•论著.自体干细胞注射治疗女性压力性尿失禁的实验研究%方祖军李映川徐可郑捷丁强【摘要】目的探讨自体肌肉干细胞注射治疗女性压力性尿失禁的i侧卵篥切除和反复阴逍扩张的方法，构建雌性SD大鼠压力性尿失禁动物模常对照组15只，尿失禁模型30只，其中模型对朋组15只，细胞注射治疗组建立模型后第2个月末接受自体肌肉干细胞尿道周圈注射治疗'注射治疗组治疗1个月后，测定3组大鼠腹压漏尿点压.同时取近段尿道组织，HE染色观察形态学变化。結果尿失蔡模型组腹压漏尿点压@=12)(26・4±2,7)cmH<0、正常对眼组14)(78.4士3.1)CTDH?。、细胞注射治疗组(n=ll)(58.8±2.4)cmH2O,3组间两两比较差异均有统计学意义。细胞注射后局部尿道壁肌层增厚、肌纤维增强。结论自体肌肉干细胞注射治疗能明显提高压力性尿失禁动物模型的腹压漏尿点压，并使注射位点尿道壁肌层得到增强。注射细胞数就和注射后对膀胱功能的远期影响需进~步研究。【关键词】女性,压力性尿失禁｝于细胞£xperimentalstidyofeffectsofautologousstemcellsinjectionforfemalestressurinaryincontinenceFANGZu-junfLIYing-chuan,XUKe9ZHENGJte,DINGQiang.DepartmentofUrology<HuashanHospital•FudanUniversitytShanghai200040.ChinaCorrespondingauthor:FANGZu-jun•Email*fangzujun321^163.com(AbstractJObjectiveToevaluatetheeffectsofmusclestemcellsinjectionforfemalestressur卜naryincontinence.MethodsFortyfivefemaleSpague-Dawleyratswereutilizedtodothisstudy.A-mongthem^30ratswereusedtomimicfemalestressurinaryincontinence.Group1wasnormalcon-trol(n=15),group2wastreatedwithbilateralovarectomyandrepeatedvaginaldilationasfemalestressurinaryincontinencemodelcontrolCn^15)>group3wastreatedwiththesamemanagementasgroup2initiallyand2monthslaterautologousmusclelstemcellswereinjectedaroundproximalurethral.Threemonthslater»alltheanimalsreceivedurodynamicmeasurementstoevaluatechangesofabdominalleakpointpressure.Proximalurethraltissueofeachratwasalsoharvestedandhematoxy-lin/eosinstainedResultsTheabdominalleakpointpressure(ALPP)ofgroup2(拜=12,26.4士2・7cmH2O)andgroup35=11.5&8土2.4cmH?O)wassignificantlylowerthanthatofgroupl(n—14,7&4土3ApmH?O).ALPPofgroup3wassignificantlyhigherthanthatofgroup2.However•theALPPofgroup3waslowerthanthatofgroupLUrethralmuscleofinjectedciteswasobviouslyenhancedtoo.ConclusionsOiltherapymediatedbymusclestemcellscouldenhancerheALPPofstressurinaryincontinenceanimalsignificantly.Itmaybeanewkindoftreatmentforfemalestressu-rinaryincontinenceinthefuture.Howeverrmorestudyshouldbedoneonthenumbersofinjectedcells>long-terminfluenceonthebladderfunctionandtoincreasenewinjectedcitestoimproveitseffectsandcontradiction.【Keywords]FemalejStressurinaryincontinencejStemcells基金项目，上梅市科委基金资助项目＜044119605)作者单位：200040上海，复旦大学附厲华山医院泌尿外科31佰作者：方祖军丫EmailJangxujun321@163.com3.6)g,治疗组无统计后正常我们利用组织工程技术，将体外扩增的自体骨骼肌卫星细胞注射到压力性尿失禁动物模型近段尿道壁肌层，探讨治疗女性压力性尿失禁的可能性，现报告如下。材料与方法一、动物分组和模型建立清洁级3周龄雌性SD大鼠45只，购于复艮大学实验动物部。随机分为正常对照组伍=15)、压力性尿失禁动物模型对照组G=15)和骨骼肌卫星细胞注射治疗组5=15)3组。采用双侧卵巢切除+反复阴道扩张构建动物模型。1%戊巴比妥腹腔麻醉,经腹部小切口切除双侧卵巢，缝合切口后，即用8FFoley气囊导尿管作初次阴道扩张，气囊注水3ml,扩张2h,导尿管末端悬吊150g牵引。每间隔1周阴道扩张1次，共3次。二、自体骨骼肌卫星细胞分离、体外扩增和尿道周围注射无菌条件下切取大鼠后肢骨骼肌组织1・0〜1・5g,采用改良两步酶消化法分离得到自体骨骼肌卫星细胞,DMEM培养芨体外培养•并加入IGF-K20ng/ml)扩...