中国组织工程研究2013,Vol.17Issue(5):837-842DOI:10.3969/j.issn.2095-4344.2013.05.012肝移植自制移植肝低温保存液对氧自由基的影响Effectsofself-madecryopreservationsolutiononoxygenfreeradicalsincryopreservedratliver全文:PDF(328KB)摘要背景：在肝脏移植过程中，低温保存和缺血可导致肝脏产生氧自由基而损伤肝组织。目的：研究自制器官保存液对低温保存大鼠肝脏氧自由基的影响，并与器官保存液的“金标准”UW液进行对比。方法：16只9周龄SD大鼠随机分为实验组和对照组，每组8只。建立SD大鼠肝脏灌注模型，分别用自制器官保存液组和UW液组灌洗肝脏，取出肝脏置于4℃保存液中，于保存24，48，72h后分别检测肝脏超氧化物歧化酶、一氧化氮合酶、总抗氧化能力和活性丙二醛水平。结果与结论：自制器官保存液对大鼠肝脏低温保存各时点超氧化物歧化酶、一氧化氮合酶、总抗氧化能力活性及丙二醛含量与UW液组比较差异均无显著性意义(P>0.05)，表明自制器官保存液能够减轻缺血再灌注后氧自由基对大鼠肝脏的损伤。自制器官保存液对低温保存大鼠肝脏在减轻氧自由基损伤方面有较好的效果，与UW液相当。关键词：器官移植；肝移植；器官保存液；自制器官保存液；UW液；低温保存；肝脏；移植；氧自由基；省级基金；Abstract：BACKGROUND:Duringlivertransplantation,cryopreservationandischemiacanleadtoproductionofoxygenfreeradicalsbytheliverwhichdamageslivertissue.OBJECTIVE:Tostudytheeffectsofself-madecryopreservationsolutiononoxygenfreeradicalsincryopreservedratliver,andtocomparewith“goldstandard”organpreservationsolution-UWsolution.METHODS:Sixteen9-week-oldSpragueDawleyratswererandomlydividedintotwogroups:experimentalgroupandcontrolgroup,8ratsineachgroup.SpragueDawleyratliverperfusionmodelswereestablished,andtheliversineachgroupwerelavagedwithself-madeorganpreservationsolutionandUWpreservationsolution,thenremovedtheliverandplacedinto4℃preservationsolution.Thesuperoxidedismutase,nitricoxidesynthase,totalantioxidantcapacityactivityandthemalondialdehydecontentweredetectedat24,48and72hoursafterpreservation.RESULTSANDCONCLUSION:Therewerenosignificantdifferencesinsuperoxidedismutase,nitricoxidesynthase,totalantioxidantcapacityactivityandthemalondialdehydecontentinratlivertissuecryopreservedintheself-madeorganpreservationsolutionversusUWsolution(P>0.05).Thesefindingssuggestthatself-madeorganpreservationsolutioncanreducethedamageofoxygenfreeradicaltotheliversofratsafterischemia-reperfusionandtheeffectisequivalenttoUWsolution.Keywords：organtransplantation;livertransplantation;organpreservationsolution;self-madeorganpreservationsolution;UWsolution;cryopreservation;liver;transplantation;oxygenfreeradicals;provincialgrants-supportedpaper;收稿日期:2012-06-12研究亮点:1目前国内器官移植采用的保存液大多是进口UW液，成本高加重广大患者的经济负担。文章观察自制器官保存液，与UW液对比观察同时点低温保存大鼠肝脏超氧化物歧化酶、一氧化氮合酶、总抗氧化能力、活性丙二醛含量，以探讨其对低温保存大鼠肝脏组织氧自由基表达的影响。2实验研制一种新的器官保存液，与UW液对比观察低温保存大鼠肝脏超氧化物歧化酶、一氧化氮合酶、总抗氧化能力、活性丙二醛水平。3结果提示，自制器官保存液成分简单、价格低廉、与UW液相当，其成分具有潜在的可进一步研究的价值。