痕实验模型后观察伤口愈合的情况、瘢痕愈合后的病理改放射治疗兔耳增生性瘢痕实验探究［摘要］目的：研究经过医用高能电子线照射兔耳增生性瘢变及肌动蛋白（ACTIN）和血管生长因子（VEGF）表达变化情况。方法：选用日本大耳白兔18只，在每只兔耳腹侧面制作直径6mm的圆形全层皮肤缺损创面10个。将总计360个缺损创面模型随机分为6组，第1组为空白对照组（3只），其余5组为照射组（各3只L将照射组每只兔耳随机用200cGy、400cGy、600cGy、800cGy、1000cGy的6Mev电子线照射兔耳缺损创面，照射野周围用铅板防护，观察兔耳缺损创面的愈合情况变化及创面愈合后瘢痕组织进展情况。并于致伤一个月后对实验模型取材，进行光镜、电镜及组织学观察结果：兔耳腹侧面圆形创面缺损，能产生与人类增生性瘢痕类似的增生块。各照射组，缺损创面愈合延迟。总照射剂量相近的情况下单次不同照射剂量对增生性瘢痕形成的影响有显著性差异。结论：日本大耳白兔可以形成类似人的增生性瘢痕病理改变，可以用作增生性瘢痕研究的实验动物模型。动物实验证明，医用6Mev高能电子线照射治疗是一种有效的治疗增生性瘫痕的方法，总照射剂量相近的情况下，分5次照射、单次剂量400cGy,为最佳照射剂量。［关键词］增生性瘢痕；放射治疗；胶原纤维；电子直线加速器；［中图分类号JR619+.6［文献标识码］A［文章编1008-6455(2012)01-0074-04Experimentalstudyofradiationtherapyforhypertrophicscarinrabbits'earsZHAOYue1,ZHANGRong-ming2,ZHONNGRui-jia3,HUANGChao4,XINGShuang5,WUCun-gang6,(1.StudyingforMaster'sDegree,TheFirstAffiliatedHospitalofLiaoningMedicalUniverisity,Jinzhou121000,Liaoning,China;2.DepartmentofPlasticSurgery,TheFirstAffiliatedHospitalofLiaoningMedicalUniverisity;3.LiaoningprovineeEYEHospital,Shenyang;4.DepartmentofStomatology,TheFirstAffiliatedHospitalofLiaoningMedicalUniverisity;5.DepartmentofUltrasound,TheThirdAffiliatedHospitalofLiaoningMedicalUniverisity;6.DepartmentofUltrasound,TheFirstAffiliatedHospitalofLiaoningMedicalUniverisity)Abstract:ObjectiveThestudywentthroughaftertheirradiationofhighenergyelectronicwireswhichwasconductedontheexperimentalmodelofhypertrophicscarofrabbits,ears.Observedthehealingprocessandshowedthepathologicalchangeaswellasthevariationofactin(ACTIN)andvascularendothelialgrowthfactor(VEGF)afterthehealingofthescar.MethodsSelected18Japaneserabbitsandmade10modelsofcircularfull-thicknesswounddefectsintheventralsideofperearwhicheachonewasinthesizeof6mmdiameter.Thetotalnumberof360wounddefectswasrandomlydividedinto6groups.Grouplwastheblankcontrolgroup(3samples),andtheother5groupsweretheradiationgroup(3samplesineachgroup).Eachwounddefectsonrabbits'earswasrandomlyirradiatedwith200cGy,400cGy,600cGy,800cGy,1000cGy6Mevelectronicwiresandtheradiationfieldaroundwasprotectedbythestereotype.Observedthechangeduringthehealingprocessandtheprogressofthescartissueafterthewounddefectshadalreadyhealed.Gottheexperimentalmaterial1monthafterthetreatmentinjuryandcontinuedontheobservationsofthelightmicroscopy,electronmicroscopyandhistology.ResultsThewounddefectsintheventralsideoftherabbitsJearscouldproducethesimilarhypertrophicscarlikehumanbeings・Eachradiationgroupshowedthatthehealingofthewounddefectswasdelayed.Underthesimilaramountoftheradiation,differentvolumeofradiationpertimecouldresultasignificantdiffereneeontheformationofthehypertrophicscar.ConclusionJapaneserabbits,couldproducethesimilarpathologicalchangeofhypertrophicscarlikehumanbeings,andtheycouldbetheexperimentalanimalmodelfortheobservationonpathologicalscar.Thisexperimentproofedthatradiationwith6Mevpathologicalelectronicwiresisaneffectiveway...