畜牧兽医学报2008,39(1):53259ActaVeterinariaetZootechnicaSinica鸭源致病性大肠杆菌的血清型鉴定及其相关毒力基因分析于学辉1,2,程安春1,33,汪铭书1,33,王英2,王远微2,汤承2(1.四川农业大学动物医学院禽病防治研究中心,雅安625014;2.西南民族大学生命科学与技术学院,成都610041;3.动物疫病与人类健康四川省重点实验室,雅安625014)摘要:自规模化养鸭场患典型大肠杆菌败血症雏鸭分离的282株致病性大肠杆菌(E1coli)210株(包含37种血清型),其中O93、O78、O92、O76占4318%(92/210)、O93混合型为首次从鸭群中分离到。应用PCR结合核酸序列测定对2101coli()和28株自健康雏鸭泄殖腔拭子分离的E1coli())中的鼠疫菌素受体基因(fyuA)和铁调节蛋白基因(irp2)、If)(papA)和血清耐受基因(iss)检测,结果表明:frp4110%、4313%、9219%带率分别为2114%、2510%、9219%、100%和9219%,iss、fimC和papA携带率差异不显著(P>0105),但papA的携带率(鸡、猪和人)源E1coli;HPI毒力岛在鸭源E1coli中分布较广,其携带率表现为鸭大肠杆菌病分离株极显著高于临床健康鸭大肠杆菌分离株。HPI毒力岛的携带率与菌株的致病性呈明显的正相关,与O78等特定的血清型有一定的关系。鸭大肠杆菌病分离株有3716%(79/210)同时携带fyuA、irp2、fimC、iss和papA基因,极显著高于健康鸭大肠杆菌分离株的1413%(4/28)(P<0101)。关键词:鸭;致病性大肠杆菌;血清型;毒力基因;分子流行病学:S852161+2文献标识码:A:036626964(2008)0120053207SerotypeIdentificationandVirulence2associatedGenesAnalysisofPathogenicE.coliIsolatedfromDucklingsYUXue2hui1,2,CHENGAn2chun1,33,WANGMing2shu1,33,WANGYing2,WANGYuan2wei2,TANGCheng2(1.AvianDiseaseResearchCenter,CollegeofVeterinaryMedicine,SichuanAgriculturalUniversity,Yaan625014,China;2.CollegeofLifeScienceandTechnology,SouthwestUniversityforNationalities,Chengdu610041,China;3.KeyLaboratoryofAnimalDiseaseandHumanHealthofSichuanProvince,Yaan625014,China)Abstract:Amongthe282E.colistrainsisolatedfromducklingstypicallyshowingE.coliseptice2miainlarge2scaleduckfarmsinSouthwestChina,theserotypesof210strainswereidentified.Theresultsshowedthattotally37serotypeswereobtained,ofwhichserotypesofO93,O78,O92andO76accountedfor43.8%andweretheprevalentserotypes,andserotypesofO46,O32O93,O60O93wereisolatedfromducksatthefirsttime.Thepesticinreceptorgene(fyuA)withinthehighlypathogenecityislands(HPI),ironregulatoryproteingene(irp),type收稿日期:2007208216基金项目:国家科技攻关重大项目(2004BA901A03);“”国家十一五科技支撑计划(2007Z062017);“”教育部新世纪优秀人才支持计划项目(NCET20420906/NCET20620818);四川省重大基础研究项目(04JY029200621);四川省重点建设学科项目(SZD0418)作者简介:于学辉(19612),男,四川绵阳人,博士生,主要从事禽病学研究工作3通讯作者:程安春,汪铭书,Tel:083522882918,Fax:083522885774,E2mail:chenganchun@vip11631com©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.wwcnki54畜牧兽医学报39卷1pilusessentialproteingene(fimC),thepappilusstructuralproteingene(papA)andserumtoleranceassociatedgene(iss)ofthe210pathogenicE.colistrainsisolatedfromducksshowingclinicalsignsandthe28E.colistrainsisolatedfromcloacalswabofclinicallyhealthyducklingsweredetectedbythemethodofpolymerasechainreaction(PCR)andnucleicacidsequenceanaly2sis.TheresultsdemonstratedthatthegenesoffyuA,irp2,fimC,papAandissweredetectedfrompathogenicE.colistrainsisolatedfromducksshowingclinicalsignsandaccountfor4110%,4313%,9219%,9716%and9617%respectively,andthecorrespondinggenesdetectedfromE.colistrainsisolatedfromclinicallyhealthyducklingsaccountfor2114%,2510%,9219%,100%and9219%respectively.Theresultsshowedthattherewerenosignificantdifference(P>0105)of...