胞外液后袁氧化葡萄糖酸杆菌的菌落数和2-KGA产量分别是对照的13.39倍和8.81倍袁而添加外源营养物质中促进作用最大的二氢叶酸后仅为对照的4.12倍和2.97倍遥这表明袁2-KGA产量的增加主要是通过促进氧化葡萄糖酸杆菌的菌体增殖实现袁巨大芽孢杆菌的培养上清液促进氧化葡萄糖酸杆菌生长和产酸的机制不同于其他外源营养物质袁可能是通过某种生物活性物质提高氧化葡萄糖酸杆菌菌群密度来实现遥关键词院氧化葡萄糖酸杆菌曰巨大芽孢杆菌曰2-酮基-L-古龙酸曰外源物质中图分类号院Q936文献标识码院A文章编号院1000-1700渊2011冤02-0184-06EffectofSeveralExogenousSubstancesonGrowthand2-Keto-L-GulonicAcidAccumulationofGluconobacteroxydans11111L譈Shu-xia,ZHAOShuo,MADi,LINYing,ZHANGLiang,23CHENHong-quan,ZHANGZhong-ze(1.CollegeofBiologicalScienceandTechnology,ShenyangAgriculturalUniversity,Shenyang110161,China;2.NortheastPharmaceuticalGeneralFactoryofVitaminCCompany,Shenyang110028,China;3.InstituteofAppliedEcology,AcademiaSinica,Shenyang110016,China)Abstract院Theeffectofseveralexogenousnutrientsubstancesonthegrowthand2-keto-L-gulonicacid(2-KGA)productionofGluconobacteroxydanswasstudied.Itwasfoundthatadditionsofappropriateamountofmonosodiumglutamate(MSG),adenosine5忆-triphosphate(ATP),nitrogenousbases,dihydrofolicacidandthesupernatantofBacillusmegateriumcouldpromotethecellproliferationand2-KGAproductionofG.oxydans,anditsbiomasswaspositivelycorrelatedwiththeproductionof2-KGA.ThegrowthpromotingeffectonG.oxydansbythesupernatantofB.megateriumwasmostsignificantamongtheexogenousnutrientsubstancesmentionedabove.AfteraddingthesupernatantofB.megateriumculturedfor36hinthefermentationmedium,thebiomassconcentrationand2-KGAproductionofG.oxydanswere13.39and8.81timeshigherthanthecontrol,respectively;whileafteraddingdihydrofolicacid,whichhadthegreatestpromotingeffectamongtheexogenoussubstances,thebiomassconcentrationand2-KGAproductionofG.oxydanswereonly4.12and2.97timeshigherthanthecontrol,respectively.Itsuggeststhatthehighlyaccumulationof2-KGAmightbeduetothevigorousgrowthofG.oxydans.ThemechanismisdifferentbetweenthesupernatantofB.megateriumandtheexogenoussubstancestothegrowthandthe2-KGAproductionofG.oxydans,andthesupernatantofB.megateriummightcontainsomebioactivesubstanceswhichincreasethemassdensityofG.oxydans.Keywords院Gluconobacteroxydans;Bacillusmegaterium;2-keto-L-gulonicacid;exogenoussubstances2-酮基-L-古龙酸(2-keto-L-gulonicacid,2-KGA)是维生素C(vitaminC,Vc)合成的前体遥我国工业生产Vc通常采用野二步发酵法冶袁即在野莱氏法冶第一步发酵的基础上袁再采用氧化葡萄糖酸杆菌(Gluconobateroxydans,G.oxydans)和巨大芽孢杆菌(Bacillusmegaterium,B.megaterium)混合菌发酵将L-山梨糖转化为2-K...