临床医学自体骨髓间充质干细胞移植治疗阿尔茨海默病4例承李义召1巴王庆徳3,朱士文2,崔贵祥2,谢克勤&时庆-侯怀水役沈们钧4AutologousbonemesenchymalstemcelltransplantationforAlzheimer'sdiseasein4casesLiYi-zhao1,2,WangQing-de3,ZhuShi-wen2,CuiGui-xiang2,XieKe-qin2,ShiQing4,HouHuai-shui4,ShenBo-jun4Abstract:TenpatientswithAlzheimer^sdiseaseweredividedintostemcelltreatmentinsitugroup(?:=6),whichunderwentrecombinedgnmulocytecolony-stimulatingfactortreatment(G-CSFgroup),150Mg,onceperday.with7-10daysasonetreatmentcourse,combinedwithSalviamiltiorrhizaintravenousinjection(20mlVd)for10days;autologousbonemesenchymalstemcelltreatmentgroup(MSCs,门=4),inwhichbonemarrow(230330mL)washarvestedfromthepaticnts,iliaandthenthemononuclearcellswereisolatedbydensitygradientcentrifugation,andthemononuclearcellswereiinjectedintravenouslyorintosubarachnoidspacebylumberpunctureonceorseveraltimes.Inaddition.theG-CSFwassubcutaneouslyinjectedonthesameday(stemcelltreatmentinsitu).Beforeandafterstemcelltreatment,mini-mentalstateexamination(MMSE),AbilityofDailyI-iving(ADL)andperipheralbloodcellsweremeasured,andneuralfunctionandside-effectswereassessedrespectively.Therewerenoside-effectsintheG-CSFtreatedpatients,andperipheralbloodleucocytewascountedfor35-40x10°L\BeforeandthreemonthsafterG-CSFtreatment,theMMSEandADLscoresweresignificantlyimprovedcomparedwithbeforetreatment(P<0.05)respectively.TwocasesintheMSCsgrouphadfever(mentemperatureunder38C)withlightheadacheandthesymptomrelievedwithouttreatmentwithin24hours.MSCstransplantationiseffectiveandsafeinshort-termforpatientswithAlzheimer'sdisease,andmoreeffectivewhencombinedwithG-CSFtreatment,butlong-termcurativeeffectsneedmoreobservation.LiY乙WangQD,ZhuSW,CuiGX,XieKQ,ShiQ.HouHS,ShenBJ.AutologousbonemesenchymalstemcelltransplantationforAlzheimer'sdiseasein4cases.ZhongguoZuzhiGongchengYaiijiuyuLinchuangKangfu2(X)8;12(43):8431-8433[www.zglckf.com/zglckf/ejournal/upfiles/08-43/43k二431(ps).pdf]摘要：阿尔茨海默病患者10例，按治疗方法分为两组：干细胞原位治疗组6例，给予重组人粒细胞集落刺激因子150pg,腹部皮下注射，1次/d・7-10d为1个疗程；同时给予丹参注射液20mL,静脉滴注，I次/d,10d为1个疗程;自体骨髓干细胞治疗组4例，经骨髓穿刺采集H体骨施230-330mL.分离提取骨腿单核细胞后经静脉途径和/或椎管内一次性或分次注射；注射后当日给予干细胞原位治疗。干细胞治疗前.治疗3个月片进行简易智能测量、日常生活活动能力评分及外周血细胞监测，并对患者神经功能变化及不良反应进行评价。干细胞原位治疗组6例患者未发现任何不良反应，夕卜周血白细胞计数控制在(35-40)x10°I：lo治疗厉3个月时简易智能测量评分及日常生活活动能力评分均高于治疗前(P<0.05)。H体F细胞治疗组4例患者中发热2例，体温均在38£以下，且24h厉体温恢复正常：有轻微头痛3例，未行治疗24h后缓解。治疗后3个月时简易智能测量评分及日常生活活动能力评分均显著高于治疗前(PV0.01)。骨简间充质干细胞移•植治疗阿尔茨海默病近期疗效明显•不良反应较少•与干细胞原位治疗联合应用疗效更佳,其远期疗效还需要进一步随访和观察。关键词：干细胞；骨髓细胞；细胞移植；阿尔茨海默病李义召,王庆德,朱士文,崔贵祥,谢克勤，时庆,侯怀水,沈伯钧•自体骨简间充质干细胞移植治疗阿尔茨海默病4例[J].中国组织工程研究与临床康复,2008,12(43):8431-8433[uww.zglckf.coin/zglckf7ejourna1/upfiles/08-43/43k-8431(p.s).pdf]0引言当前用于治疗阿尔茨海默病的药物很多，但至今尚无治疗阿尔茨海默病的特效药。冇研究表明神经干细胞对阿尔茨海默病的治疗具有潜在的应用前景川。本文观察采用自体骨髓神经干细胞治疗阿尔茨海默病的临床效果及不良反应。1...