服用营养素前后精子质量与丙二醛含量的变化[摘要]目的：观察男性不育患者服用营养素前后精子质量，血清及精浆中丙二醛（MDA）含量变化。方法：选择男性不育患者176例，按照WHO精液分析方法进行精液常规检验。采用硫代巴比妥酸比色法测定血清和精浆中MDA含量。本实验采用服用营养素前后自身对照方法进行研究。结果：服用营养素前后患者精子密度均值分别为（31.96±11.09）×106/ml和（49.45±13.07）×106/ml；精子活力均值分别为（27.31±15.91）%和（33.03±23.85）%；精子畸形率均值分别为（39.85±8.47）%和（22.99±9.89）%。精子密度和精子活力明显高于服用前，精子畸形率明显下降（P<0.05）。精子活率高于服用前，但差异无统计学意义（P＞0.05）。服用营养素前后血清中MDA含量均值分别为（0.166±0.081）nmol/L和（0.105±0.016）nmol/L；精浆中MDA均值分别为（0.186±0.087）nmol/L和（0.091±0.013）nmol/L，差异均有统计学意义（均P<0.01）。结论：营养素可提高男性不育患者的精子密度、精子活力，降低精子畸形率，并使血清和精浆中MDA含量下降。本试验结果为男性不育症的诊治和寻找男性优生措施提供了依据。[关键词]营养素；精子；丙二醛；男性不育；优生[]R446.1[文献标识码]Ａ[]1673-7210（2011）12（a）-053-03ChangesofspermqualityandmalondialdehydecontentsbeforeandaftertakingnutrientsGAOYu1,LIRongxiu1,NIUHuimeng2,FANGuorong3,GAOYaping1,LULihua11.HebeiFamilyPlanningInstituteofScienceandTechnology,HebeiProvince,Shi激azhuang500712,China;2.ThePeople'sHospitalofShuangluanDistrict,HebeiProvince,Chengde067000,China;3.XingtangFamilyPlanningTechnicalServiceStation,HebeiProvince,Xingtang050600,China[Abstract]Objective:Toobservethechangesofspermquality,serumandseminalplasmamalondialdehyde(MDA)contentsinpatientswithmaleinfertilitybeforeandaftertakingnutrients.Methods:176patientswithmaleinfertilitywereselectedtoreceivesemenroutinetestaccordingtoWHOsemenanalysismethod.TheserumandseminalplasmaMDAcontentsweremeasuredbyusingthethiobarbituricacidcolorimetry.Thisstudyadoptedself-controlbeforeandaftertakingnutrients.Results:Beforeandaftertakingnutrients,themeanspermconcentrationwere(31.96±11.09)×106/mland(49.45±13.07)×106/mlrespectively,themeanspermmotilitywere(27.31±15.91)%and(33.03±23.85)%respectively,andthemeanspermdeformityratewere(39.85±8.47)%and(22.99±9.89)%respectivelyparedwiththosebeforetakingnutrients,thespermconcentrationandspermmotilityaftertakingnutrientsweresignificantlyhigher,andthespermdeformityratesignificantlydecreased(P<0.05).Thespermmotilityparametersaftertakingnutrientswerehigherthanthosebeforetakingnutrients,buttherewasnosignificantdifference(P＞0.05).Beforeandaftertakingnutrients,themeanserumMDAcontentswere(0.166±0.081)nmol/mland(0.105±0.016)nmol/mlrespectively,andthemeanseminalplasmaMDAcontentswere(0.186±0.087)nmol/mland(0.091±0.013)nmol/mlrespectively,allhadsignificantdifferences(P<0.01).Conclusion:Nutrientscanimprovethespermconcentrationandspermmotility,decreasethespermdeformityrate,andreducetheserumandseminalplasmaMDAcontentsofpatientswithmaleinfertility.Theresultsofthisexperimentprovidebasisfordiagnosingandtreatingpatientswithmaleinfertilityandseekingmaleeugenicmeasures.[Keywords]Nutrients;Sperm;Malondialdehyde;Maleinfertility;Eugenics国内外许多研究报道，由于环境污染和不良饮食习惯所致的一些营养素缺乏等因素会使精液质量下降[1]。一些有害化学物质，如铅、镉、一氧化氮，电磁辐射和锌元素缺乏等因素介导机体的组织细胞脂质过氧化反应，产生过多的氧自由基和脂质降解产物丙二醛（MDA）损伤精子，使精液质量降低。有关研究资料表明[2-3]，使用具有抗氧化作...