纳米液态氟碳脂质微球超声造影剂的制备及体外聚集实验研究陈松1，康娟，王志刚，李攀，伍星，许川山（重庆医科大学附属第二医院超声影像学研究所，重庆400010）摘要：目的制备一种新型超声造影剂－纳米液态氟碳脂质微球造影剂，并研究其体外基本特性及聚集后增强超声显影性能。方法采用高压均质法制备纳米液态氟碳脂质微球超声造影剂，检测一般理化性质，再制备生物素化微球，以空白微球为对照组，分别在加入亲和素前后，观察体外聚集情况及超声显影效果，并用DFY超声图像定量分析统计软件计算、比较超声图像平均灰度值。结果所制备的液态氟碳脂质微球外观呈乳白色的混悬液，镜下微球形态圆整，大小均一，性质稳定，粒径（171.9±91.0）nm；制备的生物素化微球在加入亲和素后聚集成团状，并可增强超声显影，经DFY软件分析，加入亲和素前后超声图像平均灰度值差异有统计学意义。结论成功制备出稳定的纳米液态氟碳脂质超声造影剂，并采用生物素亲和素法证明了该微球具有聚集后增强超声显影性能。关键词：纳米；液态氟碳；超声造影剂中图法分类号：文献标识码：APreparationandinvitroaggregationstudyofanovelultrasoundcontrastagent,anovelperfluorocarbonlipidmicrolipheresnanoparticlesultrasoundcontrastagentCHENSong,KANGJuan，WANGZhi--gang,LIPan,WUXing,XUChuan-shanXUChuan-shan（(InstituteofUltrasoundImaging,ChongqingMedicalUniversityofMedicalSciences,Chongqing400010，,China）)Abstract：ObjectiveTodevelopanovelnanoscaleultrasoniccontrastagent—,perfluorocarbonlipidnanoparticlesmicrolipheresandtoinvestigatetheiritsbasiccharactoeristicssandcapabilitytoenhanceultrasoundimageswhenaggregated.MethodsPerfluorocarbonlipidmicrolipheresnanoparticleswerepreparedbylecithinandliquidperfluorocarbonwithahighpressurehomogen.Thephysico-chemicalpropertiesofthemicrolipherenanoparticlswaseredetected.StepavidinwasaddedtothepreparationproceduretoproduceBbiotinylatedperfluorocarbonlipidnanoparticles.microliphereswerepreparedBlanknanoparticleswithorwithoutbiotinylationservedascontrol.The4kindsofnanoparticleswereusedforultrasoundimagingbeforeandafteraddingstepavidin.Theaggregation,contrasteffectoftheseagentswerestudied,andtheaveragegrayscalevalueofthecapturedultrasoundimageswereanalyzedbytheDFYsoftware.ResultsThesemicroliphernanoparticleswereuniformandstableilized,andwithathemeansizeisof（(171.9±91.0）)nm.Therewasnovisuablevisibleultrasoundsignals1基金项目：“863”科技攻关项目(2006AA02Z4F0);国家自然科学基金重点项目(30430230);重庆市自然科学基金面上项目(2005BA5024)SupportedbytheTacklingProgramoftheNational“863”KeyResearchProjectofChina(2006AA02Z4F0),theKeyProgramoftheNationalNaturalScienceFoundationofChina(30430230)andtheGeneralProgramoftheNaturalScienceFoundationofChongqing(2005BA5024)作者简介：陈松，男，重庆市人，硕士研究生，主治医师，主要从事超声造影方面的研究。电话：（023）63719612，E-mail：cs20012001@163.com通信作者：王志刚，电话：（023）63719612收稿日期：2008-01-17；修回日期：2008-07-28scatteredbackfromwiththenanoparticlesmicrolipheresbeforeaddingstrepavidin,whileobviouslyultrasoundechosignalswereobservedinthosebiotinylatednanoparticlesafteraddingstrepavidin.,withTherewassignificantdifferencebetweentheultrasoundimagesbefore/afterbiotinylationaddingstrepavidin,whichwereanalyzedbyDFYsoftware.ConclusionsAsteadyperfluorocarbonlipidnanoparticlesmicrolipheresweareprepared,andthebiotinylatedmicrospherescanouldaggregatetogetherintheexistenceofstrepavidinandenhancetheultrasoundimage.Theaggregatednanoparticlesmicrolipheresmightbetakenregardedasakindofultrasoundcontrastagent,whichlaysthe...