药用真菌发酵及其产物对AA肉鸡免疫功能及生长的影响摘要:以一药用真菌进行固体发酵,比较不同培养基质、起始含水量、起始pH值对其生长的影响;再以该真菌发酵物(菌质)作为饲料添加剂试验,研究其对AA肉鸡生长性能和免疫功能的影响。结果显示:以大米、小米和复合基质进行固体发酵,该真菌生长较好(生物量为3518%),且添加一定量的中药不影响真菌的生长,起始含水量为50%,起始pH值为415;动物试验表明,含中药的发酵产物能明显增强AA肉鸡对新城疫,禽流感H9、H5的免疫力(P<0105,P<0101),体增重显著高于对照组(P<0105,P<0101)。关键词:药用真菌;免疫力;肉鸡;体增重StudiesonmedicinalfungifermentationandeffectsofitsproductsonthemimunityfunctionandgrowthperformanceofAAbroilerZHANGL-iyang,ZHOUYe-fe,iZHANGDun-lin(LifeScienceDepartmentofNanjingXiaozhuangInstitute,Nanjing210017,China)Abstract:Thesolidfermentationwithpharmaceuticalfungiwasstudied.Theeffectsofdifferentmedium,initialwatercontentandinitialpHongrowthofpharmaceuticalfungiwerecompared.Themedicinalfungalsubstancebysolidfermentationwithsomeconditionswasactedasfeedadditive,thentheeffectofmedicinalfungalonimmunefunctionandgrowthperformanceofAAbroilerwasstudied.Theresultsindicatedthatthegrowthoffungiwithsolidfermentationundertherice,milletandcompositemediumwasbetterwithitsbiomassof3518%.SomeCh-inesemedicineinthefermentationmediumdidnctaffectfungigrowthwhentheinitialwatercontentandinitialpHwere50%and415.Fermen-tationproductoffung,iincludingChinesemedicine,couldenhanceAAbroilercsimmunityagainstNewcastledisease(ND),H9收稿日期:2004-10-24基金项目:江苏省教育厅科技项目(2004nxy01)。---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---作者简介:张李阳(1961-),女,副教授,硕士。因的大肠杆菌繁殖时,带有淀粉酶基因的质粒随着分裂进入到新的细胞体内。故在大肠杆菌大量繁殖时,淀粉酶活力也随着提高。③大肠杆菌分泌淀粉酶的机制可能优于枯草杆菌。本试验中,选择了2种培养液来增殖细菌。LB是一种常用的细菌培养液,似乎对原始枯草杆菌淀粉酶分泌有促进作用。矿物元素培养液对转基因的大肠杆菌分泌淀粉酶能力有促进作用,这可能是淀粉诱导淀粉酶产生的原因。因而在实际生产中,要考虑对不同的菌种选择最适宜的培养液,以便于最大限度地提高淀粉酶的产生。参考文献:[1]张树政.酶制剂工业[M].北京:科技出版社,1984,466-467.[2]LinLL,HsuWH.Lactose-inducedexpressionofBacillussp.TS-23amylasegeneinEscherichiacoliregulatedbyaT7promoter[J].LettersinAppliedMicrobiology,1997,365(1):235-239.[3]AusubelFM,BrentR,KingstonRE,eta.lShortProtocolsinMo-lecularBiology.Thirdedition[M].NewYork:JohnWileyandSons,INC,1995,1-23.[4]ChoHY,KimYW,KimTJ,eta.lMolecularcharacterizationofadimericintacellularmaltogenicamylaseofBacillussubtilisSUH4-2[J].BiochemicalandBiophysicalACTA,2000,2:333-343.[5]PenJ,OoyenAJJ,ElzenPJM,eta.lDirectscreeningforhigh-levelexpressionofintroducedalpha-amylasegeneinplants[J].PlantMolecularBiology,1992,18:1133-1139.[6]GiraudE,GosselinL,MarinB,eta.lPurificationandcharacteriza-tionofanextracellaramylasefromLactobacillusplantarumstrainA6[J].JournalofAppliedBacteriology,1993,75:276-282.[7]李影林.临床医学检验手册[M].长春:吉林科学技术出版社,1987,378-379.---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---[8]BrownTA.Genecloning[M].SecondEdition.NewYork:Chap-manandHal,l1990,13-43and84-102.#9#畜牧与兽医2005年第37卷第6期andH5.Thebodyweightgainsinexperimentgroupswashigherthanthatinthecontrolgroup(P<0105,P<01001).Keywords:pharmaceuticalfung;iimmunity;broiler;bodyweightgain在现代养殖业中,为促进畜禽生长,提高畜产品产量,饲料...