AbouttheimpactofultravioletradiationonthestorageofthebloodlymphocytefunctionAuthor:WangbaoYan,ZhangJiangengReginawill,ZhuHongmei,WangBaoping,WeiLu[Keywords]lymphocytes[Abstract]AIM:Toexploretheeffectsofultravioletirradiationonthefunctionoflymphocytesinbankedblood.METHODS:Changesoflymphocytesubgroups,lymphocyteactivity,lymphocytetransformation,interleukin2anditsreceptorweretestedbeforeandafterultravioletirradiation,andafterstoragefor72hseparately.RESULTS:Ultravioletirradiationinhibitedthelymphocyteactivity.Ascomparedwiththatofthecontrolgroup,thecontentofinterleukin2decreasedsignificantlywithprolongedstoragetime(P<0.05.Ultravioletirradiationmarkedlyinhibitedthelymphocytestransformation(P<0.01.CD3+,CD4+andCD8+lymphocytesubgroupsinbankedblooddecreasedascomparedwiththoseofprestorageinthetwogroups,buttherewasnosignificantdifferencebetweenthetwogroups.CONCLUSION:Ultravioletirradiationcaninhibitlymphocytesactivityand1lymphocytestransformationandreducetheaccumulationofcytokineinbankedblood.[Keywords]ultravioletrays;bloodpreservation;lymphocytes[Abstract]Objective:Tostudytheimpactofultravioletradiationofstoredbloodlymphocytefunction.Methods:bythebeforeandafterUVirradiationandstorageofbloodsamplesdeterminedafter72hlymphocytesubsetsandactivityofcytokines.Results:UVirradiatedstoredbloodinhibittheactivityoflymphocytesafterirradiationwiththeextensionofstoragetime,thebloodIL2levelsweresignificantlyreduced(P<0.05,afterUVirradiationlymphocytetransformationratesignificantlylowerthanthatinthecontrolgroup(P<0.01,controlgroupstandfor72hwiththebloodofirradiatedgroupofCD3+,CD4+,CD8+cellsubsetsweresignificantlydecreased,butnosignificantdifference.conclusion:ultravioletradiationstoredbloodinhibitlymphocyteactivitywassignificantlyinhibitedproliferationoflymphocytes,canreducethegenerationandaccumulationofcytokines.2[Keywords]ultravioletrays,bloodpreservation,lymphocytes0IntroductionInputcontaininglymphocyteimmuneactivityofbloodcomponentsleadtotransfusion-associatedgraft-versus-hostdisease(TAGVHD,platelettransfusionisineffectiveandnon-hemolyticfebriletransfusionreactions(NHFTRmainreason[1-3].Howtoimprovethequalityofbloodtransfusion,reduceadversetransfusionreactions,andtoensurethesafetyofbloodtransfusionistodayoneofthehotresearchtopics.60Coand137Csr-raysourceirradiatedbloodisamoreeffectivemethod,buttheexpensivedomesticunabletopopularity.hasbeenreported[1-4]UVirradiationofbloodwiththemodulationofimmunefunction,increasetherecipientimmunetolerancetoultravioletradiationinhibitlymphocyteproliferationabilityandsensitivitytoantigenstimulation,thusavoidingthesideeffectsofleukocytecontaminationwestoredbloodtoultravioletradiationandobservethechangesinbloodlymphocytesubsetsandtheircytokines,tofurtherexploretheultravioletirradiationofbloodinthepreventionalloimmunereduceTAGVHDsafetyfeasibility.31Materialsandmethods1.1MaterialsDLX3254computerfull-waveultravioletbloodradiationtherapyinstrument(ChangchuntheYaxingOptoelectronicTechnologyCompany,aone-timethroughtheUVbloodbags(ChangchuntheYaxingOptoelectronicTechnologyCompany,transmittance>80%,typeDG3022Amicroplatereader,Olympusbiologicalmicroscope,lymphocytesthegroupdetectionkit(Fujianthreetomorrowthroughlifetechnologycompanies),humanIL2andsIL2Rquantitativedetectionkit(BelgiumtheBiosourcecompany),PHA(onHayChinaClinicMedicalTechnology,Inc.),RPMI1640powder(U.S.TifeTech...