—论著—miRNA5286LmiRNAS483j3p与多囊卵巢综合征患者内分泌代谢及卵母细胞质量的相关性*杨潇曼'，王克华i,王晓丹L马芋芋'，石家秀'，张明月'(1.山东中医药大学，济南250011;2.山东中医药大学附属医院中西医结合生殖与遗传中心，济南250011;3.莱阳市人民医院，莱阳265200)常摘要荥B的：探讨miRNA龄61、miRNAM83!3p在多囊卵巢综合征(PCOS)患者血清和颗粒细胞中的差异表达，及其对体外受精(IVF)过程中卵母细胞质量及胚胎发育潜能的影响。方法：收集2016年4月至2018年1月于山东中医药大学附属医院生殖中心行显微受精郅胚胎移植(ICSI)的PCOS患者58例(PC0S组)和单纯因男方因素不孕的患者23例(对照组).RTIPCR法检测PCOS组与对照组血清及颗粒细胞中miR!286hmiRB483I3p的表达水平’结果:rniR|286LmiRM83?3p在血清和颗粒细胞中表达水平具有一致性，PCOS组均低于对照组，差异有统计学意义(PC0.05)。PCOS组血清中miRJ2861表达水平与空腹胰岛素、总胆固醇呈负相关,PCOS组血清中miR5483黔p表达水平与睾酮、空腹胰岛素、低密度脂蛋白呈负相关(P<0.05)。PCOS组颗粒细胞中miR乾861表达水平与获卵率、MII卵率、2PN卵裂率、优质胚胎率呈正相关(PC0.05),PCOS组颗粒细胞中miRS48353p表达水平与获卵率、Mil卵率、正常受精率、2PN卵裂率、优质胚胎率呈正相关(P<0,05)。结论:miR纹861和miRM83l3p在PCOS患者血清、颗粒细胞中异常低表达，异常表达的miR!286hmiR5483!3p可能使卵母细胞及胚胎质量下降，间接影响IVF成功率，犊关键词铳多囊卵巢综合征;rniRNA52861;n)iRNAM83l3p;血清;颗粒细胞;胚胎质量中图分类号:R711.75文献标志码:A文章编号:1004-7379(2021)09-0666-05DOI:10.13283/j.cnki.xdfckjz.2021.09.004CorrelationbetweenmiRNA|2861,miRNAj483?3pandendocrinemetabolismandoocytequalityinpatientswithpolycysticovarysyndrome.YangXiaoman1,WangKehua?,WangXiaodan1,etal.1.ShandongUniversityofTraditionalChineseMedicine,Jinan250011;2.Inte?grativeMedicineCenterforReproductiveandHeredity,theAffiliatedHospitalofShandongUniver?sityofTraditionalChineseMedicine,Jinan250011:3.LaiyangPeople'sHospital,Laiyang265200犊Abstract抵Objective:ToinvestigatethedifferentialexpressionofmiRNAj2861andmiRNAj483?3pinserumandgranulosacellsinPCOSpatients,anditseffectonoocytequalityandembryodevelopmentpotentialduringinvitrofertilization(IVF).Methods:58PCOSpaltientsunderwentICSIfromApril2016toJanuary2018wereenrolledasihestudygroup(PCOSgroup),while23infertilepatientswithmalefactorsasacontrolgroup.TheexpressionlevelsofmiRj2861andmiR?483j3pinserumandgranulosacellsofallgroupsweredetectedbyRTiPCR.Results:TheexpressionlevelsofmiRj2861,miRM83j3pinserumandgranulosacellswerelowerinthePCOSgroupthanthoseinthecontrolgroup.Thedifferencewassignificant(P<0.05).TheexpressionlevelofmiR52861inserumofPCOSgroupwascorrelatedwithfastinginsuJnandtotalcholesterolnegatively.TheexpressionlevelofmiR!483S3pinserumofPCOSgroupwascorrelatedwithtestosterone,fastinginsulinandlowdensitylipoproteinnegaiively(P<*国家自然科学基金(No:81573778)*通信作者Email:sdwangkh@126.com6660.05).ThelevelofmiRS2861ingranulosacellsofPCOSgroupwaspositivelycorrelatedwitheggcapturerate,Mileggrate,2PNcleavagerateandhighqualityembryorate(P<0.05).ThelevelofmiR!483l3pingranulosacellsofPCOSgroupwaspositivelycorrelatedwitheggcapturerate,Mileggrate,normalfertilizationrare,2PNcleavagerateandhighqualityembryorate(P<0.05).Conclusion:TheexpressionsofmiRf2861andmiRj483j3paresignificantlowerinserl腊H廊阳叫'(龊§禺侦曲醐沾钠而偶占如财腹滞『忤卬哧戒8装宙时沏平弓海醐贫施creaseHKeywords兼Polycysticovaryyndrome；miRNA/861；miRNA|183?3以SerumGranulolsacells;Embryoquality多囊卵巢综合征（polycysticovariansyndrome,P5COS）是一种以雄激素...