卵磷脂/正丙醇/肉豆蔻酸异丙酯/水微乳体系的相结构研究#张奕，蔡建周，魏建业，薛巍**510152025303540（暨南大学生物医学工程系，广东省高校生物材料重点实验室，广州510632）摘要：本文以卵磷脂为表面活性剂，正丙醇为助表面活性剂，肉豆蔻酸异丙酯（IPM）为油相，配制成W/O型微乳。并通过浊点法、电导法、动态光散射法（DLS）以及差示扫描量热法(DSC)研究了随含水量微乳相结构的变化。对于选定配比微乳，实验结果表明：浊点法在含水量超过10.71%时微乳变浑浊；电导率在含水量达到3.85%之前增长缓慢，之后快速增大，直到含水量超过10.71%时电导率下降；DLS显示微乳粒径随含水量先减小后增大，其转折点在5.66%，而含水量超过10.71%后粒径突增三个数量级；DSC曲线在含水量超过3.85%之后出现水的结晶峰，且随含水量的增大而峰位往高温方向平移，同时峰面积增大。当含水量达到11.5%出现两水峰叠加。综上说明含水量从3.85%到5.66%的范围属于W/O向双连续相转变的过程，而含水量10.71%则是体系发生相分离的临界点。基于以上技术的纳米微乳相结构研究直接影响其在药物载体和纳米粒子制备方面的应用性能。关键词：微乳；相结构转变；动态光散射；差示扫描量热法；电导法中图分类号：O648.1InvestigationonPhaseStructureofLecithin/n-Propanol/IsopropylMyristate/WaterMicroemulsionFormulationZHANGYi,CAIJianzhou,WEIJianye,XUEWei(JinanUniversity,DepartmentofBiomedicalEngineering,KeyLaboratoryofBiomaterialsofGuangdongHigherEducationInstitutes,Guangdong,GuangZhou510632)Abstract:Microemulsion(w/o)formulationswerepreparedusinglecithinasasurfactant,isopropylmyristateastheoilphase,n-propanolascosurfactant,andwater.Thewatercontentinfluencethephasebehaviorofthemicroemulsionsystemwasevaluatedviatheconductivitymethod,visualexamination,dynamiclightscattering(DLS)anddifferentialscanningcalorimetry(DSC).Theresultsshowedthat,itbecamecloudywhenmicroemulsionwatercontentwasupto11.5%inthevisualexamination;Theconductivityincreasedslowlywithwatercontentbefore3.85%,andthenfollowedarapidincreaseuntil10.71%atwhichconductivityturnedtodecrease;microemulsiondropletsizesassessedbyDLSdecreasedfirstly,andthenincreasedaswatercontentincreased,theturningpointwasat5.66%.Afterreaching10.71%ofwatercontent,dropletsizesincreaseddramaticallybythreeordersofmagnitude.DSCcurvesrevealedthatmicroemulsiondidnotshowwaterfreezingpeakuntilwatercontentat3.85%.Withtheincreasingofwatercontent,thewaterfreezingpeakshiftedtowardshightemperatureandpeakareaincreasedlinearly.However,thefreezingpeakdividedintotwoatwatercontentof11.5%.Basedonthestudyresults,itcouldbededucedphasestructuretransitionpointfromW/OtoBi-continuouswereinwatercontentfromabout3.85%to5.66%,whilethesystemwasturntophaseseparationat10.71%.Toobtainmoreinformationontheapplicationofmicroemulsionsystemindrugdeliveryaswellasnanoparticlefabrication,itprovedusefultostudythephasestructuretransitionofmicroemulsionwithaseriesofabovemeasuresindetail.Keywords:microemulsion;phasestructuretransition;DLS;DSC;conductometry基金项目：国家自然科学基金资助项目(No.31271019)；广州市天河区科技计划项目（No.096G126）作者简介：张奕，（1983-），男，实验师，主要研究方向：药物载体。通信联系人：薛巍，（1963-），男，博士、研究员，主要研究方向：药物载体、生物材料等。weixue_jnu@hotmail.com-1-4550556065700引言微乳（microemulsion）是由表面活性剂、助表面活性剂、水(或盐水)和油(烃)等多种组分自发形成的一种热力学稳定、各向同性的透明(或大半透明)的油/水分散体系[1]。根据水油比的不同可以分为水包油型、油包水型和双连续相型体系。单相的微乳液其胶束尺寸在100nm以下，制备简易且稳定性良好[2]。一方面广泛应用于各种纳...