血液免疫学指标在过敏性紫瘢大鼠和兔模型及患儿体内的比较李彦红I,黄澜I,朱华I,徐艳峰I,隋小龙I,徐玉环I,韩云林I,冯晓纯2,秦川I(1.中国更学科学院，北京协和医学院，医学实验动物研究所，卫生部人类疾病比较庚学重点实验室，国家中医药管理局人类疾病动物模型三级实验室，北京100021；2.长春中医药大学附属医院长春130021)【摘要】目的通过检测过敏性紫瘢大鼠及兔模型的血液学指标的改变，并与人类患者血液学指标改变进行比较，为进一步探索该病的发病机制、明确诊断及治疗提供帮助。方法首先构建模型；进行过敏性紫瘢大鼠及兔的症状观察和病理分析；血常规检测；检测大鼠、兔及患儿血清中细胞因子IL・2、IL・4、TNF・ct,白细胞表面标记分子CD3、CD4、CD8等，免疫球蛋白IgA,IgE,IgG及补体C3,C4的含量。结果模型大鼠和兔皮肤均有不同程度的出血斑点，皮肤，胃肠道，关节及肾脏也表现为不同程度的炎症反应；模型大鼠、兔及患者急性期血常规均有不同程度的白细胞(WBC)升高,淋巴细flfi(LYM)及百分比(LYM%)下降，中性粒细胞(UEN)及百分比(UEN%)升高，血小板(TLP)正常；血清CD4+T细胞减少，CD4/CD8比值下降，IL・4及TNF・(x水平刃•高；IgA升高，C3、C4减少；差别有统计学意义。但这些指标改变的程度不同，血清IL・2、IgG、IgE水平改变不完全一致。结论过敏性紫瘢大鼠及兔模型大部分血液学指标改变与人类相似，可为深入研究该病的发病机制、明确诊断及治疗提供帮助。【关键词】过敏性紫瘢；大鼠模型；兔模型；血液；免疫学ComparisonofBloodImmunologicalIndexesinHenoch-SchonleinPurpuraRatandRabbitModelsandPediatricPatientsLIYan-hong1,HUANGLan1,ZHUHua1,XUYan-feng',SUIXiao-long1,XUYu-huan1,HANYun-lin1,FENGXiao-chun2,QINChuan1(l.KeyLaboratoryofHumanDiseasesComparativeMedicine,MinistryofHealth,InstituteofMedicalLaboratoryAnimalScience,ChineseAcademyofMedicalSciences;KeyLaboratoryofHumanDiseasesAnimalModels,StateAdministrationofTraditionalChineseMedicine,PekingUnionMedicineCollege,Beijing100021,China;2.AffiliatedHospitalofChangchunUniversityofTraditionalChineseMedicine,Changchun130021)【Abstract]ObjectiveThepurposeofthisstudywastooff^rthehelpforexploringthepathogenesis,diagnosisandtherapyofanaphylactoidpurpurathroughdetectionofbloodimmunologicalindexesinratandrabbitmodels,andcomparationwithchangesofbloodindexesinpediatricpatients.MethodsEstablishmentofratandrabbitmodels;Foranaphylactoidpurpura,thentakesymptomobservationandhistopathologicalanalysis;Bloodroutinetests;DetectionofserumcytokinesIL-2,IL-4,TNF-a;whitecellssurfacemarkersCD3,CD4,CDS;immunoglobulinsIgA,IgGIgE,andcomplementsC3andC4contentsinrat,rabbitandpediatricpatients.ResultsRatsandrabbitsmodelsmanifesteddifferentdegreesofskinPetechiae,andgastrointestinal,jointandkidneyalsoshowedvariousdegreesofinflammatoryreaction;Comparedwiththecontrolg---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---roup,ratsandrabbitsofmodelgroupandpatientspresentedincreasednumberofwhitebloodcells(WBC),neutrophils(NEU)andpercentage(NEU%),lymphocytes(LYM)andpercentage(LYM%)decreased,normalcountof(platelet)TLPatacutephase;scrumCD4Tlymphocyteswasdecreased,CD4/CD8ratioweredecreased,andTNF-a,IL-4increased;serumimmunoglobulinIgAlevelelevated,C3,C4decreased,allhadstatisticallysignificantdifferencesbetweenthetwogroups・Buttheseindexeschangedindifferentdegrees,serumIL-2,IgGIgEchangeswerenotthesame.ConclusionsHematologicalchangesofHenoch-Schonleinpurpurainratandrabbitmodelsweresimilartohumans・Theseresultswillprovideavaluablebasisforclarifyingpathogenesis,diagnosisandtreatmentofHSR【Keywords］Henoch-SchonleinPurpura；Ratmodel;Rabbitmodel；Bloo...