ChinesemedicinerecoverydrinkofperipheralbloodmononuclearcellsofpatientswithseveresepsisofTLR2andTLR4geneexpressionToobservetheimpactofChinesemedicinerecoverydrinkonperipheralbloodmononuclearcellsofpatientswithseveresepsisofTLR2,TLR4geneexpressiontowritepapersnetwork:[Abstract][Method]40patientswererandomlydividedintotherecoverydrinkgroup(treatmentgroupandtheplacebogroup(controlgroup,20casesinbothgroupswithWesternconventionalcaretreatmentwiththeuseoftraditionalChinesemedicinetreatmentgrouprecoverydrink(byworkersbezoar,Americanginseng,astragalus,rhubarb,gypsum,Ophiopogon,Salvia,TPGmonkeybezoarcontrolgroupplusplacebo.selected15healthysubjectsservedasnormalgroup.usingreversetranscription-polymerasechainreaction(RT-PCR)methodforthedeterminationbeforetreatmentandtreatmentof3and7daysinperipheralbloodmononuclearcellTLR2,TLR4mRNAexpressionchanges.[Results]Beforetreatment,thetwo---本文于网络，仅供参考，勿照抄，如有侵权请联系删除---groupsofpatientsmonocyteTLR2,TLR4mRNAexpressionwassignificantlylowerthanthatofthenormalgroup(P《0.05)thecontrolgroupinthefirst7daysoftreatmentTLR2,TLR4mRNAexpressioneachobservationpointafterthestartwassignificantlyhigher(P《0.01);treatmentgroupinthetreatmentofthefirstthreedaysofTLR2expressionofTLR4mRNAreboundedsignificantly,andtreatmentgeneexpressionwassignificantlyhigher(P《0.05);butthetwogroupsofTLR2,ofTLR4mRNAexpressiondidnotreachthelevelofthenormalgroup(P《0.05orP《0.01.[Conclusion]ChinesemedicinerecoverydrinksignificantlyincreasedTLR4,TLR2mRNAexpressioninperipheralbloodmononuclearcells,reducingmortalityofpatientswithseveresepsis.Keywords:]traditionalChinesemedicinetherapiesinsepsisrecoverydrinktherapeuticapplicationsToll-likereceptorSepsisisasystemicinfectiousdisease.Previousstudiesthatthecellwallcomponentsofavarietyofpathogenicmicroorganisms[suchasGram-negativebacteriaendotoxin(LPS,Gram-positivebacteria,---本文于网络，仅供参考，勿照抄，如有侵权请联系删除---peptidoglycan(PGN)andlipoteichoicacid(LTA)]canbecombinedbythecellsurfaceproteinreceptorCD14,inducedsystemicinflammatoryresponseleadingtosepticshockormultipleorgandysfunctionsyndrome(MODS.However,CD14itselfisamembrane-anchoredprotein(lackingthetransmembranedomainandtheintracellularregion,notdirectlytotheconductionLPSsignalingwithincells,recentresearchfoundthattheToll-likereceptors(Tolllikereceptors,TLRs)mightLPStransmembranesignaltransductiontheportalproteins[1].Currently,theclonedhumanTLRsmembers10(i.e.TLR1to10),whereintheroleofTLR2andTLR4particularlysignificant.TLRsmatchnaturalimmunityintheeye,themonitoringandidentificationofvariouspathogen-specificmolecularpatterns(pathogenspecificmolecularpatterns,PAMPs),activationofNFkappaBsignalingpathway,toinitiateaseriesofimmuneandinflammatoryresponse,andultimatelyachievethepurposeoftheclearinvasionofpathogenicmicroorganisms,innateimmuneplayanimportantanti----本文于网络，仅供参考，勿照抄，如有侵权请联系删除---infectiveimmunefunction.TLRssignalreceptortargeting,maybringnewhopeforthepreventionandtreatmentofsepsispaperbyobservingtraditionalChinesemedicinerecoverydrinkontheperipheralbloodmononuclearcellsofpatientswithseveresepsisofTLR2andTLR4geneexpressionfromreceptorangleexploretheroleoftraditionalChinesemedicineinanti-infectionimmunityresultsreportedbelo1MaterialsandMethods1.1Clinicaldata1.1.1Caseselectionandg...