不同剂量蔗糖铁诱导家兔绝经后铁蓄积模型的建立及机制初步探讨邝立鹏叶燕彬江山岳谢成龙罗玉【摘要】目的：评估不同剂量蔗糖铁（ironsucrose，IS）通过耳缘静脉注射诱导家兔绝经后铁蓄积模型建立的可行性及对机制进行初步探讨。方法：普通级雌性家兔50只随机分为5组：假手术组、去勢组以及模型A、B、C组，各10只。去势组和模型组行双侧卵巢切除术，假手术组仅切除卵巢周围部分脂肪组织后即关闭腹腔。术后模型A、B、C组分别通过耳缘静脉注射不同剂量IS（10、15、20mg/kg），假手术组和去势组以同样方式和频次给予适量生理盐水注射。分别于造模前1d和造模后第8周比较各组血清铁蛋白（serumferritin，SF）和17β-雌二醇（estradiol，E2）水平，分析去势组SF与17β-E2的相关性。结果：造模后第8周，模型A、B、C组SF水平均高于假手术组和去势组，且模型C组均高于模型A、B组，比较差异均有统计学意义（P<0.01），去势组17β-E2水平低于假手术组（P0.05）；去势组SF与17β-E2水平无相关性（r=-0.226，P=0.531）。结论：耳缘静脉注射IS可成功诱导家兔绝经后铁蓄积模型的建立，绝经后铁蓄积可能与机体铁排泄途径减少及摄入增多有关。【关键词】蔗糖铁；家兔；绝经后；铁蓄积；动物模型PreliminaryStudyonConstructionofPostmenopausalIonAccumulationRabbitModelsInducedbyVariousDosesofIronSucroseandMechanism/KUANGLipeng，YEYanbin，激ANGShanyue，etal.//MedicalInnovationofChina，2018，15（27）：0-033【Abstract】Objective：Toevaluatethefeasibilityofconstructingthepostmenopausalionaccumulationrabbitmodelsinducedthroughinjectionwithvariousdosesofironsucrose（IS）viatheearvein，aswellastopreliminarilyprobeintothemechanism.Method：50normalgradefemalerabbitswererandomlydividedintofivegroups：shamoperationgroup，castrationgroupandmodelA，BandCgroups，10rabbitsineachgroup.Thecastrationgroupandmodelgroupswereperformedbilateraloophorectomy，whileshamgroupwasconductedremovalofpartoftheadiposetissuearoundtheovary，followedbyabdomenclosureimmediately.ThemodelA，BandCgroupswereinjectedwithvariousdosesofIS（10，15and20mg/kg）viatheearveinpostoperativelyrespectively，andshamoperationgroupandcastrationgroupwereadministeredinjectionwithappropriateamountofsalinewiththesamemethodandfrequency.Thelevelsofserumferritin（SF）and17β-estradiol（E2）ineachgroupwerecomparedat1dbeforemodelingandeighthweeksaftermodeling，thecorrelationbetweenSFand17β-E2incastrationgroupwasanalyzed.Result：Eighthweeksaftermodeling，thelevelsofSFinmodelA，BandCgroupswerehigherthanthoseofshamoperationgroupandcastrationgroup，andthemodelCgroupwashigherthanmodelAandBgroups，thedifferenceswerestatisticallysignificant（P<0.01），thelevelof17β-E2incastrationgroupwaslowerthanthatofshamoperationgroup（P<0.01），buttheSFleveloftwogroupswascompared，thedifferencewasnotstatisticallysignificant（P>0.05）.TherewasnocorrelationbetweenSFand17β-E2levelincastrationgroup（r=-0.226，P=0.531）.Conclusion：InjectionwithISthroughtheearveincaninducetheconstructionofthepostmenopausalionaccumulationrabbitmodels，andthepostmenopausalionaccumulationmayberelatedtothereducedexcretionpathwaysandincreasedintakeofironofthebody.【Keywords】Ironsucrose；Rabbit；Postmenopausal；IonAccumulation；AnimalmodelsFirst-authorsaddress：TheFifthAffiliated（Zhuhai）HospitalofZunyiMedicalCollege，Zhuhai519000，Chinadoi：10.3969/j.issn.1674-4985.2018.27.008铁离子是动物细胞必需的元素之一，参与多种细胞DNA合成、氧转运、细胞呼吸链氧化还原反应等。正常铁代谢构成机体的铁稳态，当铁稳态发生异常时，机体各类系统都会出现相应的代谢紊乱，其中铁过多会催化氧自由基形成，促使细胞膜发生...