儿丁糖.透明质酸嗨治疗多西紫杉醇外渗性大鼠皮肤损伤朱其聪，李爱民，罗荣城，梁卫江，戴明，陈晓华EfficacyofChitosanandHyaluronidaseonSkinDamageCausedbyDocetaxelExtravasationinRatsZHUQi-Cong,LIAi-Ming,LUORong-Cheng,LIANGWei-激ang,DAIMing,CHENXiao-Hua[ABSTRACT]BACKGROUNDOBJECTIVE：Docetaxelextravasatingintothesurroundingtissuesmayleadtosevereskindamage・NoguidelineforhandlingdocetaxelextravasationhasbeenproposedtillnoThisstudywastoexploretheefficacyofchitosanandhyaluronidaseonskindamagecausedbydocetaxelextravasationinaratmodel.METHODS：Adocetaxelextravasationmodelwasestablishedinbothlowerextremitiesof30Sprague-Dawleyrats.Theratsweredividedinto6groupsandreceivedchitosanembrocation,hyaluronidaseinjection,hyaluronidaseinjectionpluschitosanembrocation,salineembrocation,orsalineinjection,orreceivednotreatmentascontrol.Theoccurreneerateandextentofskindamage,andthehealingtimewereobservedandcompared.RESULTS：Theoccurreneerateofskindamagewassignificantlylowerinhyaluronidasegroupandhyaluronidasepluschitosangroupthaninchitosangroup,salineembrocationgroup,salineinjectiongroup,andcontrolgroup(30%and20%vs.90%,100%,90%and100%,Pv0.05)・Thehealingtimewassignificantlyshorterinhyaluronidasegroupandhyaluronidasepluschitosangroupthaninchitosangroup,salineembrocationgroup,salineinjectiongroup,andcontrolgroup[(12.00±3.00)daysand(9.50±2.12)daysvs.(18.33±2.00)days,(23.70±2.41)days,(18.44±2.01)daysand(25.70±2.26)days,Pv0・01],andwassignificantlyshorterinchitosangroupthaninsalineembrocationgroupandcontrolgroup(Pv0.01)・CONCLUSIONS：Hyaluronidasealoneorhyaluronidasepluschitosancoulddecreasetheoccurreneerateofskindamagecausedbydocetaxelextravasationinrats,andshortenthehealingtime.Chitosanembrocationcanimprovethedamagehealing,butcan'tdecreasetheoccurreneerateofskindamage・KEYWORDS：Chitosan/treatment；Hyaluronidase/treatment；Docetaxel；Drugextravasation；Skindamage；Rat【摘要】背景与目的：多西紫杉醇外渗至周围组织可导致严重皮肤损伤，目前还没有公认的治疗指南。本研究旨在探讨单用或联合局部外涂儿丁糖、注射透明质酸酢对多西紫杉醇大鼠外渗性皮肤损伤的疗效。方法：在30只SD大鼠双后肢建立多西紫杉醇血管外渗模型。随机分为6组，分别给局部外涂几丁糖、局部注射透明质酸酶、局部注射透明质酸酶联合外涂儿丁糖、局部外涂生理盐水、局部注射牛理盐水和不进行任何处理(模型对照组)。观察各组大鼠外渗性皮肤损伤发牛•率、损伤程度和损伤愈合时间。结果：透明质酸腮组和透明质酸I晦联合儿丁糖组的损伤发生率分别为30%和20%,显著低于儿丁糖组、生理盐水外涂组、生理盐水注射组和模型对照组的损伤发生率(90%、100%、90%、100%)(P<0.05)o透明质酸酶组和透明质酸酶联合几丁糖组的损伤愈合时间为(12.00±3.00)天和(9.50±2.12)天，显著短于其它4组(P<0.01)o几丁糖组的损伤愈合时间为(18.33±2.00)天，显•基础研究・南方医科大学南方医院肿瘤科，广东广州510515DepartmentofOncolog),NanfangHospital.SouthernMedicalUniversity,(；uangzhou,Guangdong,510515,P.R.China通讯作者：罗荣城Correspondenceto：LLORong-ChengTel：86-20-61641651E-mail:lrc@nnioc著短于生理盐水外涂组［(23.70±2.41)］天和模型对照组［(25.70±2.26)天］(PvO.Ol)。结论：透明质酸酶单独局部注射或联合儿丁精外涂均可减少多西紫杉醇外渗性损伤的发生，缩短损伤愈合时间。单用儿丁糖外涂不能防止损伤的发生，却可促进损伤愈合。关键词：几丁糖/治疗应用；透明质酸陆/治疗应用；多西紫杉醉；药物外渗；皮肤损伤；大鼠：R730.59文献标识码:A：1000-467X(2007)04-0346-05多西紫杉醇(Docetaxel)是一种新型抗肿瘤药，主要用于卵巢癌、乳腺癌、非小细胞肺癌及其他恶性...