夏枯草对草酸结石小鼠钙代谢及肾脏组织结构的影响李静胡国全桂敬强贺绍君刘德义陈会良靳二辉（安徽科技学院动物科学学院安徽省凤阳县东华路9号233100）摘要：目的探讨夏枯草对草酸结石小鼠的钙代谢及肾脏组织结构的影响。方法用乙二醇和氯化铵诱导结石，用夏枯草干预，第28d，测定血清中钙和磷含量，测定肝脏、右侧肾脏和脑组织匀浆中的钙含量，左肾用于制作HE染色切片。结果结石组肾钙显著高于对照组（P<0.05），而夏枯草组肾钙显著低于结石组（P<0.05），与对照组差异不显著。各组的血清、肝、脑组织匀浆中钙含量差异均不显著。结石组的肾小管腔中分布大量散在的透明草酸钙晶体，夏枯草组肾组织未见草酸钙晶体沉积，且肾小管扩张情况明显缓解。结论夏枯草可减少草酸结石小鼠的肾钙含量，减缓肾小管的扩张，保护肾小管上皮细胞组织结构，可做为防治草酸结石的辅助药物。关键词：夏枯草；草酸结石；肾；小鼠中图分类号：S853.7文献标志码：A文章编号：EffectofPrunellavulgarisL.onCalciumMetabolismandRenalStructureofCalciumOxalateStonesinMiceLIJing*,HuGuo-quan,GuiJing-qiang,HeShao-jun,LiuDe-yi,ChenHui-liangJinEr-hui(CollegeofAnimalScience,AnhuiScienceandTechnologyUniversity,Fengyang,Anhui233100,China)Abstract:ObjectiveToinvestigatetheinterventioneffectofPrunellavulgarisL.oncalciumoxalatestonesinmiceofcalciummetabolismandrenalstructure.MethodThemicewithcalciumoxalatestoneformationwereinducedby1%ethyleneglycoland1%ammoniumchloride,meanwhileusedPrunellavulgarisL.asinterveningfactors.After28days,thecalciumandphosphoruscontentwasmeasuredinserum.Thecontentofcalciuminliver，rightkidneyandbrainorganizationhomogenateweredetected．HistologicalsectionofleftkidneyweremadeHEstaining.Thecalciumcontentandphosphatecontentinkidneytissuesawasdetermined.HistologicalsectionofkidneyweremadeHEstaining.ResultsThatcalciumlevelsinrenaltissueofOxalateCalciumStonegroupwassignificantlyhigherthannormalcontrolgroup（P<0.05）,calciumlevelsinrenaltissueofPrunellavulgarisL.OxalateCalciumStonegroupwassignificantlylowerthanOxalateCalciumStonegroup（P<0.05）,andthannormalcontrolgroup收稿日期：基金项目：安徽科技学院自然科学研究项目（ZRC2013366）；安徽科技学院重点学科基金项目(AKXK20101-2)作者简介：李静（1979－），女，黑龙江省齐齐哈尔人，讲师，博士。从事中草药防治结石疾病方面的研究，Tel：0550-6734670，E-mail：lijing3719@163.com。李静为通讯作者。wasnosignificantdifferences.Thecontentofcalciuminliverandbrainorganizationhomogenateineachgroupswasnosignificantdifferences.ThecalciumoxalatestonesinrenaltubularwerevisibleintheOxalateCalciumStonegroup,crystalsdepositionofcalciumoxalatestonesinPrunellavulgarisL.groupweresignificantlyreduced,significantlyreducerenaltubulardilation.ConclusionThePrunellavulgarisL.couldreducethecalciumcontentinrenalorganizationhomogenate,ureanitrogenandcreatininecontentinthebody,andslowtheexpansionofrenaltubular,protectionofrenaltubularepithelialcellstructureinmiceofcalciumoxalatestones,canbeusedassupplementarydruginpreventingoxalatecalcium.Keywords:PrunellavulgarisL.;calciumoxalatestones;kidney;mice夏枯草（PrunellavulgarisL.）属于唇形科夏枯草属植物，命名取其“夏至后即枯”之意，药用部位为干燥果穗，全草也可入药[1]，主要含有三萜及其苷类、甾醇及其苷类、黄酮类、香豆素、苯丙素、有机酸、挥发油及糖类等成分[2]。夏枯草在调节血压和血糖，消炎杀菌，抗病毒感染，抑制肿瘤生长方面广泛应用[3]。Utsunomiya[4]等首次发现在体外模拟试验中夏枯草水提取液能有效抑制草酸钙结晶的形成。夏枯草水提取液和甲醇提取物在体外对草酸钙结晶形成均有抑制作用[5]，推测其原因可能夏枯草促进草酸钙结晶的...