甘露消毒丹及其拆方对感染肠道病毒71型细胞miR―146a和Toll样受摘要：目的探?甘露消毒丹及其拆方对肠道病毒71型（EV71）感染后免疫及炎症反应的调节作用。方法运用细胞培养技术，设甘露消毒丹组（甘全方组）、清残方组、利残方组、病毒唑组、正常组、模型组，进行病毒毒力和药物细胞毒性测定后，以药物作用于EV71感染后的RD细胞，24h后RT-PCR检测各组细胞miR-146a、Toll样受体4（TLR4）mRNA表达。结果与正常组比较，模型组miR-146amRNA表达降低，TLR4mRNA表达升高；与模型组比较，甘全方组、清残方组、利残方组miR-146amRNA表达升高、TLR4mRNA表达降低，病毒唑组与模型组比较差异不明显；与甘全方组比较，清残方组miR-146a、TLR4mRNA表达降低，利残方组miR-146amRNA表达升高、TLR4mRNA表达降低；与清残方组比较，利残方组miR-146amRNA表达升高，TLR4mRNA表达无明显差异。结论甘露消毒丹可能通过调节miR-146a、TLR4mRNA表达从而影响EV71感染所致免疫反应，其中清残方与利残方可能存在互相拮抗效应。关键词：甘露消毒丹；拆方；肠道病毒71型；miRNA-146a；Toll样受体4DOI：10.3969/j.issn.1005-5304.2017.03.015：R285.5文献标识码：A：1005-5304（2017）03-0061-05EffectsofGanluXiaoduDanandItsIncompletePrescriptiononExpressionsofMiR-146aandTLR4mRNAinCellsInfectedbyEV71AIBi-chen1，HEYi-rong1，CAORong1，HEDong2，ZHAOGuo-rong1，HEYou-shun1（1.TCMSchoolofHunanUniversityofChineseMedicine，Changsha410208，China；2.GraduateSchoolofHunanUniversityofChineseMedicine，Changsha410208，China）Abstract：ObjectiveToobservetheeffectsofGanluXiaoduDananditsincompleteprescriptiononexpressionsofMiR-146aandTLR4mRNAinRDcellsinfectedbyEV71.MethodsWithtechniqueofcellculturing，GanluXiaoduDantherapygroup，incompleteQingprescriptiontherapygroup，incompleteLiprescriptiontherapygroup，normalcellscontrolgroup，modelcontrolgroupandribavirincontrolgroupwereset，andtestsforvirustoxicityandmedicinetoxicityincellsweretaken，thenexpressionsofmiRNA-146aandTLR4mRNAintheseRDcells24hoursafterinterventionwithmedicineweredetected.ResultsComparedwithnormalcellscontrolgroup，miR-146ainmRNAmodelcontrolgroupdecreasedandTLR4mRNAincreasedparedwithmodelcontrolgroup，miR-146amRNAinGanluXiaoduDantherapygroup，incompleteQingprescriptiontherapygroupandincompleteLiprescriptiontherapygroupallincreasedwhileTLR4mRNAdecreased，anddifferencesbetweenribavirincontrolgroupandmodelcontrolgroupwerenotsignificantparedwithGanluXiaoduDantherapygroup，bothexpressionsofmiR-146aandTLR4mRNAinincompleteQingprescriptiontherapygroupwerelower；miR-146aincreasedandTLR4mRNAdecreasedinincompleteLiprescriptiontherapygroupparedwithincompleteQingprescriptiontherapygroup，miR-146amRNAinincompleteLiprescriptiontherapygroupincreased，butexpressionofTLR4mRNAbetweenthemwasnotsignificant.ConclusionGanluXiaoduDancanregulatetheimmunereactionscausedbyinfectionofEV71byincreasingexpressionofmiR-146amRNAandreducingexpressionofTLR4mRNA.TheremaybeantagonismeffectbetweenincompleteQingprescriptionandincompleteLiprescription.Keywords：GanluXiaoduDan；incompleteprescription；EV71；miRNA-146a；TLR4基金项目：国家自然科学基金（81173188）；湖南中医药大学科技创新团队项目（2016年）通讯：贺又舜，E-mail：youshunh@sina手足口病是由多种肠道病毒引起的一种婴幼儿和儿童常见传染病，近年手足口病的发病率及病死率有增高趋势[1]。肠道病毒71型（EV71）及柯萨奇病毒A组16型（CoxAl6）为手足口病最主要的致病病毒，其中重症手足口病病情进展快，死亡率较高，多由EV71感染引起，致死原因主要为脑干脑炎及神经源性肺水肿。目前缺乏特异高效的抗手足口病病毒药物，对手足口病尚无特异性治疗方法，主要以对症...