ChitooligosaccharidesS180sarcomacellapoptosiscellcycleandapoptosis-relatedproteinBcl-2BaxinfluencePapersnetwork:[Keywords]chitooligosaccharides;S180sarcoma;Cellcycle;apoptosis;miceAbstract:ObjectiveToinvestigatethebiologicaleffectsofchitooligosaccharidesS180sarcomacells,toprovideanewtheoreticalbasisforitstreatmentoftumors.Methodsinvitro:flowcytometrymeasurementschitooligosaccharidesroleS180sarcomacellcycle,apoptosis,circumstancesvivo:S180sarcomamicesubcutaneousxenograftmodelgiventhechitooligosaccharidesmeasuredtumorvolume,andtumortissuemeasuredbyimmunohistochemistrymethodBax,Bcl-2proteinexpressionresultsvitro:thehighconcentrationofshelloligonucleotidesugarafterS180sarcomacellsarrestedinG0.G1Qi,theS180sarcomaatthesametimeincreasedapoptosis.vivo:chitooligosaccharidesS180sarcomainmicesignificantlyinhibitedandcanimproveandpromotetheexpressionofapoptoticgeneBaxreducesuppressionofapoptotic1geneBcl-2expression.conclusionchitooligosaccharidesinhibitthegrowthofS180sarcomacells,S180cellswerearrestedinG0GPhase1andinduceapoptosis,whichmayberelatedBcl-2proteinthereductionintheupregulationofBaxprotein.Keywords:chitooligosaccharides;S180sarcoma;cellcycle;apoptosis;miceAbstract:ObjectiveToinvestigatethebiologicaleffectofchito-oligosaccharideonSarcoma180(S180cellstoassessthepossibilityandpotentialvalueofchito-oligosaccharideintumortherapy.MethodsIntheexperimentinvitro,suspendedS180cellsweretreatedwithchito-oligosaccharide.Flowcytometrywasusedtostudythechangesintumorcellapoptosisandcellcycleconsequentialtothetreatment.Forexperimentinvivo,KunmingmicebearingS180ingroupsweregivenvarieddosesofchito-oligosaccharidefor7days.Themicewerethenkilledtodeterminethetumorvolumeandtodeterminetheexpressionofapoptosis-relatedproteins(Bcl-2andBaxbyimmunohistochemistry.ResultsExperimentinvitroshowedrelativelyhighconcentrationofchito-2oligosacharridearrestedthegrowthofS180cellsatG0.G1phaseandincreasedtheapoptoticrateoftumorcells.ExperimentinvivoshowedthegrowthofS180cellswasinhibitedbychito-oligosaccharide(inaconcentration-depen-dentmanner.TheexpressionofantiapoptoticproteinBcl-2wasdecreasedandtheexpressionofproapoptoticproteinBaxwasincreased.ConclusionChito-oligosaccharidecaninhibitthegrowthofS180cells,arrestingthematG0.G1-phaseandstim-ulatinganincreaseoftheirapoptosis,possiblythroughdownregulationofBcl-2andupregulationofBax.Keywords::chito-oligosaccharide;sarcoma180cell;cellcycle;apoptosis;miceTumorisoneofthemostimportantdiseasesofharmtohumanhealth.Currently,mostoftheanticancerdrugssuppressionanddestructionoftumorcells,butoftenalsoaffectnormalcellmetabolism,thusaffectingtheefficacyandtumorpatientsmoredebilitatingandevencausethepatient’sdeath.lookingforlowtoxicity,safe,efficientanticancerdrugshasalwaysbeenakeyresearchprojectontheinternationalmarinepolysaccharidechitin(natural3polymersubstancesextractedfromshrimpandcrabshellsasbiosynthesisIthasmanyuniqueadvantagesofbiodegradable,safe,non-toxic,goodbiocompatibilityandchemicallystablederivativeproductofdegradationofchitosan,chitindeacetylatedchitooligosaccharidesathomeandabroadforthechitosanextensiveresearch,alargenumberofstudiesalsofoundthatchitosananditsderivativescaninhibittumorgrowthbyactivatingtheimmunesystem[1].Chitosanisdifficulttodissolveinwaterbringsomedifficultiestothewidelyusedchitooligosaccharidesmanysimilarnatureandchitosan,haslowtoxicity,easilys...