中国组织工程研究第16卷第6期2012–02–05出版ChineseJournalofTissueEngineeringResearchFebruary5,2012Vol.16,No.6ISSN1673-8225CN21-1581/RCODEN:ZLKHAH1007DepartmentofOtolaryngologyHeadandNeckSurgery,AffiliatedZhu激angHospitalofSouthernMedicalUniversity,Guangzhou510282,GuangdongProvince,ChinaWangHai-li,Studyingformaster’sdegree,DepartmentofOtolaryngologyHeadandNeck★Surgery,AffiliatedZhu激angHospitalofSouthernMedicalUniversity,Guangzhou510282,GuangdongProvince,Chinawanghaili.12345@163Correspondenceto:WenZhong,Doctor,Professor,DepartmentofOtolaryngologyHeadandNeckSurgery,AffiliatedZhu激angHospitalofSouthernMedicalUniversity,Guangzhou510282,GuangdongProvince,Chinawenzhong60@163Supportedby:theNaturalScienceFoundationofGuangdongProvince,No.S2011010003947*;ScienceandTechnologyProgramProjectofGuangdongProvince,No.2008B030301344*Received:2011-11-10Accepted:2011-12-22人鼻咽癌CD133+干细胞的生物学特性及意义**★王海丽,文忠,申聪香,钟品能,王军旗BiologicalcharacteristicsandsignificanceofCD133+cancerstemcellsinhumannasopharyngealcarcinomaWangHai-li,WenZhong,ShengCong-xiang,ZhongPin-neng,WangJun-qiAbstractBACKGROUND:Recentstudiesshowsthatcancerstemcellsexistincancercelllines,andmayberesponsibleforrecurrenceandmetastasisoftumor.TherearefewreportsonthebiologicalcharacteristicsofCD133+cancerstemcellsinhumannasopharyngealcarcinoma.OBJECTIVE:ToexplorethebiologicalcharacteristicsandsignificanceofCD133+cancerstemcellsinhumannasopharyngealcarcinoma.METHODS:ExpressionoftheCD133+cellsweredetectedbyflowcytometry,andpurifiedbymagneticcellsortingfromhumannasopharyngealcarcinomacelllineCNE-2.InvitroproliferationandinvivotumorigeniccapacityoftheCD133+stemcellsweredetectedbyserum-freeculture,CCK-8,platecolonyformationtestandtumorigenicityexperimentinnudemice,andwhichstatisticallycomparedwithCD133-andcontrolCNE-2cellstounderstandthebiologicalcharacteristicsoftheCD133+stemcells.RESULTSANDCONCLUSION:CD133+cellsmaintainedasphere-likegrowthstatusinserum-freemediuminvitro,andcouldformtumorspheres.ComparedwiththeCD133-cells,theCD133+cellshadhighercolony-formationability(P<0.01.InvivotumorigenesisabilityoftheCD133+cellsinthenudemicewashigherthanthatoftheCD133-cells(P=0.05.TheresultsconfirmedthatCD133+cellscansuccessfullyseparateandcultureinvitro,andcangeneratetumorspheresandhavehightumorigenesisabilityinthenudemice.WangHL,WenZ,ShengCX,ZhongPN,WangJQ.BiologicalcharacteristicsandsignificanceofCD133+cancerstemcellsinhumannasopharyngealcarcinoma.ZhongguoZuzhiGongchengYan激u.2012;16(6:1007-1010.[wwcrteren.zglckf]摘要背景:有研究表明肿瘤细胞株中存在肿瘤干细胞,是肿瘤复发、转移的根源,但人鼻咽癌细胞株CNE-2中肿瘤干细胞的表达及生物学特性至今少有报道。目的:观察人鼻咽癌CD133+干细胞生物学特性及意义。方法:采用流式细胞仪检测人鼻咽癌细胞株CNE-2中CD133的表达情况。免疫磁珠分选技术获得人鼻咽癌CD133+干细胞,分别采用无血清培养法、CCK-8法、平板克隆形成试验及裸鼠体内成瘤实验检测CD133+干细胞的体外增殖及体内成瘤能力,并将其与CD133-及未分选鼻咽癌细胞进行比较,以了解CD133+干细胞的生物学特性。结果与结论:免疫磁珠富集的CD133+细胞在无血清培养基中呈悬浮生长,并可以形成肿瘤干细胞球。CD133+细胞与CD133-细胞比较具有较高的克隆形成能力(P<0.01;CD133+细胞在裸鼠体内的成瘤率高于CD133-细胞(P<0.05。结果证实,鼻咽癌CD133+干细胞能在体外分离培养,形成干细胞球,增殖能力强,在裸鼠体内具有极强的成瘤能力。关键词:鼻咽癌;肿瘤干细胞;增殖;生物学特性;干细胞培养doi:10.3969/j.issn...