一串红品种‘神州红’和‘帝王’对高温胁迫的响应刘辉1,2,张国平1,沈国正2,傅巧娟2,阮松林3(1杭州市农业科学研究院园艺研究所,浙江杭州310024;2浙江大学农业与生物技术学院作物科学研究所浙江杭州310029;3杭州市农业科学研究院生物技术研究所,浙江杭州310024)摘要:以自育的一串红新品种‘神州红’与进口品种‘帝王’为材料,在田间模拟自然高温及人工气候箱高温胁迫(40℃)条件下,研究高温胁迫对一串红生育期和田间生长指标以及一串红叶片叶绿素含量(SPAD值)、Fv/Fm(光系统II最大光化学效率)、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)和脯氨酸含量的影响,并比较2个品种耐热性差异。结果表明:田间模拟自然高温条件下,神州红比帝王耐热性强,具体表现在神州红花期较长,冠幅和花序长度均比帝王受高温影响程度小;人工气候箱高温胁迫条件下,2个一串红品种叶绿素含量均降低,但两个品种未显示出耐热性差异;高温胁迫明显降低2个品种Fv/Fm值和SOD酶活性,显著增加CAT和POD活性以及MDA和脯氨酸含量,且2品种间以上性状受高温影响的程度有明显差异;与“帝王”相比,“神州红”光保护机制和活性氧清除系统较强。关键词:一串红;耐热性;形态指标;生理指标中图分类号:S681.4文献标识码:ALIUHui1,2,ZHANGGuo-ping1,SHENGuo-zheng2,FUQiao-juan2,RUANSong-lin3(1.InstituteofCropScience,CollegeofAgriculture&Biotechnology,ZhejiangUniversity,Hangzhou310029,China;2.DepartmentofHorticulture,HangzhouAcademyofAgriculturalSciences,Hangzhou310024,China;3.DepartmentofBiotechnology,HangzhouAcademyofAgriculturalSciences,Hangzhou310024,China)ResponsesofSalviaSplendensVarieties‘Shenzhouhong’and‘Emperor’tothermo-stressAbstract:Inordertostudythethermo-toleranceoftwoSalviasplendensKer-Gawlcultivars,i.e.Shenzhouhong,anationally-bredvariety,andEmperor,animportedvariety,somemorphologicalandphysiologicaltraitsweremeasuredunderhightemperature1基金项目:杭州市科技局科研院所专项(20080732N05)?第一作者简介:刘辉(1979-),男,山东泰安人,在职博士,农艺师,现从事观赏植物栽培技术与分子育种研究.Tel:0571-87313244;E-mail:liuhui518lh@163.com.stress,includingrelativecontentofchlorophyll(SPADvalue),Fv/Fm(maximumquantumyieldofPhotosystemⅡphotochemistry),CAT(catalase),POD(peroxidase)andSOD(superoxidedismutase)activities,MDA(malondialdehyde)andprolinecontents.Hightemperaturestresseswereconductedinthefieldwithnaturalhightemperatureandintheintelligentclimateincubatorwith40℃.TheresultsshowedthatShenzhouhonghadlongerflorescence,anditsrowndiameter,inflorescencedurationlengthwerelessaffectedbythermo-stressthanEmperorundernaturallyhightemperature.Ingrowthincubatorwith40℃,therewasnosignificantdifferenceinSPADvaluesbetweentwovarieties.Whilethermo-stressresultedinsignificantreductionofFv/FmandSODactivity,andsignificantincreaseofCATandPODactivities,MDAandprolinecontents.Moreoverthechangedextentofthesephysiologicaltraitsunderhightemperaturedifferedgreatlybetweentwovarieties.TheresultssuggestedthatShenzhouhongshowedmuchgreaterabilitythanEmperorindevelopinglightprotectiveandactiveoxygenscavengingsysteminresponsetothermo-stress.Keywords:SalviasplendensKer-Gawl;thermo-tolerance;morphologicaltrait;physiologicaltrait一串红(SalviasplendensKer-Gawl)属于唇形科(Labiatae),鼠尾草属(SalviaL)多年生草本植物,原产南美洲热带地区,现在中国各地普遍栽培,常作一年生栽培。一串红具有生长周期短、植株矮壮、适应性强、花色艳丽等特点,因此广泛用来布置城市广场、花坛、花境、道路、庭院等,且其花萼、花冠的红艳色泽为其他花草所不及,宜与浅色花卉配合布置,是中国节日里装点环境、烘托节日...