蝉拟青霉不同纯化组分对体外抗肿瘤作用基础探究摘要：目的：为了进一步研究蝉拟青霉总多糖和腺昔单用或分别联合顺钳(DDP)或阿霉素(ADM)在体外的抗肿瘤作用。方法:用MTT法检测DDP、ADM、总多糖、总腺昔单独及联合用药对人肺腺癌(PAA-1)细胞的生长抑制能力；用流式细胞仪检测其对体外培养细胞系细胞周期的影响；用光镜检查其对体外培养PAA-1细胞系集落形成率的影响；用流式细胞仪检测其对体外培养淋巴细胞亚群分化的影响。结果:DDP、ADM、总多糖、腺昔对人肺腺癌(PAA-1)细胞生长抑制的IC50分别为9.05ug/mL、36.21ug/mL>10.86mg/mL、7.81mg/mLoDDP或ADM和多糖联用对PAA-1细胞的生长抑制主要表现出拮抗效应；0.5mg/mL、0.25mg/mL腺昔与4ug/mL阿霉素联合作用时，显示出拮抗效应,而当8mg/mL腺昔与4ug/mL阿霉素联用时，显示协同效应；不同浓度腺昔单用与和DDP联合使用相比，对细胞生长影响无显著性差异;腺昔和ADM联合作用,随着腺昔浓度的增加,GO/G1期细胞逐渐减少，而G2/M期相对增加。对体外培养PAA-1细胞系集落形成率多糖组和阴性对照组未见明显差异，不同浓度腺昔组的集落形成率略低于阴性对照组，随着腺昔浓度逐渐增加，集落形成率逐渐下降。多糖对体外培养淋巴细胞亚群的分化无影响。结论：总多糖、腺昔具有一定的体外抗肿瘤作用，总多糖与DDP或ADM联用不能增加其体外的抗肿瘤作用，腺昔与ADM联用可增加其体外的抗肿瘤作用。关键词：蝉拟青霉；总多糖;腺昔;肿瘤；体外❷中图分类号:R285.5文献标识码：A文章编号：1673-7717(2010)04-0760-05ResearchofAnti-tumorEffectsofDifferentPurifiedComponentsofCordycepsCicadaeinVitroCAIJu❷fen,JIANGZhi^ming,LUHong^yang,LUBo❷zheng(ZhejiangCancerHospital,Hangzhou310022,Zhejiang,China)Abstract：❷Objective：❷Tofurtherstudyanti-tumoreffectinvitroofpolysaccharidesandadenosinerespectively,orcombinedwithcisplatin(DDP)oradriamycin(ADM)・❷Methods：❷Detectionofinhibitionofhumanlungadenocarcinoma(PAAT)cellgrowthwithDDP,ADM,polysaccharidesandadenosinerespectivelyorcombinedadministrationbyMTTassay,cellcyclebyf1owcytometryonculturedcelllines,colony-fonningrateofPAA-1cellinvitrobylightmicroscopeexamination,andlymphocytesubsetsbyf1owcytometry❷Results：❷IC50ofDDP,ADM,totalpolysaccharides,andadenosineongrowthinhibitionofhumanlungadenocarcinoma(PAA-1)cellwere9.05ug/mL,36.21Pg/mL,10❷86mg/mLand7.81mg/mL.ItisantagonistofPolysaccharidecombinedwithADMorDDPongrowthinhibitionofPAA-1cells・Adenosinewith8mg/mLcombinedwithADMwith4ug/mLindicatesynergisnItisnodifferentbetweendifferentconcentrationsofadenosinealoneandcombinedwithDDPoncellgrowth.Withtheincreaseofconcentrationofadenosine,G0/G1phasecellsdecreased,whiletheG2/MphaserelativeincreasewhenadenosinecombinedandADM.Itisnodifferentbetweenpolysaccharidegroupandnegativecontrolgrouponcolony-formingrateofPAA-1cellinvitro.Adenosinegroupswereslightlylowerthannegativecontrolgrouponcolony-formingrate・Withagradualincreaseintheconcentrationadenosineof,therateofcolony-forminggraduallydecreased・PolysaccharideshadnoeffectononlymphocytesubsetsofPAA-1cellinvitro.❷Conclusion：❷Thepolysaccharidesandadenosinehasacertainanti-tumoTeffectsinvitro.PolysaccharidecombinedwithADMorDDPcannotincreasetheiranti—tumoreffectsinvitro,adenosinecombinedwithADMcanincreaseanti-tumoreffectsinvitro•❷Keywords：Cordycepscicadae；totalpolysaccharides；adenosine；tumor；invitro收稿日期：2009-11-10基金项目:浙江省中医药管理局资助项目(2007CB186)作者简介：蔡菊芬(1953-),女，浙江萧山人，主任医师,研究方向：肿瘤化疗及中西医结合诊治。蝉花是一传统名贵中药，含有多种必须氨基酸、真菌多糖、腺昔、生物碱、麦角笛醇、虫草素酸等成分，具有滋补强壮，抗疲劳抗应激以及免疫调节作...