AbouttheventraltegmentalareamicroinjectionofMKPapersnetwork:[Keywords]morphinedependence[Abstract]AIM:TostudywhethertheblockadeofglutamateNMDAreceptorsintheventraltegmentalarea(VTA)modulatesthemorphinewithdrawalinrats.METHODS:Theeffectsof(+)MK801,microinjectedunilaterallyintotheVTA30minbeforenaloxone(2mg/kg,ip)administration,onthemorphinewithdrawalwereassessed.Morphinedependencedevelopedwithincreasingmorphineinjection(ip)andmorphinewithdrawalwasinducedbyinjection(ip)ofnaloxone(2mg/kg).WetdogshakesandGellertHoltzmanscorewereusedastheindexestoevaluatetheintensityofmorphinewithdrawal.RESULTS:UnilateralmicroinjectionofMK801intotheVTAsignificantlyreducedtheGellertHoltzmanscoreandtheincidenceofwetdogshakesinmorphinewithdrawalrats.CONCLUSION:MK801(2,5,10g/L)unilaterallyadministeredintotheVTAsignificantlyreducestheintensityofmorphinewithdrawalsyndromeindependentrats.Thesedata---本文于网络，仅供参考，勿照抄，如有侵权请联系删除---supportthatglutamatergictransmissionintheVTAanditsinteractionswithdopaminergicneuronsinthemesolimbicsystemplaysomeimportantrolesinopiatewithdrawalsyndrome.OurfindingsalsosuggestapotentialuseofNMDAantagonistsinthetherapeutictreatmentofopiateabuse.[Keywords]MK801;Morphinedeperdence;substancewithdrawalsyndrome;receptors,NmethylDaspartate;Rats;ventraltegmentalarea[Abstract]Objective:Tostudythebrain-theedgeofdopaminecircuitventraltegmentalarea(VTA)ofendogenousglutamatesignaltransductionroleintheformationofthemorphinewithdrawal.Methods:VTAmicro-injectionofdifferentdosesofglutamateNMDAreceptorantagonist(+MK801,toobservetheimpactofthewithdrawalsymptomsofmorphinewithdrawalratswithbehavioralmethods.incrementsofmorphinetoestablishdependencymodelip7d,thelastadministration,1.5hipnaloxonehydrochloride(2mg/kginducedwithdrawalsymptoms,GellertHoltzmanScoreandwetdog-likechatterindicatorsfortheevaluationofthedegreeofwithdrawalsymptomswerestatistically---本文于网络，仅供参考，勿照抄，如有侵权请联系删除---analyzed.Results:(+MK801(2,5,10g/LmicroinjectionintheVTAcanbesignificantlyreducedmorphinequitoffthenumberofoccurrencesofratwetflutterlikeadog,andcansignificantlytheofreducingmorphinewithdrawalratsGellertHoltzmantheScore.conclusion:theVTAendogenousglutamatereceptorsinthebrain-theedgeofdopaminergicsignaltransductioninteractionsinvolvedintheformationofmorphinewithdrawal,suggestingthattheglutamatereceptorantagonistscanbeusedforthetreatmentofmorphinewithdrawal.[Keywords]ventraltegmentalareamorphinedependence,SubstanceWithdrawalSyndromereceptor,N-methyl-D-aspartate,MK801,rats0IntroductionOpioiddependenceisacellularadaptiveresponse,manyofthebrainregionsinvolvedinthebrain-theedgeofdopamineloop-basedone.Studieshaveshownthatmorphinewithdrawalinventraltegmentalarea(VTAdopamineneuronsindiameter,thenumberofreduced[1dischargefrequencyandtheamountofdopamine---本文于网络，仅供参考，勿照抄，如有侵权请联系删除---releasedreduced[2,3],visibleVTAdopamineneuronsdown.VTAdopaminergicneuronsonlyacceptitsownreceptorD2regulation,butalsoacceptglutamatergicprojection.excitedVTAglutamatereceptorcanincreasenucleusaccumbensdopaminereleaseandmotorbehaviorinrats[4],repeatedadministrationofmorphinetoincreaseVTAglutamatereleaseanditsreceptorsubunitexpressionseenVTAglutamatereceptorfunctionincreasesbuttheloopglutamatereceptorroleintheformationofmorphinewithdrawal...