中国组织工程研究与临床康复第15卷第3期2011–01–15出版JournalofClinicalRehabilitativeTissueEngineeringResearchJanuary15,2011Vol.15,No.3ISSN1673-8225CN21-1539/RCODEN:ZLKHAH4651BiologicalScienceandEngineeringCollege,SouthChinaUniversityofTechnology,Guangzhou510006,GuangdongProvince,China;2ResearchInstituteofTsinghuaUniversityinShenzhen,Shenzhen518057,GuangdongProvince,ChinaZhangWen-qiang,Master,BiologicalScienceandEngineeringCollege,SouthChina★UniversityofTechnology,Guangzhou510006,GuangdongProvince,China;ResearchInstituteofTsinghuaUniversityinShenzhen,Shenzhen518057,GuangdongProvince,Chinayywenzi23@163.comCorrespondenceto:HuangYue-shan,Doctor,Associateprofessor,BiologicalScienceandEngineeringCollege,SouthChinaUniversityofTechnology,Guangzhou510006,GuangdongProvince,ChinaReceived:2010-08-07Accepted:2010-12-07低相对分子质量透明质酸脂质体的制备及透皮性能★张文强1,2,黄岳山1Preparationoflow-molecular-weighthyaluronanliposomesandtheirpercutaneouspenetrationZhangWen-qiang1,2,HuangYue-shan1AbstractBACKGROUND:Hyaluronicacidisaversatilepolymermaterial,inpracticalapplications,duetoitslargemolecularweight,percutaneousabsorptioneffectisnotsatisfied,theuseofliposomesasacarriercanimproveitstransmissioneffect,withagoodapplicationprospect.OBJECTIVE:Tostudythepercutaneouspenetrationoftheliposomescarryinglow-molecular-weighthyaluronicacid.METHODS:Low-molecular-weighthyaluronanliposomeswerepreparedthroughthinfilms.Orthogonalexperimentwasdesign,andpercutaneouspenetrationwasstudybysnakeslough.RESULTSANDCONCLUSION:Thelow-molecular-weighthyaluronanliposomescouldenwrapmorehyaluronicacidwhenthetemperatureis35,theratioofcholesterolandlecithinis0.151,theratioofhyaluronicacid℃∶andlecithinis0.031,andthe∶pHofhydrationmediumPBSis7.5.Hyaluronicacidcouldpenetratethesnakesloughmoreeasilywhentheywereenwrappedbyliposome,andthepercutaneouspenetrationofhyaluronicacidwasimproved.ZhangWQ,HuangYS.Preparationoflow-molecular-weighthyaluronanliposomesandtheirpercutaneouspenetration.ZhongguoZuzhiGongchengYanjiuyuLinchuangKangfu.2011;15(3:465-467.[http://www.crter.cnhttp://en.zglckf.com]摘要背景:透明质酸是一种用途广泛的高分子材料,在实际应用中,由于其相对分子质量较大,透皮吸收效果不理想,利用脂质体作为其载体可以改善其传输效果,具有较好的应用前景。目的:观察包裹低相对分子质量透明质酸脂质体的透皮性能。方法:采用薄膜法制备低相对分子质量透明质酸脂质体,设计正交实验,采用蛇蜕研究其透皮吸收性能。结果与结论:温度35℃、胆固醇与卵磷脂的比例为0.15∶1,透明质酸与卵磷脂的比例为0.03∶1、水合介质PBS的pH值为7.5,可制备包封率较高的低相对分子质量透明质酸脂质体;脂质体包裹低相对分子质量透明质酸,能有效得提高透明质酸透过蛇蜕的能力,达到了提高透明质酸透皮吸收率的作用。关键词:低相对分子质量;透明质酸;脂质体;透皮吸收;包封率doi:10.3969/j.issn.1673-8225.2011.03.021张文强,黄岳山.低相对分子质量透明质酸脂质体的制备及透皮性能[J].中国组织工程研究与临床康复,2011,15(3:465-467.[http://www.crter.orghttp://cn.zglckf.com]0引言透明质酸,又名玻璃酸,是一种独特的线性大分子酸性黏多糖,由葡萄糖醛酸和N-乙酰氨基葡萄糖的双糖单位反复交替连接而成,广泛分布于动物和人体结缔组织细胞外基质中,在眼玻璃体、脐带、皮肤、软骨和滑液中含量较高。透明质酸为白色、无定形粉末,有吸湿性,不溶于有机溶剂,易溶于水,具有良好的保湿性、润滑性、黏弹性、非免疫原性和生物相容性,是一种用途广泛的高分子材料,在临床治疗、诊断和化妆品基质等方面发挥其独特的作用[1-2]。在实际应用中,由于透明质酸相对分子质量较大,不能透...