Abouttheimpactofofcatgutembeddingofclozapinerathypothalamicmonoamineneurotransmitters[Abstract]observedtheofcatgutembeddingofclozapinerathypothalamicmonoamineneurotransmitters[Methods]36SDratswererandomlydividedintothecatgutembeddinggroupclozapinegroup,andblankcontrolgroup.blankcontrolgroup,theothertwogroupsofratswereorallyClozapine5mg·kg-1·d-1increasingto30mg·kg-1·d-1,andthendownto22mg·kg-1·d-1andmaintaineduntiltheendoftheexperiment.clozapine60dcatgutembeddinggroupselecttwosetsofacupuncturepoints:①Baihui,Xinyu(double,QuChi(double,②Ojo,Pishu,(double,Zusanli(double)thealternateembedding,treatment5d,atotalof12times.observedchangesinbodyweightofratsbeforeandaftertreatment,anddetectionofhypothalamicmonoamineneurotransmittersdopamine(DAand5-hydroxytryptamine(5HTcontent.[results]long-termuseofclozapinebodyweightinratscanbeincreasedsignificantly,DAand5HTcontentwas1significantlylower(allP<0.05orP<0.01,catgutembeddingtherapycansignificantlysuppresstheincreaseinbodyweightofratselevatedhypothalamicDAand5HTcontent(P<0.05[Conclusion]Thecatgutembeddingmechanismtomitigateclozapinesideeffectsmaybeassociatedwithincreasedunderthehypothalamicmonoamineneurotransmittercontent.[Keywords]catgutembedding/therapeuticapplicationsofmentaldisorders/acupointtherapymonoamineneurotransmitterdiseasemodelanimalsratClozapine(clozapine)atypicalantipsychotics,anditslowprice,thecurativeeffectisacommonlyuseddrugforthetreatmentofrefractorypsychiatricdiseaseclinicaleasilyleadtoobesity,bloodglucoseandlipidabnormalitiesrangeofsideeffects,butlong-termuseIthasbeenreportedthat[1]Themechanismmayberelatedtotheneurotransmitterandneuroendocrineantipsychoticsbyanti-adrenergicblockingdopamine(DAreceptorandantagonizeserotonin(5HTincreasedbodymassinclinicalexperiments,wefoundthatembeddingtherapycanimprovethequalityofthebodycausedbypatients2takingclozapineincreased,buttheexactmechanismisnotclearyetfoundaccesstoforeignliteraturescholarsreportedthattheexperimentalresearchtoembeddingtherapytoimprovethepossiblemechanismofclozapinesideeffects,theresultsreportedbelow.1Materialsandmethods361.1animalscleanSDrats,male12(246+-12g,female24(250+-9g,ExperimentalAnimalCenterofGuangzhouUniversityofTraditionalChineseMedicinetoprovideCertificateofConformityforSCXK(Guangdong20060001.FeedforordinaryThefullpriceoffeed,providedbytheExperimentalAnimalCenter,GuangzhouUniversityofChineseMedicine.1.2DrugsandreagentsclozapinetabletsXuzhouEnChinaPharmaceuticalGroupCo.,Ltd.production,batchnumber:20050816,treatmentwithcatgutShanghaiPuDongCircleMedicalSuppliesCo.,Ltd.productsspecifications:40,StandardNo.:YY911161999,theindicatorsofbraintissuebytheGuangzhouUniversityofTraditionalChineseMedicine,DepartmentofClinical3Pharmacology,responsibleforinspection.1.3maininstrumentandworkingconditionsDIONEXSummithighperformanceliquidchromatograph(HPLC,theUnitedStatesDIONEXCompany;theChromeleonchromatographyworkstation(AmericanDIONEX),HP1049Aelectrochemicaldetector(Agilent,RC5Clow-temperaturehigh-speedcentrifuge(DuPontPHS2CprecisionpHmeter(ShanghaiLidaInstrumentFactory.Column:DevelosilC30theUG5(150mmx4.6mm,5μm,JapanbyNomuraChemicalCo.,Ltd.),themobilephaseconsistedofpotassiumdihydrogenphosphate(whereinthepotassiumdihydrogenphosphatesolutionwas25mmol/L,containingedetatedihydratesodium0.5mmol/L,pH3.4:acetonitrile=97:3(volumeratio,withphosphoricacidwithavacuum-...