安徽农业科学,JournalofAnhuiAgr.iSc.i2010,38(34):19335-19337,19340责任编辑姜丽责任校对傅真治薄荷叶中黄酮类物质的提取及其抗氧化活性研究邱贺媛,章宋洁(韩山师范学院化学系,广东潮州521041)摘要[目的]优化薄荷(MenthahaplocalyxBriq.)叶总黄酮提取工艺,并研究其抗氧化活性,为薄荷叶总黄酮的提取利用提供依据。4[方法]在单因素试验基础上,以黄酮提取率为指标,采用L9(3)正交试验,对超声波辅助提取,薄荷叶中的黄酮类物质的工艺进行优选。并研究薄荷叶水提液和乙醇提取液对OH的清除作用。[结果]薄荷叶中总黄酮的最佳工艺为:以体积分数40%乙醇为溶剂,料液比130(W/V),超声波功率400W,提取温度50!,提取时间40min,此优化条件下,薄荷叶总黄酮得率达6.58%。薄荷叶水提液对OH有较强的清除作用,且随着总黄酮含量的增加逐渐增大,最高消除率达到44.23%;薄荷叶乙醇提液对OH的清除作用随着黄酮含量的增加先缓慢上升后持续快速下降,总体上低于薄荷叶水提液对OH的清除作用。[结论]利用超声波协同乙醇提取薄荷叶中黄酮类物质的方法简单,可操作性强。薄荷叶中黄酮类物质对OH有较强的清除作用,可以开发利用。关键词薄荷叶;总黄酮;超声波辅助提取法;OH;清除作用+中图分类号S367.235文献标识码A文章编号0517-6611(2010)34-19335-03StudyonExtractingandAntioxidantActivityofFlavonoidsfromMenthahaplocalyxBriq.QIUHeyuanetal(DepartmentofChemistry,HanshanNormalUniversity,Chaozhou,Guangdong521041)Abstract[Objective]TheresearchamiedtooptmiizetheextractionprocessofflavonoidsfromMenthahaplocalyxBriq.leavesandstudyitsantioxidantactivitysoastoprovidethebasisforextractionandutilizationofflavonoidsfromMenthahaplocalyxBriq.leaves.[Method]Takingex4tractionratioofthetotalflavonoidsasindex,L9(3)orthogonaltestwasusedtooptmiizetheextractionprocessofflavonoidsfromMenthahaplocalyxBriq.leavesbyultrasoundassistedextraction.AndtheclearingrateofwaterandethonalextractofMenthahaplocalyxBriq.leavestoOHwasstudied.[Result]TheresultsshowedthattheoptmialprocessofextractingflavonoidsfromMenthahaplocalyxBriq.leaveswasasfollow:ethanolconcentration40%,solidliquidratioof130,ultrasonicpower400W,extractiontemperature50!,andextractiontmie40min.Undertheoptmialconditions,theyieldofflavonoidsfromMenthahaplocalyxBriq.leaveswas6.58%.ThewaterextractofMenthahaplocalyxBriq.leaveshadstrongclearingeffecttoOH,andgraduallyincreasedwiththeraisingofflavonoidsconten;tThebiggestclearingratewas44.23%.TheclearingeffectofethonalextractofMenthahaplocalyxBriq.leavestoOHincreasedslowlyfirs,tthendecreasedquicklyandcontinuouslywiththeraisingofflavonoidsconten,tbutlessthantheeffectofitswaterextractinwhole.[Conclusion]ThemethodofextractingflavonoidsfromleavesofMenthahaplocalyxBriq.byusingethanolandultrasonicwassmiple,andhadstrongmaneuverability.TheflavonoidsfromleavesofMenthahaplocalyxBriq.hadstrongclearingeffecttoOH,andcouldbedevelopedandused.KeywordsMenthahaplocalyxBriq.leaves;Flavonoids;Ultrasoundassistedextraction;OH;Clearingeffect薄荷(MenthahaplocalyxBriq.)又称夜息香、鱼香菜、狗肉香、水益母和鱼香草等,为唇形科(Labiatae)薄荷属(Mentha)1年或多年生宿根性草本植物,是一种生长在低地、路边、河滩、湖边以及园地等处可以药食兼用的野菜,且是一种具有特种经济价值的芳香作物。薄荷全草均可入药,味辛、凉,归肺、肝经,有发散风热、清利咽喉、透疹解毒、疏肝解郁和止痒等功效,可用于治疗感冒发热、头痛、咽喉肿痛、无汗、风火赤眼、风疹、皮肤发痒、疝痛、下痢及瘰疬等症,外用有轻微的止痛作用,可用于神经痛等。薄荷嫩叶除了含有丰富的蛋白质、脂肪和碳水化合物之外,还含有钙、磷、铁、多种维生素和人体必需的微量元素,而可溶性有害重金属(Pb、Cr和Cd)等含量很低。近年来,国内外学者对唇形科很多种植物的...