《文献信息检索》综合作业3要求：1．通过实习，巩固所学的检索策略及资源类型。2．写出所用检索词、检索表达式及命中篇数并复制粘贴2篇在文挡中。3．课堂作业评点和分析时，希望积极参与教学互动。一、医学数据库检索1、Pubmed库（1）检索福建中医药大学吴锦忠教授发文情况，分别以题录（Summary）和文摘（Abstrats）格式下载保存。答案：检索式：Wu,激nzhong[FullAuthorName]检索结果：3文摘方式：1.JNatMed.2013Jan;67(1):17-26.doi:10.1007/s11418-012-0639-x.Epub2012Mar1.InvestigationoftheextractsfromBidenspilosaLinn.var.radiataSch.Bip.forantioxidantactivitiesandcytotoxicityagainsthumantumorcells.WuJ,WanZ,YiJ,WuY,PengW,WuJ.Fu激anUniversityofTraditionalChineseMedicine,Fuzhou,People'sRepublicofChina.TheaimofthisstudywastoevaluateantioxidantactivitiesandcytotoxicityagainsthumantumorcelllinesofextractsfromBidenspilosaLinn.var.radiataSch.Bip.(BP).Totalphenolicandflavonoidcontentsoftheextractsweredeterminedbyultravioletspectrophotometry.Furthermore,theantioxidantpropertiesofdifferentpolarityfractionsextractedfromBPwereevaluatedusingDPPHandABTSradicalscavengingtestandFRAPassay.Theethylacetatefraction(EE-BP)showedthehighestantioxidantactivitycomparedtootherfractions.Inaddition,theanti-proliferativeactivitiesoftheextractsonfourhumantumorcells,namelyMCF-7,HepG2,MGC803andRKO,wereinvestigatedbyMTTmethod.TheEE-BPdisplayedthemostremarkableanti-proliferativeeffectagainstthetumorcells,particularlyRKOcellindose-andtime-dependentmanner.Theantioxidantactivitiesandcytotoxicitycorrelatedhighlywiththetotalphenolicandflavonoidcontents,respectively.Furthermore,TheactiveingredientBP-6,namely5,7,4'-trihydroxy-3,3'-dimethyl-flavonol,wasisolatedandpurifiedwiththepurityabove99.00%andcontentof0.15%inEE-BPdetectedbyHPLC,whichcouldsignificantlyinhibittheproliferationofRKOcellswiththeIC(50)valueof6.66μmol/l.InordertocharacterizetheapoptoticRKOcells,flowcytometryandDNAfragmentationassaywereperformed.Apoptoticcellnumbersincreasedinadose-dependentmannerafterthetreatmentwithdifferentconcentrationsofEE-BPandBP-6for12and6h,respectively.DNAladdersinapoptoticRKOcellscouldbeeasilyvisualizedwhenexposedto200μg/mloftheEE-BPfor36h.Takentogether,ourworkindicatedthatBPhadpotentiallytherapeuticvalueagainstcolorectalcancer.PMID:22382861[PubMed-indexedforMEDLINE]2.CurrMicrobiol.2011Apr;62(4):1160-7.doi:10.1007/s00284-010-9838-4.Epub2010Dec15.Differentialproteomicanalysisoftemperature-inducedautolysisinmyceliumofPleurotustuber-regium.HuangB,LinW,CheungPC,WuJ.SchoolofLifeSciences,Fu激anAgricultureandForestryUniversity,Fuzhou350002,China.Autolysisisanimportantphysiologicalprocessfoundinfungalcultivation.However,thereishithertonoreportontheautolysisofPleurotustuber-regium.Wehaveinvestigatedtheenzymessecretedbytemperature-induced(40°Castreatmentversus10°Cascontrol)autolysisofthemyceliumofP.tuber-regiumgrowninsubmergedcultivation.Acomparisonbetweentheintracellularproteins(insidethemycelium)andtheextracellularproteins(intheculturemedium)ofthetreatmentandcontrolbyproteomicanalysisinvolving2DPAGEandMALDI-TOF-MSwasmade.Twenty-twoup-regulatedproteinspotsweredetectedandeightproteinswereidentified.Theyincludedproteasomewhichparticipatesintheubiquitin-proteasomepathway;β-1,3-glucanosyltransferaseandtubulinwhichareinvolvedintherenewalandrepairofcellwall;proteaseandendoglucanasewhichpromotethenaturaldegradationofcellwallandcytoplasm;14-3-3proteinwhichtakespartinc...