右归饮对去卵巢血管钙化人鼠NMDAR1蛋白表达的影响*黎波1龚向京1何虹材2况珊3楚瑞阁I#(1江西中医药大学附属医院南昌330006；2江西中医药大学2014级硕士研究生南昌330004；3江西中医药大学2013级硕士研究生南昌330004)摘要：目的：观察右归饮对去卵巢血管钙化中谷氨酸受体变化的影响。方法：将40只远交群雌性大鼠(spraguedawley,SD)随机分为假手术组、模型组、低剂量组、高剂量组和辛伐他汀组，釆用皮下注射华法令加维生索D3制备血管钙化模型，造模后，低剂量组、高剂量组分别给予右归饮浓缩液5.35g/(kg-d)和16.05g/(kg-d)灌胃，辛伐他汀组给予辛伐他汀混悬水溶液5.25mg/(kg-d)灌胃，假手术组和模型组给予等量生理盐水，共12周；实验结朿时采集血浆标本测定雌二醇及血脂四项，采用苏木精•伊红染色法(HE染色)观察动脉钙化的情况，并采用免疫印迹试验检测N-甲基冬氨酸受体(NMDAR,NR)1蛋白的表达。结杲：模型组大鼠雌二醇、甘油三酯含量和NR1蛋白表达降低，PV0.05,低密度脂蛋白升高，PV0.05；高剂量组和低剂量组雌二醇、甘油三酯含量升高，NR1蛋白表达增加，P<0.05；高剂量组、低剂量组和辛伐他汀组低密度脂蛋白的含量降低，PV0.05；辛伐他汀组雌二醇无变化，P>0.05o结论：右归饮可抑制大鼠去卵巢血管钙化的形成，可能与提高雌激素水平和NR1蛋白的表达有关。关键词：血管钙化；去卵巢；右归饮；NMDARI；蛋白表达InfluenceofYouguiyinonExpressionofNMDARIProteinintheVascularCalcificationofOvaricctomizcdRats*LIBo1,GONGXiang-jingUHEHong-cai2,KUANGShan3,CHURui-gel#(ITheAffiliatedHospitalofJiangxiUniversityofTraditionalChineseMedicine,Nanchang330006;22014Master'sGraduateofJiangxiUniversityofTraditionalChineseMedicine,Nanchang330004;32013Master^GraduateofJiangxiUniversityofTraditionalChineseMedicine,Nanchang330004)Abstract:Objective:ToobservetheinflueneeofYouguiyinonthechangesofglutamatereceptorsinthevascularcalcificationofvariectomizedrats.Methods:FortySDratswereallocatedintotheshamoperationgroup,modelgroup,lovv-doscgroup,high-dosegroupandsimvastatingroup.AfterthearterycalcificationmodelswereestablishedbysubcutaneouslyinjectingwarfarinandvitaminD3,thelow-dosegroupandhigh-dosegroupweregavagedwithconcentratedsolutionofYouguiyinby5.35g/(kgp；and16.05g/(k9thesimvastatingroupwasgavagedwithsuspensionsofsimvastatinby5.25mg/，kg•d),andtheshamoperationgroupandmodelgroupweregivenequalamountofphysiologicalsaline,allfor12weeks・Attheendoftheexperiment,collectedplasmasamplesforthedeterminationofestradiolandscrumlipidsinfour,observedthearterialcalcificationbyhematoxylinandcosinstainingmethod(HEstaining),andusedtheimmuneblottingtodetecttheexpressionofNmethylDasparticacidreceptor(NMDAR,NR)1protein.Results:Theestradiol,triglyceridecontentandNR1proteinexpressionofthemodelgroupweredecreased,Pv0・05,andthelowdensitylipoproteinwaselevated,P<0.05.Theestradiol,triglyceridecontentandNRIproteinexpressionofthehighdosegroupandlowdosegroupweredecreased,P<0.05.Andinthehighdosegroup,lowdosegroupandsimvastatingroup,thelowdensitylipoproteincontentweredecreased,P<0.05.Therewasnochangeintheestradiolofthesimvastatingroup,P>0.05.Conclusion:Youguiyincaninhibittheformationofvascularcalcificationintheovariectomizedrats;itmayberelatedtotheincreasesofthelevelsofestrogencindtheexpressionofNR1protein・Keywords:Vascularcalcification;Ovariectomized;Youguiyin;NMDARI;Proteinexpression中图分类号：R363文献标识码：B血管钙化是指钙磷在血管壁的异常沉积，常发生在血管内膜和中膜层，是动脉粥样硬化的独立危险因素，英发生机理与血管平滑肌、内皮细胞、骨髓间充质干细胞以及造血干细胞相互作用，激活内皮损伤修复过程中相关骨形成相关信号有关，导致血管...