中国组织工程研究2013,Vol.17Issue(6):1094-1100DOI:10.3969/j.issn.2095-4344.2013.06.024干细胞与中医药益气活血类药物血清对嗅鞘细胞增殖及相关因子分泌的影响Serum-containingdrugsresponsibleforsupplementingqiandactivatingbloodcirculationinfluencesproliferationofolfactoryensheathingcellsandsecretionofrelatedcytokines全文:PDF(545KB)下载可见全文摘要背景：益气化瘀是中医药对脊髓损伤的主要治则，但疗效不确切。那么，益气活血类中医药治法能否结合现代科技共同治疗脊髓损伤从而提高疗效呢？目的：观察益气活血类汤剂促进嗅鞘细胞增殖及分泌神经生长因子、脑源性神经营养因子的作用。方法：组织块培养法体外培养新生大鼠嗅鞘细胞；采用MTT法检测补阳还五汤和痿症方含药血清对嗅鞘细胞增殖的影响；采用Western-Blot法测定培养上清中神经生长因子、脑源性神经营养因子水平。结果与结论：MTT结果显示补阳还五汤药物血清及痿证方药物血清均能促进嗅鞘细胞增殖，且两者促增殖作用无差异；Western-Blot结果显示补阳还五汤药物血清及痿证方药物血清均能促进嗅鞘细胞分泌神经生长因子、脑源性神经营养因子，两组间细胞因子分泌水平也无明显差异。关键词：干细胞；干细胞与中医药；补阳还五汤；痿证方；益气活血；含药血清；嗅鞘细胞；增殖；神经生长因子；脑源性神经营养因子；促增殖；组织修复；干细胞图片文章；Abstract：BACKGROUND:SupplementingqiandremovingbloodstasisisthemaintherapeuticprincipleoftraditionalChinesemedicineforthetreatmentofspinalcordinjury,buttheefficacyisnotplausible.But,whethertheapplicationofserum-containingdrugsresponsibleforsupplementingqiandactivatingbloodcirculationundertheguidanceofmoderntechnologycantreatspinalcordinjuryandimprovetheefficacy?OBJECTIVE:Tostudytheeffectofserum-containingdrugsresponsibleforsupplementingqiandactivatingbloodcirculationontheproliferationofolfactoryensheathingcellsandthesecretionofnervegrowthfactorandbrain-derivedneurotrophicfactor.METHODS:Theolfactoryensheathingcellswereinvitroculturedwithtissueblockculturemethod.TheeffectofBuyanghuanwudecoctionandWeizhengdecoctionontheproliferationofolfactoryensheathingcellswasdetectedwithMTTassay.Theexpressionlevelsofnervegrowthfactorandbrain-derivedneurotrophicfactorintheculturesupernatantweredetectedbywesternblotassay.RESULTSANDCONCLUSION:MTTresultsshowedthatbothBuyanghuanwudecoctionandWeizhengdecoctioncouldpromotetheproliferationofolfactoryensheathingcells,andtherewasnosignificantdifferenceinproliferation-promotingeffect.WesternblotassayresultsshowedthatbothBuyanghuanwudecoctionandWeizhengdecoctioncouldpromotetheexpressionofnervegrowthfactorandbrain-derivedneurotrophicfactorinolfactoryensheathingcells,andtherewasnosignificantdifferenceinsecretionlevelbetweentwogroups.Keywords：stemcells;stemcellsandtraditionalChinesemedicine;cultureanddifferentiation;Buyanghuanwudecoction;Weizhengdecoction;supplementingqiandactivatingbloodcirculation;drug-containingserum;olfactoryensheathingcells;proliferation;nervegrowthfactor;brain-derivedneurotrophicfactor;proliferationpromotion;tissuerepair;photographs-containingpaperofstemcells;收稿日期:2012-05-18研究亮点:实验证实益气活血类中药方剂的代表方补阳还五汤及痿证方均能够促进嗅鞘细胞分泌神经生长因子、脑源性神经营养因子，说明益气活血类中药方剂可能通过增强细胞分泌神经营养因子水平来达到促进嗅鞘细胞的增殖。