茉莉酸甲酯浸种对水稻幼苗白叶枯病抗性及抗氧化酶活性的影响向妙莲１付永琦１何永明１黄友明１,２曾晓春１,２,∗(１江西农业大学作物生理生态与遗传育种教育部重点实验室,南昌;２宜春学院生命科学与资源环境学院,江西宜春;∗通讯联系人,EＧmail:xchzeng２０１３＠１６３．com)EffectsofSeedSoakingwithMethylJasmonateonResistancetoBacterialLeafBlightandAntioxidantEnzymeActivitiesofRiceSeedlingsXIANGMiaoＧlian１,FUYongＧqi１,HEYongＧming１,HUANGYouＧming１,２,ZENGXiaoＧchun１,２,∗(１KeyLaboratoryofCropPhysiology,EcologyandGeneticBreeding,MinistryofEducation,激angxiAgriculturalUniversity,Nanchang,China;２DepartmentofLifeScienceandEnvironmentalResources,YichunUniversity,Yichun,China,∗Correspondingauthor,EＧmail:xchzeng２０１３＠１６３．com)XIANGMiaolian,FUYongqi,HEYongming,etal．Effectsofseedsoakingwithmethyljasmonateonresistancetobacterialleafblightandantioxidantenzymeactivitiesofriceseedlings．ChinJRiceSci,２０１４,２８(４):４１９Ｇ４２６．Abstract:Thisexperimentwascarriedouttostudytheeffectsofseedsoakingwithexogenousmethyljasmonate(MeＧJA)onresistancetobacterialleafblightandantioxidantenzymeactivitiesofriceseedlingswithWen２２９(susceptibletoricebacterialleafblight)and激azao３１２(resistant)asexperimentalmaterials．TheresultsshowedthatMeJAsoakingsignificantlydecreasedthediseaseindexesofbacterialleafblightinriceseedlings,especiallyfortheresistantcultivar．WithrisingMeJAsolutionconcentration,theactivitiesofsuperoxidedismutase,peroridase,catalaseandascorbateperＧoxidaseinleavesofriceseedlingsincreasedfirstlyandthendecreased,whilethemalondialdehyde(MDA)contentraisedatfirstanddroppedafterwards．Ataconcentrationof０．５mmol/L,MeJAsoakingcauseddamagetoriceseedＧings．TheresponsesofantioxidantenzymeactivitiesandlipidperoxidationcontenttoMeJAsoakingvariedwithvariety．TheaboveresultsindicatedthatMeJAsoakingcouldeffectivelyrelievethebacterialleafblightofriceseedlings．TheinＧcreaseoftheantioxidantenzymeactivitiesandthedecreaseoftheMDAcontentarerelatedtoitsresistantreactionstobacterialleafblight．Keywords:Methyljasmonate;riceseedlings;bacterialleafblight;antioxidantenzyme;lipidperoxidation向妙莲,付永琦,何永明,等．茉莉酸甲酯浸种对水稻幼苗白叶枯病抗性及抗氧化酶活性的影响．中国水稻科学,２０１４,２８(４):４１９Ｇ４２６．摘要:以温２２９(感白叶枯病)和嘉早３１２(抗白叶枯病)为材料,研究外源茉莉酸甲酯(methyljasmonate,MeJA)浸种对水稻幼苗接种白叶枯病菌后抗氧化酶活性及膜脂过氧化的影响.结果表明,MeJA浸种显著降低了水稻幼苗白叶枯病病情指数,对抗病品种的诱导效果优于感病品种;幼苗叶片超氧化物歧化酶(superoxidedismutase,SOD)、过氧化物酶(peroridase,POD)、过氧化氢酶(catalase,CAT)和抗坏血酸过氧化物酶(ascorbateperoxidase,APX)等酶活性随MeJA浓度的升高先增强后减弱,丙二醛(Malondialdehyde,MDA)含量先降低后升高,MeJA浓度为０．５mmol/L时表现为毒害作用.不同抗性品种幼苗抗氧化酶活性和MDA含量对MeJA浸种响应不同,表明MeJA浸种可有效减轻水稻幼苗白叶枯病的发生,其中,抗氧化物酶活性的提高和MDA的下降与水稻对白叶枯病的抗性反应有关.关键词:茉莉酸甲酯;水稻幼苗;白叶枯病;抗氧化酶;膜脂过氧化:文献标识码:A:１００１Ｇ７２１６(２０１４)０３Ｇ０４１９Ｇ０８植物在旱涝、冷热、盐渍、病虫害等逆境胁迫下,植株体内活性氧代谢加强,产生过量O－２、H２O２、OH－等过氧化物自由基伤害细胞膜,损伤蛋白质、膜脂和其他细胞组分,严重影响植株生长发育,甚至收稿日期:２０１３Ｇ０８Ｇ０１;修改稿收到日期:２０１４Ｇ０３Ｇ１７.基金项目:江西省“赣鄱英才５５５工程”领军人才培养计划资助项目;江西省科...