侧脑室注射L-AAA致大鼠抑郁样行为改变及电针效应观察符文彬1,姜硕1,刘健华2,孙健1,蒋丽2,樊凌1,袁锋1(1.广州中医药大学第二临床医学院，广州510120；2.广东省中医药科学院针灸电生理重点实验室，广州510120)摘要：目的：观察侧脑室注射L-a-aminoadipicacid(L-AAA)对大鼠抑郁样行为的改变及电针的治疗效应。方法：将45只雌性SD大鼠随机分为空白组、侧脑室注射生理盐水组(侧注NS组)、侧脑室注射L-AAA组(侧注LAAA组)、电针组、利鲁哇灌胃组(药物组),各9只；观察侧脑室注射L-AAA后大鼠行为学的变化及电针、药物对侧脑室注射L-AAA模型大鼠抑郁样行为的改善作用。结果：侧脑室注射L-AAA后大鼠出现体巫下降、新环境抑制饮食时间延长、糖水消耗率下降和运动量减少等类抑郁症状，与空门组及侧注NS组比较，具有显著性差异(P<0.01)；经电针、药物治疗后大鼠的类抑郁症状明显减轻，与侧注射LAAA纽比较，具有显著性差异(PV0.01),基本恢复至正常水平(空白组、侧注NS纽)。结论:侧脑室注射LAAA后可以诱发大鼠的类抑郁症状，而电针可以明显改善此类症状，冃疗效与利鲁哇相似；据此推测电针抗抑郁的机制可能与促进星形胶质细胞修复有关。关键词：电针；抑郁；氨基己二酸；侧脑室注射中图分类号：R245BehaviorinRatsandtheEffectofElectroacupunctureonThatFUWenbim.3,JIANGShuoi,LIUJianhua2,3,SUNJiam,3,JIANGLi2,3,FANLingi?YUANFeng.(1.TheSecondClinicalMedicalCollege,GuangzhouUniversityofTraditionalChineseMedicine,GuangZhou510120;2・TheElectrophysiologylaboratoryofAcupuncture,GuangdongProvincialAcademyofChineseMedicalSciences,GuangZhou510120)Abstract:ObjectiveToobservetheeffectofintracerebroventricularinjectionofL-a-aminoadipicacid(L-AAA)inducingtodepressivebehaviorinratsandtheEfleetofElectroacupuncture(EA)onthat.Methods:45femaleSDratswererandomlydistributedintoblankgroup,intracerebroventricularinjectionofnormalsalinegroup(NSgroup),intracerebroventricularinjectionofL-AAAgroup(LAAAgroup),acupuncturegroup,riluzolcgavagegroup(druggroup),9ratsforeach.ToobservethebehaviorchangesofratsafterintracerebroventricularinfusionofL-AAAandtheeffectofclcctroacupuncturc,drugsonthesedcprcssion-likcbehavioroftheintracerebroventricularinjectionofL・AAAmodelrats.Results:(1)ThereweredepressivesymptomsinratsafterintracerebroventricularinjectionofL-AAA,includingweightloss,thenewenvironmentrestrainfoodtimeprolonged,sucroseconsumptionratedecreasedandexerciseloss,thedifferencewasstatisticallysignificantcomparedwithblankgroupandNSgroup(P<0.01).(2)Theclassofdepressivesymptomsweresignificantlyreducedaftertheelectroacupunctureorriluzolctreatments,thedifferencewasstatisticallysignificantcomparedwithLAAAgroup(P<0.01),andrestoredtonormallevel(blankgroupandNSgroup).Conclusions:IntracerebroventricularinjectionofLAAAcaninducesymptomsofdepressioninrats.Elcctroacupuncturccanimprovesuchdepressionsymptoms,andthetherapeuticeffectissimilartoriluzolc.Wcmayassumethatthemechanismofelcctroacupuncturcantidepressantmightberelatedtopromotingrepairofastrocytes・Keywords:Acupuncture;Depression;L・a-AminoadipicAcid;IntracerebroventricularInjection基金项H：教育部高校博士点专项科研基金（编号：20094425110005）:国家自然基金项H资助（编号:30772828,81173348）:广东省自然科学基金项目资助（编号：07004846）作者简介：符文彬，（1964-）,男，主任医师，研究方向：针灸治疗抑郁相关病症的研究。E-mail:13808888626@139.com0引言抑郁症是一种以持续心境低落为主要临床表现的精神疾病，曾被认为是一种功能性疾病，近年来随着解剖学及影像学的发展，发现抑郁症患者前额叶及海马等部位存在不同程度脑萎缩，提示各种症状的出现可能与大脑某些区域的功能损害相关冋。星形胶质细胞是中枢神经系...