中国组织工程研究与临床康复第14卷第50期2010–12–10出版JournalofClinicalRehabilitativeTissueEngineeringResearchDecember10,2010Vol.14,No.50ISSN1673-8225CN21-1539/RCODEN:ZLKHAH93781DepartmentofOrthopaedics,Bei激ngTongrenHospital,CapitalMedicalUniversity,Bei激ng100730,China;2DepartmentofBiochemistryandMolecularBiology,BasicResearchInstitute,ChineseAcademyofMedicalSciences,Bei激ng100005,China;3InstituteofSpinalSurgery,ChangzhengHospital,theSecondMilitaryMedicalUniversityofChinesePLA,Shanghai200003,ChinaQiangHua,Doctor,Associatechiefphysician,Departmentof☆Orthopaedics,Bei激ngTongrenHospital,CapitalMedicalUniversity,Bei激ng100730,ChinaQh新疆_cn@sinaReceived:2010-07-24Accepted:2010-10-20---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---应用cDNA微阵列分析脊髓损伤后基因表达的差异☆强华1,周严2,刘瑾2,陈雄生3,黄晓伟2,张斌2,阴彬2,袁建刚2,贾连顺3AnalysisofgeneexpressiondifferencefollowingspinalcordinjuryinratsusingcomplementaryDNAmicroarrayQiangHua1,ZhouYan2,Liu激n2,ChenXiong-shen3,HuangXiao-wei2,ZhangBin2,YinBin2,Yuan激an-gang2,激aLian-shun3AbstractBACKGROUND:Genemicroarraycanbeusedtoparalleldetectexpressionpatternsoflargeamountsofgenes,whichovercomethelimitationoftraditionalonlysingleormultiplegenesdetection.OBJECTIVE:Todynamicobservethechangesofgeneexpressioninratacutespinalcordinjury(SCImodelsusingcomplementaryDNAmicroarrayconsisting1176genes.METHODS:SeventyfemaleSDratswererandomlydividedintothenormalcontrol,surgerycontrol,4-,24-hourand3-,7-,10-dayinjurygroups.RatsintheinjurygroupssubjectedtoT7andT8excision,andmadeSCImodelsbyhighfalling.Shamanimalsreceivedonlyalaminectomy.T6-10spinalcordwasharvestedateachtimepoint,andautoradiographicgeneexpressionprofilewasanalyzedbyAtlasImageTM2.01software(Clontechparedwiththenormalgroup,greaterthan3-foldchangesconsideredasdifferentialexpression.RESULTSANDCONCLUSION:Weidentified81genesthatshowedagreaterthan3-foldchangeinSCItissues,including46genesupregulatedand35genesdownregulated.Inaddition,changesofneurokininB,neuropeptideYandpostlobin-v2receptorgenewerefirstlyfoundduringSCI.Theresultsdemonstratedthat,geneexpressionpr---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---ofileduringacuteSCIcanbeobservedbyusinggenemicroarraycombinedwithexperimentalanimalmodels,whichhassignificanceforfurtherpathogenesisstudyatgenelevels.QiangH,ZhouY,LiuJ,ChenXS,HuangXW,ZhangB,YinB,YuanJG,激aLS.AnalysisofgeneexpressiondifferencefollowingspinalcordinjuryinratsusingcomplementaryDNAmicroarray.ZhongguoZuzhiGongchengYan激uyuLinchuangKangfu.2010;14(50:9378-9381.[wwcrteren.zglckf]摘要背景:基因芯片可以大规模地平行检测分析上千个基因的表达模式,克服了传统的一次实验仅能对单个或数个基因表达水平进行分析的局限。目的:应用含有1176条人类全长基因的cDNA表达谱芯片,对大鼠急性脊髓损伤模型中基因表达水平的变化进行动态观察。方法:雌性SD大鼠70只随机分成正常对照组、手术对照组、损伤4h,24h,3d,7d,10d组7组。损伤组切除T7、T8的椎板,并用钢棒从高处自由落下致脊髓损伤。手术对照组仅进行T7、T8椎板切除。正常组和各损伤组于伤后各时间段,手术对照组于术后3d取T6~T10段脊髓,提取总RNA,运用AtlasImageTM2.01软件(Clontech对放射自显影基因表达谱进行分析,各个处理组与正常组相比,灰度值差异超过3倍的基因定为有表达差异。结果与结论:结果显示共有显著表达差异基因81条,其中表达上调的基因有46条,表达下调的基因有35条,并在国内外首次观察到神经激肽B、神经肽Y、垂体后叶加压素V2受体等数个基因...