参附对失血性休克大鼠肝脏TF，TM，t-PA，PAI-1mRNA表达的影响【摘要】目的：探讨失血性休克大鼠肝脏组织组织因子(TF)、血栓调节蛋白(TM)、组织型纤溶酶原激活剂(t-PA)和纤溶酶原激活剂抑制物-1(PAI-1)的表达情况以及参附注射液的干预作用。方法：成年健康SD大鼠分成4组：对照组、休克组、林格液组、参附液组。对照组常规饲养，其余3组大鼠复制失血性休克模型。休克组不予治疗，林格液组用3倍失血量的林格液治疗，参附组用含参附注射液(10mL/kg)和林格液组成的3倍失血量的液体治疗。治疗后2h取肝脏组织，－70℃保存，用逆转录多聚酶链反应（RT-PCR）法检测肝脏组织中t-PA，PAI-1和TF，TMmRNA的表达。结果：①休克组肝脏组织TF，TM，t-PA，PAI-1mRNA表达与对照组相比明显升高（P《0.05）。②林格液组肝脏组织TF，TM，t-PA，PAI-1mRNA表达与对照组及休克组比较明显升高(P《0.05)。③参附液组肝脏组织TF，TM，t-PA，PAI-1mRNA与对照组相比较，差异无显著性，与休克组及林格液组比较表达有降低，差异有显著性(P《0.05)。结论：休克状态下肝脏组织及内皮细胞损伤持续存在，参附注射液可使凝血活性下降，同时又可防止纤溶过度亢进。【关键词】失血性休克组织因子血栓调节蛋白组织型纤溶酶原激活剂纤溶酶原激活剂抑制物-1参附注射液Abstract:Objective:Toexploretheexpressionoftissuefactor(TF)，thrombomodulin(TM)，tissueplasminogenactivator(t-PA)，plasminogenactivatorinhibitor-1(PAI-1)inratliverafterhemorrhagicshock，andtheeffectofShenfuinjectiononthem.Methods:Sprague-Dawleyratsweredividedintofourgroups:controlgroup，hemorrhagicshock(HSgroup)，resuscitationwithRinger’ssolution(RSgroup)，andresuscitationwithShenfuinjection(SFgroup).Theratsincontrolgroupweretreatedwithregularfeeding；therestgroupsweresubjectedtohemorrhagicshock.Then，HSgroupwasleftwithouttreatment，RSgroupwastreatedwithRinger’ssolutionwiththreeofvolumeoflostblood，SFgroupwastreatedwithmixtureofShenfuinjection(10mL/kg)andRinger’ssolutionwiththreeofvolumeoflostblood.Theliverswerecollectedafter2hoursofthetreatmentandstoredat-70℃.ThemRNAlevelsoftissueplasminogenactivatorantigen(t-PA:Ag)，plasminogenactivatorinhibitor-1antigen(PAI-1:Ag)，thrombomodulin(TM)andtissuefactor(TF)weremeasuredbyreversetranscriptionpolymerasechainreaction(RT-PCR).Results:Comparedtothecontrolgroup，mRNAlevelsofTF，TM，t-PA，PAI-1mRNAofHSgroupinliverweresignificantlyincreased(P《0.05)；ThemRNAlevelsofTF，TM，t-PA，PAI-1inRS---本文于网络，仅供参考，勿照抄，如有侵权请联系删除---groupincreasedsignificantlycomparedtothoseofcontrolgroup(P《0.05)andHSgroup(P《0.05).ThemRNAlevelsofTF，TM，t-PA，PAI-1inSFgroupweresignificantlylowerthanthoseofRSgroupandtheHSgroup(P《0.05)，buttherewasnotstatisticaldifferencecomparedtothecontrolgroup.Conclusion:Whenhemorrhagicshockhappens，theinjuryofliverandendothelialarepersistentlyexist.Shenfuinjectioncanpreventtheformationofthrombus，improvemicrocirculationandlessbleeding.Keywords:hemorrhagicshock；tissuefactor；thrombomodulin；tissue-plasminogenactiv-ator；plasminogenactivatorinhibitor-1；Shenfuinjection多年来，国内外关于休克的研究主要集中在炎症因子和抗炎治疗上。由于炎症与机体的凝血和抗凝血的动态平衡关系密切，抗凝也逐渐成为休克治疗新的靶点[1]。本实验观察了失血性休克时肝脏组织的组织因子(tissuefactor，TF)、血栓调节蛋白(thrombomodulin，TM)、组织型纤溶酶原激活剂(tissuetypeplasminogenactivator，t-PA)和纤溶酶原激活剂抑制物-1(plasminogenactivatorinhibitor-1，PAI-1)等参与凝血、纤溶过程的物质的表达情况，以及参附注射液的干预作用，为临床上对失血性休克的治疗提供实验依据。1材料和方法1.1材料1.1.1实验...