不同起源胶质瘤中microRNA-210表达差异及其和星形胶质细胞肿瘤级别相关性的研究赖年升1张帅1成宜军2徐幸1蔺玉昌11.南京医科大学附属无锡第二人民医院神经外科江苏无锡2140002,南京医科大学附属脑科医院江苏南京210029【摘要】目的探讨microRNA-210（miR-210）在星形胶质细胞肿瘤和少突胶质细胞肿瘤中的表达差异及意义。方法将组织标本分为正常脑组织、少突胶质细胞肿瘤、星形胶质细胞肿瘤三组，星形胶质细胞肿瘤再分为A[毛细胞型星形细胞瘤（WHOⅠ级）]，B[弥漫性星形细胞瘤（WHOⅡ级）]，C[间变性星形细胞瘤（WHOⅢ级）]，D[胶质母细胞瘤（GBM,WHOⅣ级）]。采用实时定量PCR方法检测各组中miR-210的表达水平。统计学分析miR-210的表达和星形胶质细胞肿瘤的相关性。结果miR-210在各级别星形胶质细胞肿瘤中表达水平依次为D,C,(B,A)(P<0．001)，除A、B组间外，其他组间都具有统计学意义(P<0．001)，说明星形胶质细胞肿瘤级别越高，miR-210表达水平越高；然而在少突胶质细胞肿瘤中呈低表达(P<0．05),且间变性少突胶质细胞瘤中表达量低于少突胶质细胞瘤中的表达量。结论miR-210在不同起源、不同级别胶质瘤中的表达水平不同，可以作为不同起源胶质瘤病理检查的辅助鉴别手段，也可作为星形胶质细胞肿瘤恶性进展的生物学标志物。miR-210的表达水平与星形胶质细胞肿瘤和少突胶质细胞肿瘤病理分级具有相关性，miR-210的表达量随着星形细胞肿瘤级别的增高而增加，随着少突胶质细胞肿瘤级别的增高而降低，提示miR-210与胶质瘤的预后密切相关。miR-210表达水平在二种不同起源的胶质瘤中表达具有差异，在少突胶质细胞肿瘤中表达降低，在星形胶质细胞肿瘤中表达增高。【关键词】胶质瘤；星形胶质细胞肿瘤；少突胶质细胞肿瘤；MicroRNA-210；预后DifferentialexpressionofmicroRNA-210ingliomasofvariablecelloriginandcorrelationofincreasedexpressionlevelstodiseasegradeinastrocytictumorsLAINian-sheng*1,ZHANGShuai1,CHENGYi-jun2,XUXing,LINYu-chang*11.DepartmentofNeurosurgery,WuxiSecondPeople'sHospitalAffiliatedtoNan激ngMedicalUniversity,WuxiCity214002,China2.Nan激ngBrainHospitalAffiliatedtoNan激ngMedicialUniversity，Nan激ng210029，ChinaCorrespondingauthor:LINYu-chang,Email:yuchanglin123@126【Abstract】ObjectiveToinvestigatethesignificanceandexpressionofmicroRNA-210inastrocytictumorsandoligodendroglialtumors.MethodsTissuesamplesweredividedintothreegroupsofthenormalbraintissue，theoligodendroglialtumorandtheastrocytictumor;TheastrocytictumorsweredividedintothreegroupsofA[pilocyticastrocytoma(WHOgradeI)]，B[diffuseastrocytomas(WHOgradeII)],C[anaplasticastrocytomas(WHOgradeIII)],andD[glioblastoma(GBM,WHOgradeIV)].TheexpressionofmiR-210wasexaminedbyreal-timequantitativeRT-PCR.ThecorrelationoftheexpressionofmiR-210andtheastrocytictumorswereanalyzedbytheSpearmancorrelationtest.ResultsTheexpressionlevelofmiR-210wasinanorderofgroupD>groupC>(groupBandgroupA)(P<0．001).TherewasstatisticallysignificantdifferencebetweengroupsexceptgroupAandB(P<0．001).ThegradeofastrocytictumorandtheexpressionofmiR-210hadpositivecorrelation.TheexpressionlevelofmiR-210inastrocytictumoroligodendroglialtumortissueswassignificantlyhigherthannormalbraintissues，---本文于网络，仅供参考，勿照抄，如有侵权请联系删除---通讯作者：蔺玉昌，Email：yuchanglin123@126butwaslowerinoligodendroglialtumor(P＜0.001).ConclusionSinceexpressionofmiR-210showedsignificantdifferencesindifferentoriginanddifferentgrades,itcanbeusedasanauxiliaryidentificationmethodforthehistopathologicexaminationofgliomafromdifferentorigin,orasabiomarkerfortheobservationoftheprogressofastrocytetumoraswell.【Subjectwords】Glioma;Astrocytictumor;Oligodendroglialtumor;MicroRNA-210脑...