一测多评法同步测定人参和三七药材中多种人参皂苷的含量朱晶晶1,王智民1*,匡艳辉1,张启伟1,高其品2,马妮3(1.中国中医科学院中药研究所,北京100700;2.长春中医药大学,吉林长春130117;3.云南文山州三七研究所,云南文山663000摘要:通过建立人参皂苷Rb1与其他8种皂苷间的紫外相对校正因子(RCF,实现只用一个对照品测定人参和三七药材中多个人参皂苷类成分的含量,以解决人参等药材质量控制中,对照品供应不足问题。结果表明,在一定的线性范围内,人参皂苷Rb1与Rg1、Re、Rf、Rh1、Rc、Rb2、Rb3、Rd间的RCF值分别为1.400,1.215,1.517,1.801,0.944,1.012,1.143,1.135,且在不同实验条件下重现性良好(RSD=0.30%~3.9%。本方法只需测定人参和三七药材中Rb1的含量,其余人参皂苷含量由其RCF值计算得到,实现一测多评;并与常规外标法比较,两种药材中一测多评法与外标法所得结果均无显著性差异;所建立的校正因子可同时用于人参及三七药材及其相关产品的定量分析及质量评价。关键词:一测多评;相对校正因子;HPLC;人参皂苷;人参;三七:R917文献标识码:A:0513-4870(200812-1211-06收稿日期:2008-07-15.基金项目:2007年中医药行业科技专项资助项目(200707009;“”十一五科技支撑计划资助项目(2006BAI08B03-09;中国中医科学院基本科研业务费自主选题资助项目(2006.*通讯作者Tel/Fax:86-10-84014128,E-mail:zhmw123@263AquantitativemethodusingonemarkerforsimultaneousassayofginsenosidesinPanaxginsengandP.notoginsengZHU激ng-jing1,WANGZhi-min1*,KUANGYan-hui1,ZHANGQi-wei1,GAOQi-pin2,MANi3(1.InstituteofChineseMateriaMedica,ChinaAcademyofChineseMedicalSciences,Beijing100700,China;2.ChangchunUniversityofTraditionalChineseMedicine,Changchun130117,China;3.WenshanPrefecturalSanqiScienceandTechnologyResearchInstitute,Wenshan663000,ChinaAbstract:Currentqualitycontrolpatternsarelimitedtoindustrialapplication,formostofthenaturalchemicalreferencesubstancesareexpensiveandunavailable.Herein,amethod,quantitativeanalysisofmulti-componentswithsinglemarker(QAMS,wasestablishedandvalidatedtosimultaneouslydeterminenineginsenosides(ginsenosideRg1,Re,Rf,Rh1,Rb1,Rc,Rb2,Rb3,RdinP.ginsengandfourginsenosides(ginsenosideRg1,Rh1,Rb1,RdinP.notoginseng.UsingginsenosideRb1asthecontrast,therelativecorrectionfactors(RCFoftheothereightginsenosidesweredeterminedbyHPLC-DAD.Withinthelinearranges,thevaluesofRCFofginsenosideRb1toginsenosideRg1,Re,Rf,Rh1,Rc,Rb2,Rb3andRdwere1.400,1.215,1.517,1.801,0.944,1.012,1.143,and1.135,respectively.TheRCFhadagoodreproducibilityinvariousinstruments,chromatographiccolumns(RSD=0.30%-3.9%.AccordingtotheirRCF,wesimultaneouslydeterminednineginsenosidesinP.ginsengonlyusingonemarker.Inaddition,theRCFofginsenosideswereusedtosimultaneouslyquantitativeanalysisoffourginsenosidesinP.notoginseng.TheresultsofQAMSmethodwerevalidatedbycomparingwiththatofexternalstandardmethod,andnoobvioussignificantdifferencewasfound.Keywords:quantitativeanalysisofmulti-componentsbysinglemarker;relativecorrectionfactor;HPLC;ginsenosides;Panaxginseng;Panaxnotoginseng·1211·药学学报ActaPharmaceuticaSinica2008,43(12:1211-1216人参作为名贵中药材广泛应用于临床[1],其主要有效成分为人参皂苷(图1,目前从人参中分离鉴定的人参皂苷已有40余种,可以分为原人参二醇型如Rb1和Rb2,表现为中枢抑制、抗低温、降低细胞内钙、抗氧化等作用;原人参三醇型如Rg1,表现为中枢兴奋,易化学习记忆,促进蛋白、DNA和RNA合成等作用;Rh2则对瘤细胞增殖有抑制[2]。现有人参药材标准以控制Rg1、Re、Rb1等少数几个指标为主。含人参的中成药约有665种,而其标准大多只控制其中的某个成分,难以保证和控制其质量。因此,要提升我国人参的品牌和质控水平,急需建立多成分质量控制标准。已知Rg1、Re、Rb1、Rd、Rf、Rc、Rh1、Rb2...